experiments animals science

Hands-On Science Experiments for Kids About Animal Adaptations

How do animals adapt and survive in their environment? How can a polar bear survive living in cold weather? Children tend to have a natural curiosity about the world and animals in it. There are lots of fun science experiments for kids to learn about animal adaptations and all the special features animals have to help them survive.

In the winter, polar bears are always a topic that comes up in my classroom. Children feel how cold the snow and winter weather is for them and they naturally wonder about how animals who thrive in the cold are able to do it.

Instead of simply telling children how animals adapt to the cold and different conditions, you can show them with this science experiment about animal adaptions for kids.

As a basic explanation, polar bears bodies are different from ours. Their bodies have adapted to the cold and include special features that make it so that they can live in places with snow and cold weather.

Under a polar bears skin, they have a thick layer of blubber, or fat, that helps keeps them warm. Kind of link their own heavy winter jacket. This experiment will show children exactly what that layer of blubber really does as children experience it hands-on.

Science Experiments for Kids -The Materials

This experiment only requires a few materials. You will be creating a mitt to simulate the layer of blubber that a polar bear has on its body.

• Shortening / Lard (Room Temperature)
• Plastic Baggies
• Strong, Thick, Waterproof Tape
• Large Bucket with Water

Once you have all of your materials, you are ready to create your polar bear mitt.

Creating Your Mitten

Start by putting some of the shortening into one of the plastic bags. Fill the bag about 3/4 full.

Next, insert another plastic bag into the bag with the shortening. Make sure that none of the shortening gets into this bag. Keep your hand in the bag and squish the shortening around the bag so that it is surrounded on all sides.

You should be able to put your hand into the inside bag without touching any shortening.

Next, fold down the top of the inside bag so that it covers over the top of the bag filled with shortening. Once folded, use the tape to seal around the top. Make sure that the tape covers and seals the tops of the bags so that no shortening can squeeze out.

Apply a few layers if needed.

Your polar bear mitten is complete!

Science Experiments for Kids – The Steps

Once you have created your mitten, you are ready to try out the experiment! Since I did this experiment at school with a lot of children, I created several mitts so that more then one child could do the experiment at once.

I started by showing the children a bit of the shortening. We talked about how polar bears have a layer of something similar under their fur. Children predicted how they thought that would help them to stay warm.

Once everyone had predicted, I then showed the mittens that I had made.

If it is an option, having children create the mitts can also be a lot of fun for them and more hands-on learning. My kids helped me create them at home and enjoyed the process.

Next, I brought out the large bucket of ice water. I made sure to have a few ice cubes still floating on top to show how cold the water was.

I then had one child at a time come up and put on the mitt. With the mitt on they then put their hand with the mitt, and a bare hand into the water.

(Make sure that children only leave their bare hand in the water for a few seconds!)

The effect of the mitt with the shortening is obvious immediately.

Everyone had a chance to test out the mitt. Very quickly children noticed that the hand with the mitt was protected from the cold. Within seconds, everyone had to take out their bare hand because it was so cold.

However, the hand inside the mitt was fine to stay in the ice cold water. The shortening, or blubber, protected their hand from the cold and kept it warm.

I love the idea of kids being able to feel the protection that a bear has when it builds up its layers before hibernating.  The polar bear has adapted to their environment and can easily survive in the cold.

At home, my own children had a blast seeing how long they could keep their hand with the glove, in the water and then understanding, how polar bears, who are covered with a similar insulation, are able to survive the cold.

Science experiments for kids are a great way for children to learn something hands-on in a meaningful way that they will remember. This experiment is a really easy way to help show children this animal adaptation.

Extension Ideas and Tips

Although the edges are sealed and secure, if children are careful and don’t squish or pick at the bags, you can freeze them for later and use them again.

Science experiments for kids are a great way to teach children about animal adaptations. After completing this experiment, you can discuss with children what other adaptations they think polar bears, or other animals, have to help them survive.

Children may notice that a lot of animals who live in cold, snowy areas are white. Point out that this is an adaptation because the white helps them to camouflage and survive.

Animals and their adaptations are fascinating! Finding creative ways to teach about animal adaptations for kids is simple and rewarding!

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Animal Studies and School Project Ideas

From Science Fair Project Ideas on Mammals to Experiments About Insects

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• Cell Biology
• Weather & Climate
• B.A., Biology, Emory University
• A.S., Nursing, Chattahoochee Technical College

Animal research is important for understanding various biological processes in animals , humans included. Scientists study animals in order to learn ways for improving their agricultural health, our methods of wildlife preservation, and even the potential for human companionship. These studies also take advantage of certain animal and human similarities to discover new methods for improving human health.

Learning From Animals

Researching animals to improve human health is possible because animal behavior experiments study disease development and transmission as well as animal viruses . Both of these fields of study help researchers to understand how disease interacts between and within animals.

We can also learn about humans by observing normal and abnormal behavior in non-human animals, or behavioral studies. The following animal project ideas help to introduce animal behavioral study in many different species. Be sure to get permission from your instructor before beginning any animal science projects or behavioral experiments, as some science fairs prohibit these. Select a single species of animal to study from each subset, if not specified, for best results.

Amphibian and Fish Project Ideas

• Does temperature affect tadpole growth?
• Do water pH levels affect tadpole growth?
• Does water temperature affect amphibian respiration?
• Does magnetism affect limb regeneration in newts?
• Does water temperature affect fish color?
• Does the size of a population of fish affect individual growth?
• Does music affect fish activity?
• Does the amount of light affect fish activity?

Bird Project Ideas

• What species of plants attract hummingbirds?
• How does temperature affect bird migration patterns?
• What factors increase egg production?
• Do different bird species prefer different colors of birdseed?
• Do birds prefer to eat in a group or alone?
• Do birds prefer one type of habitat over another?
• How does deforestation affect bird nesting?
• How do birds interact with manmade structures?
• Can birds be taught to sing a certain tune?

Insect Project Ideas

• How does temperature affect the growth of butterflies?
• How does light affect ants?
• Do different colors attract or repel insects?
• How does air pollution affect insects?
• How do insects adapt to pesticides?
• Do magnetic fields affect insects?
• Does soil acidity affect insects?
• Do insects prefer the food of a certain color?
• Do insects behave differently in populations of different sizes?
• What factors cause crickets to chirp more often?
• What substances do mosquitoes find attractive or repellent?

Mammal Project Ideas

• Does light variation affect mammal sleep habits?
• Do cats or dogs have better night vision?
• Does music affect an animal's mood?
• Do bird sounds affect cat behavior?
• Which mammal sense has the greatest effect on short-term memory?
• Does dog saliva have antimicrobial properties?
• Does colored water affect mammal drinking habits?
• What factors influence how many hours a cat sleeps in a day?

Science Fun

How Do Animals Hide Animal Science Experiment

Animals hide and use camouflage as an important tactic to survive and avoid predators. In this fun and easy animal science experiment, we are going to demonstrate the effectiveness of camouflage and hopefully provide some understanding into how it helps animals hide.  

Two sheets each of four different colored pieces of construction paper.

Instructions:

• Take one sheet of each color of construction paper. Cut each sheet into 2 inch by 2 inch square pieces.
• Ask your friend to come over and have them close their eyes.
• Place the colored squares onto one of the large sheets of construction paper.
• Have your friend open their eyes.
• Specify a color that is no the color of the big piece of construction paper and give them five seconds to pick out as many squares as they can in the allotted time.
• Now try again, this time give them five seconds but choose the color that matches the large piece of construction paper. 

EXPLORE AWESOME SCIENCE EXPERIMENT VIDEOS!

How it Works:

Camouflage is a tactic animals use to blend in with their surroundings. This animal science experiment is designed to demonstrate the difference is identifying items that do not blend in with their surroundings, as opposed to those that do blend in with their surroundings. 

Make This A Science Project:

Use some white animal bedding for hamster cages and hide several cotton balls inside. Add some colored pompoms and other similar sized and colored items to the bedding. Give a friend an allotted mount of time to recover items from the bedding. Keep a record of the amount and type of items found. Now try again but this time tell your friend to only search for cotton balls. Does this effect the amount of cotton balls recovered? 

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Hands On As We Grow®

Hands on kids activities for hands on moms. Focusing on kids activities perfect for toddlers and preschoolers.

50 Amazingly Simple Science Experiments for Kids at Home

Science Kindergartners Preschoolers Experiment Resources 30 Comments

Kids love experimenting , and these 50 simple science experiments for kids at home from Brigitte are perfect for all ages! Plus, you probably already have the basic supplies at home.

My daughters and I have had a lot of fun doing science experiments. Each year when we create our spring and summer list , we make sure to include “science days” which are days filled with science experiments.

Sometimes our science experiments don’t work according to plan, but I have been told that all scientists have failures with experiments from time to time.

It’s okay if they aren’t all successes.

Get the FREE Science Experiments Download

50 Simple Science Experiments with Supplies You Already Have

I love these 50 simple science experiments for you to try with your little scientists. They all use basic household supplies that you probably already have at home!

Most of these are experiments my daughters and I have done together. I hope you enjoy them as much as we have!

Get little ones involved with these easy toddler-friendly science experiment ideas!

Simple Science Experiments with Water

Not only can water  be a blast to play in, but water plus a few basic supplies equals a lot of science fun!

• Make an orange sink and float with an orange buoyancy experiment from Playdough to Plato.
• Compare the amount of salt in different types of water with this salty egg experiment  as seen on Uplifting Mayhem.
• Do a little more sinking or floating with a fun sink or float experiment  even toddlers can do from Hands On As We Grow.
• Use the free printable to record what sinks or floats in an outdoor experiment from Buggy and Buddy.
• Create some beautiful pieces of paper with this rainbow paper experiment from Science Kiddo.
• Talk about solutions as you try the “what dissolves in water” experiment  as seen on Hands On As We Grow.
• Learn about water absorption with this simple experiment from Little Bins for Little Hands.
• Mix some fun colors with this oil and water experiment  from Fun Learning for Kids.
• Make your own lava lamp , just like on  Hands On As We Grow.
• Can you keep all the water in the bag? Try it with a  leak-proof bag experiment  as seen on Hands On As We Grow.
• Learn about surface tension with this  magic finger pepper experiment  found on Hands On As We Grow.
• Make your own  water cycle in a bottle  as seen on A Dab of Glue Will Do.

Simple Science Experiments with Baking Soda and Vinegar

Baking soda + vinegar = a great chemical reaction! This fizzy reaction can fuel a variety of simple science experiments at home.

First of all, we have tested and found out the absolute best combination of baking soda and vinegar to get the best reaction possible. It makes a difference if you add vinegar to baking soda or vice versa! And how much you use!

• Inflate a balloon without blowing into it with a baking soda and vinegar balloon experiment  as seen on Little Bins for Little Hands.
• Practice colors as you do a baking soda and vinegar with color experiment as seen on Hands On As We Grow.
• Have fun outside with an outdoor volcano eruption  as seen on Preschool Inspirations.
• Have more volcano fun by making apple volcanoes as seen on The Resourceful Mama.
• Learn about acids and bases and the chemical reaction that occurs when you make apple seeds dance with a   jumping apple seeds experiment  as seen on JDaniel4s Mom.
• Watch some rice dance with a   dancing rice experiment as seen on Green Kid Crafts.
• Continue your dance party by making raisins dance with a dancing raisin experiment  as seen on 123 Homeschool 4 Me. What other items can you get to dance?
• Learn more about acids and bases by dissolving a sea shell as seen on Teach Beside Me.
• Make an egg shell disappear with this disappearing egg activity  as seen on Premeditated Leftovers.
• See how far you can launch a soda bottle with this baking soda powered boat as seen on Science Sparks.
• Make your own rocks (or eggs) with this fizzy treasure rocks experiment as seen on Living Life and Learning.
• Have some fun this summer with this frozen vinegar experiment as seen on Inspiration Laboratories.

Plant Themed Simple Science Experiments

Enjoy learning about seeds, plant parts, and how plants grow with these simple science experiments.

• Learn about how plants soak up water through their stems with a flower experiment for kids  from Growing A Jeweled Rose.
• Watch seeds sprout as you grow seeds in a jar  as seen on Teaching Mama.
• Learn about the parts of the seed with a seed coat experiment as seen on Gift of Curiosity.
• Build a house out of sponges and then watch it sprout with this sprout house as seen on The Stem Laboratory.
• Learn what liquids allow seeds to grow the best with this seed experiment  as seen on Gift of Curiosity.
• Explore how plants grow towards the light with this shoe-box maze experiment from Plants for Kids.

Animal Themed Simple Science Experiments

Learning about animals can be even more fun with some simple hands-on simple science experiments.

• Find out more about giraffes and create some giraffe spots  as seen on Preschool Powol Packets.
• Learn about how animals in the Arctic keep warm by making an arctic glove  as seen on Steve Spangler Science.
• Discover how penguins stay dry with a penguin feather experiment as seen on Raising Little Superheroes.
• Learn about different bird beaks with a bird beak experiment as seen on Blessed Beyond a Doubt.
• Explore how fish (and hermit crabs) breathe with this gill experiment  as seen on Preschool Powol Packets.
• Learn about sharks with a   shark buoyancy experiment as seen on Little Bins for Little Hands.

Even More Simple Science Experiment for Kids at Home!

If you are still looking for more science fun, you may enjoy the following simple science experiments.

• Find out how sugary drinks hurt teeth with an  eggs-periment  as seen on Feels Like Home Blog.
• Discover geodes (the state rock of Iowa) with this eggshell geode crystal experiment  as seen on Science Bob.
• Learn about air pressure with an egg and bottle experiment  as seen on Science Sparks.
• Find out what causes an apple to brown with this apple science experiment  as seen on Teach Beside Me.
• Make an  edible bubble apple with an experiment as seen on Preschool Powol Packet.
• Learn more about surface tension with a penny and water experiment  as seen on Artful Parent.
• Mix colors like magic with this color changing milk experiment  from Hands On As We Grow.
• Blow up a balloon with this soda and balloon experiment from Learn Play Imagine.
• Practice letters by making beautiful crystal letters as seen on Books and Giggles.
• Make your own indoor hovercraft  as seen on Living Life and Learning.
• Learn about colors with this beautiful butterfly chromatography craft  as seen on Buggy and Buddy.
• Make soap souffle  as seen on Steve Spangler Science.
• After talking about liquids and solids (and finding them in your own home), create oobleck  as seen on Babble Dabble Do. Is it a liquid, or is it a solid?
• Learn about frost by making some indoor frost as seen on Little Bin for Little Hands.
• Make your own homemade butter in a jar as seen on Happy Hooligans.

What scientific experiment will you try first?

About Brigitte Brulz

Brigitte Brulz is a homeschooling mom of two daughters, wife of her high school sweetheart, and author of Jobs of a Preschooler and Pickles, Pickles, I Like Pickles. She offers free coloring pages and activity ideas on her website at BrigitteBrulz.com .

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Reader Interactions

30 comments.

college brawl says

March 13, 2024 at 1:05 am

Wow, these experiments look like so much fun! I can’t wait to try them out with my kids. We’re always looking for new and creative ways to learn about science at home, and these experiments look like they’ll be perfect for us. Thanks for sharing! 😊

threadsBay says

August 31, 2023 at 3:13 am

I love science experiments! This one is really simple and easy to do.

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Animal Science for kids – Worksheets, Experiments, & Activities

Animals for kids.

If you are teaching your child about  animal science for kids , you will want to take a peak at these  animal experiments and activities! Whether you are learning about  – we’ve got science worksheets and projects to help kids learn. Use these ideas to round-out your animal lessons with preschool, pre-k, kindergarten, first grade, 2nd grade, 3rd grade, 4th grade, 5th grade, 6th grade, 7th grade, 8th grade, 9th grade, 10th grade, 11th grade, and 12th grader.

Animal Science for kids

Our family loves using the above curriculum, but these animal activities and  animal for kids science ideas will work with whatever Botany or Biology Science curriculum you are using. Simply scroll below to find the science worsheets and earth experiments to round out your lesson. These biology for kid ideas are perfect for preschoolers, kindergartners, grade 1, grade 2, grade 3, grade 4, grade 5, grade 6, grade 7, grade 8, grade 9, grade 10, grade 11, and grade 12 students.

Animal Science

• Animals and their Babies Matching Game
• Animal Homes Clip Cards
• Farm Animals and their Homes Flip Book
• Nocturnal Animal Game for Kids
• Zoo Animal Reports
• Free Zoo Animals Worksheet – several leverls to choose from toddler, prek, elementary and middle school
• Jello Animal Cell Project
• Animal Habitats Activity with Animal Cookies
• Silly Animals and their Habitats Activity
• Animal Habitats Worksheets to complete with circle stickers
• Biomes Coloring Pages
• Animal Cookie Matching Activity

Animal Activities for Kids

• Jello Animal Cells Project
• Silly Puzzles Animal Habitat Activity
• Nocturnal Animals Printables
• Endangered Animals Worksheet s
• Forest Animals Themed Worksheets
• Arctic Animals Themed Worksheets
• Desert Animals Themed Worksheets
• Pond Worksheets for Kids
• Turkey Life Cycle Worksheets
• Life Cycle of Salmon Worksheets
• Life Cycle of an Ant
• Free Bat Life Cycle Worksheets
• Ladybug Life Cycle Worksheets
• Hatching Butterflies with Free Butterfly Printable worksheet and observation journal
• Life Cycles for Kids – a growing collection with over 20 different animals to explore from penguins to sunflowers, salmon, butterfly, and more!
• Animal Habitat Game

Animal Printables

We have lots of fun, hands on activities to teach kids about animals! Whether you are completing a zoology lesson , supplementing your homeschool science lesson, or just learning about your child’s favorite animals, we’ve got some fantastic resources filled with cute clipart and engaging activities:

• Free Animal Classifications for Kids Cootie Catchers
• Zoo Scavenger Hunt (includes simple picture clues for Pre k and more complex animal classification, biomes, endangered threat, etc for older elementary age students
• Animal Classification Worksheets to cut & paste
• Free Hibernation Printables
• Shark for Kids Printble Reader
• Endangered Animals Worksheet
• Ocean Animals for Kids – over 240 pages organized into 7 lessons to study aquatic animals using easy-to-understand text, hands-on science experiments, fun worksheets, and more with K-8th grade students.
• Animal Habitat Worksheets – Do a Dot Printable
• Jungle Writing Prompts Worksheets for kids

Arctic Animal Science Experiment for Preschoolers

Activities » Science » Arctic Animal Science Experiment for Preschoolers

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Do you have a preschooler at home who loves all things related to science experiments ? Then this Arctic animal-themed experiment is the perfect way to keep your little one entertained with an educational and fun activity!

In this simple experiment, your child will learn about six of the animals that inhabit the icy tundra of the Arctic while creating their own mini-habitat.

Through exploring how these animals survive in such extreme conditions, children can gain valuable insight into how certain species thrive in adverse environments and increase their appreciation for our beautiful natural world.

Like many four- and five-year-olds, my sons can’t get enough of animals and animal science.  Lately, their minds are all over winter and cold climate animals. Arctic animals are some of the coolest (ha!) out there and are super fun for learning with kids.

You name the animal. They are curious and want to learn more…

…and more…

So when my oldest son began asking questions about animals that live in the Arctic and why these animals don’t get cold, I immediately thought about Steve Spangler’s Blubber Gloves. So, I had to try this animal science experiment.

Easy & Fun Animal Science Experiment

Cold Climate Animals

The animals that live in the polar regions are fascinating to me. The birds and mammals that find a way to adapt are extraordinary. Below is a short list of a few well-known arctic animals.

• Arctic Hare
• Spotted Seal
• Arctic Wolf
• Beluga Whale
• Snowshoe Hare

Animals of the Polar Region Set of 3-Part-Cards

I love Steve Spangler, and I love all his work. He is a fantastic resource, but I wasn’t about to fill quart-sized plastic bags with shortening. I could see the shortening smeared on the bathroom mirror, on my sensory-seeking son’s legs, arms, and face.

Don’t get me wrong, I am all for messes but not on this particular day. I knew there had to be another way to get to an animal science experiment that would satisfy my sons’ tactile desires and my hope to keep the shortening off our furniture.

Then I recalled a science experiment book we’d recently taken from the library called Janice VanCleave’s Play and Find Out about Nature: Easy Experiments for Young Children. This book had a similar experiment (and many other fantastic and EASY science experiments for young children. I highly recommend it for animal science and all science learning.)

Animal Science Experiment

Materials for Animal of the Arctic Science Activity

• Shortening (the animal fat)
• Two plastic bags (the animal skin)
• Two ice cubes (the cold)

Approach to Animal Science Experiment

• Scoop out a tablespoon of shortening
• Put the shortening in the palm of your child’s hand
• Place hands – on with shortening and one without shortening – in the sandwich bags
• Now comes the ice cubes or crushed ice. Place on the outside of the bags on each hand.
• Have the child hug the ice cube with his hand.
• What do you observe?
• Why does the shortening or fat keep the cold out?

Science Behind the Activity

Shortening is a fat that insulates the cold. It is a thermoregulator, which keeps the warmth in and the cold out. Think about the fat substance. It is dense, right? It has low thermal conductivity relative to water.

So, even when in super cold water, the fat maintains its temperature. Fat doesn’t require a lot of blood supply, so blood stays closer to the skin’s surface, the surface closest to the coldness.

Extensions to this Activity

• Use different substances like butter, cheese, yogurt, fabric, foil, cotton, etc. Make a chart or graph of the observations.
• Use the hands-on sensory experience as a starting point to write and illustrate a story.
• Use ice in other ways to experiment with melting, chipping, and painting.
• Arctic animal sorting activity
• Play and feel the shortening. Spread it on your hands. Run your hands underwater. What do you observe?

I hope you enjoyed this post today!

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40 Animal Habitats Project Ideas & Science Activities

By: Author Jacquie Fisher

Posted on Published: January 20, 2021

Categories Kids Activities & Crafts , Science Experiments

The topic for this week’s Dr. Seuss science activities for kids is animal habitat project ideas and activities!

Diving in to learning about where animals live — like our recent layers of the ocean zones habitat project for kids   — is a wonderful way to inspire an interest in animals and their environment.

40 Animal Habitat Project Ideas & Activities

While kids love to observe animals, they usually don’t give much thought about how and where an animal lives until you begin to discuss the idea of ‘habitats’.

Can a camel live in a forest?

Are there sharks in our local pond?

And why is it that polar bears and penguins will never meet since they both live in icy places?

So many great questions 🙂

Today we’re sharing some AWESOME habitat projects, field trip ideas, FREE resources and affiliate links to some of our favorite learning items too. We’ll cover 7 different animal habitat locations using Cat in the Hat themed books from the Dr. Seuss non-fiction Learning Library series including more than 40 activities —

• live webcams,
• science experiments,
• field trip ideas,
• hands-on explorations and crafts!

This is a perfect topic to bring to life using cool science experiments, creative diorama crafts and some fun outings with the kids! Plus we’ve paired out favorite Cat in the Hat non-fiction books with awesome STEM activities for more fun ways to learn!

What are Animal Habitats?

Be sure to explain the following to your kids —

Definition:  a habitat is home or place where an animal lives, eats and finds all the things they need to survive.

There are a number of different habitats right around your own home.  For example, some animals call the woodland areas home and others live and thrive in a lake or pond habitat. 

Each of the books will share more details about both the environment, plants and animals in the habitat.  We’ve included some wonderful activities that can be paired with each of the books along with affiliate links for the books and other items we love that are related to the habitat projects.

Types of Animal Habitats

I know the first question kids will ask is “what types” or “how many habitats are there”?

There are a number of ways to classify habitats so this isn’t always an easy one to answer.

For example, many will say that the Earth’s largest habitat is the ocean — however, some scientists will tell you that there are different habitats in the ocean (tide pools, coral reefs, etc).

For kids, I would start with these different habitat areas:

1. Forests (coniferous & deciduous) — read our post on to learn about the two

2. Rainforests

3. Freshwater (like ponds, lakes and rivers)

6. Tundra (arctic and antarctic areas)

7. Grasslands (savanna, wild prairie lands)

DK has a really nice visual for the various types of land habitats – you can see they include ‘mountains’ as a habitat too.  After quite a bit of research, I’m not sure there’s any official list 🙂

Oh, and before I forgot to let you know, PBS television and the PBS website show some of the full videos and short clips that go with each of these books — be sure to check out The Cat in the Hat Knows a Lot about That if you’re interested.

Pond Animals & Activities

{Field Trip Idea} Visit a local pond! 

Use this free Day at the Pond printable for a list of fun things to do on your visit to a pond or lake. If there’s not one close to your home, try calling a local nature center or animal preserve to see if they have a pond habitat.

I love this DIY Underwater Magnifyer from One Time Through!  The perfect thing to take on a pond visit.

If you find tadpoles, Lovable Learning has details on how to raise tadpoles along with some great printables about a frog’s lifecycle.

Make your own Frog Pond Small World Play area — One Perfect Day

Print this free Lifecycle of a Frog lapbook — Worksheet Place

Create paper frogs, ducks & turtles — Ramblings of a Crazy Woman

Younger kids (ages 3 – 6) will enjoy this set of free pond printables too — from 1+1+1=1 blog

Kids will learn how animals stay warm, which pole is colder and a little about how climate change is affect the ice.  This is always a great topic as so many kids will never have the opportunity to experience these icy habitats.

Animal Life in Polar Regions

{Field Trip Idea}  Unless you live near the poles, this is a hard habitat to visit.  My suggestion would be to take a trip to the zoo and learn more about some of the animals that live at the poles.

The British Antarctic Survey also has a number of great Antarctic webcams that you can take a peek at to see the land and animals at the South Pole.

Get a peek at penguins in these cool penguin webcams & books !

Kids will enjoy this hands-on ‘ how do arctic animals stay warm’ science activity — Discover & Learn

Read & create with these Arctic Animals Book & Craft ideas !

You can also compare habitats with the fun Woodland vs. Arctic Habitat activity .

Plus here’s a list of more great books about polar animals!

Beach Habitat Crafts & Activities

{Field Trip Ideas}  Visit a local beach or shoreline to explore!

Try a few of these Fun Ways to Explore Science at the Beach on your next visit.

Learn about waves and beach erosion with this hands-on activity from Scientific American.

Explore these 25 Seashell Activities that encourage nature, art & science learning!

Make your own Ocean Discovery Bottle — The Imagination Tree

Enjoy a few of these Creative Beach craft & activities either while you play in the sand or after a beach day.

And here’s a wonderful Youtube video showing Life in a Tide Pool if you want to take a peek!

When someone says desert, the first thing that usually comes to mind is a cactus.  But there is so much more in the desert habitat.  Why Oh Why Are Deserts Dry?: All About Deserts introduces kids to these dry habitats all around the world.  Learn how such a large number of animals adapt to live in this habitat along with the variety of plants – not just the cactus – that call this place home.

Desert Habitat Project Ideas

{Field Trip Idea} This is another challenging habitat to explore since deserts are not close to many of us.  If you are close to a desert, than a visit would be fantastic – here’s a map of the world’s deserts !  If not, you might check to see if your local zoo has a desert exhibit. 

You can also call your local arboretum to see if they have any cactus in their exhibits.

Put together this free printable desert animals lapbook — Homeschool Share

To see the desert, visit the Sedona Red Rock webcam !

Learn more about desert adaptations with this fun online game .

Compare rainfall & see how dunes are made in this hands-on activity — Creative Tots

Craft your own Cactus Scene — Simply Crafty

And if you’re really adventurous, visit a local garden store and buy your own cactus for home!

I know this really isn’t a  ‘habitat’ persay but kids will be interested to know that there’s a whole different world that exists in the dark!  Out of Sight Till Tonight!: All About Nocturnal Animals shares about all the animals that wake up once we go to sleep.  I really like the number of adaptations that are shared about the animals who need to be able to see, hear and live in the dark.

Nocturnal Animals: Activities for Kids

While we know ‘nocturnal’ isn’t really a habitat per say, it’s still a specific environment & setting that many animals require for survival — and nighttime is very accessible to everyone so we thought is was important to include on our list!

{Field Trip Idea}   Just let the kids stay up late on night and explore your neighborhood!  We frequently take night walks and will see fireflies, racoons, fox and other nocturnal animals along with hearing owl cries too.  Your local nature center may also house some of these animals.

Go on a Nocturnal Scavenger Hunt with this free printable list!

Visit The Owl Channel to view webcams of different owls .

Print this free Nocturnal Animals lapbook to color! — Homeschool Share

I also love these Day & Night animal sorting cards from Spell Out Loud.

Visit Fun-a-Day for 24 great Owl Crafts & Activities !

You can also learn about ecolocation, bats, the moon & take a night hike too!

Rainforest Habitat Crafts & Activities

{Field Trip Idea}  Another challenging environment to visit (which is why I love that these books introduce these hard-to-explore habitats!)  Many zoos will have rainforest exhibits or house animals that live in the rainforest.  You might also see if your local pet store cares for exotic animals.

See what’s up in a real rainforest when you view these rainforest webcams from the World Land Trust

Learn more about this habitat with these 20 Amazing Rainforest Resources — Kid World Citizen

Create a Rainforest Diorama — Confessions of a Homeschooler

Take a Trip to the Rainforest with these virtual slideshows from The Rainforest Alliance

Make Your Own Rainforest in a Jar — Hike, Blog, Love

Enjoy these free printable Snake finger puppets — Mr. Printables

Or design your own Toucan Bird with this fun craft from I Heart Crafty Things

Ocean Habitat Project Ideas & Activities

{Field Trip Idea}  Visit your local aquarium and you’ll be introduced to tons of marine life!  Or spend a day at the ocean or near the sea — even visit a larger saltwater lake would be a great trip.

Create your own under-the-sea world with this Ocean Zones in a Bottle craft.

Play Ocean Bingo to learn more about the animals in various zones — Ellen McHenry’s Basement Workshop

Use this free printable Zones of the Ocean worksheet from Relentlessly Fun, Deceoptively Educational as you read the book!

Explore ocean waves and salt water with these great activities from the Gift of Curiosity

Try a few of these 30 ocean-themed crafts — A Night Owl Blog

And make your own model of the sea with this GORGEOUS Layers of the Ocean Zones project for kids !

More Cat in the Hat Science Activities:

If you missed the first part of our series, be sure to visit:

Part 1:  55 Science Activities with the Cat in the Hat.

Part 3:   Life Skills for Kids:  Money, Maps & Healthy Behaviors

More Animal Activities Kids will LOVE:

Zoo Scavenger Hunt & Wild Animal Activities

Learn about Animal Groups {free printable}

Ecology for Kids: Exploring Biomes

Why Do Scientists Experiment on Animals?

Animal studies in science are experiments that control an animal's behaviour or physiology for study, often to serve as a model for human biology where testing on humans is impractical or unethical.

The species or classification of animals used in testing largely depends on the goal of the experiment.

For example, zebrafish are quick to breed, easy to house, and transparent as embryos - but they also carry 70 percent of the genes found in humans. All this makes them suitable for studies on human disease and embryological development.

Rodents have a long history of being used for science experiments, and today make up around three quarters of all animal subjects in testing. Easy to raise and breed, their mammalian physiology and genomes overlap even more considerably with those of humans, making them suitable models for studying behaviours, toxicology, and the effects of medical treatments.

Non-human primates , especially chimpanzees and rhesus monkeys, have also been used extensively in scientific testing. While harder to reproduce in large numbers and challenging to house comfortably, experiments on our closest evolutionary relatives can yield valuable information on a wide range of issues, from drug toxicity to neurology.

However, the close likeness of non-human primates to ourselves also means their use in experiments is the most controversial of all types of animal testing. Generally, data across different countries, including the European Union , show that non-human primate research constitutes less than 1 percent of all animal studies.

However, studies on monkeys aren't yet phased out: In 2017, the US had a record-high number of studies involving monkeys.

How useful are animal models in experiments?

If conducted under strict methods with appropriate protocols, animal experimentation can provide reliable evidence on how that animal's physiology or behaviour responds under the experiment's conditions; genetic studies are particularly effective, while behavioural studies can yield less firm conclusions.

Unfortunately, the nature of experiments that make use of animal models can often lend themselves to being poorly designed, conducted, or analysed. There can also be a sex imbalance, with much of rodent research done only on male mice , for example.

Experiments that apply the findings to human biology require significant assumptions on whether any differences between them are significant. Even where animals are genetically altered to better reflect human biochemistry, there is always the risk that an unidentified behaviour or function might mean the experimental results can't be applied to humans.

This doesn't make animal models useless. As with all experiments, the weight of replicated experiments performed critically under peer review determines how confident we should be in a set of results.

It does mean we ought to be cautious about how results from an experiment based on an animal model might apply to our own bodies.

What are the ethics of testing on animals?

Concerns surrounding experiments using animal models are often based on the morality of depriving animals of their liberty or subjecting them to pain or discomfort, to meet a human need or value.

At an extreme end of the ethics spectrum is the claim that all animals have rights equal to humans, and therefore any experiment that wouldn't ethically be conducted on humans shouldn't be conducted on any animal.

Ethics boards today tend to weigh up the potential benefits of an experiment with the risks of harm and suffering to the animal. However, what constitutes a benefit , as well as objective ways to define acceptable limits of harm, pain, and discomfort in different animals can make this more challenging than first appears.

What is the future of animal testing?

More than half a century ago, zoologists William Russell and Rex Burch suggested experimentation should become more humane by following the three Rs; restrict when to use animals; refine the kinds of experiments conducted on them; and replace as the technology becomes available.

Advances in computer modelling and in-vitro tissue culture design are continuing to provide alternatives to animal models that don't suffer from the same ethical and practical limitations.

Human tissue models, such as those making up 3D tissue conglomerates called organoids , are increasingly serving as appropriate models for studying growth and development.

These solutions might not make the way we conduct the experiments themselves more trustworthy. But with robust debate and reliable review procedures, they will steadily make animal testing - and the ethical and practical problems they bring - a thing of the past.

All Explainers are determined by fact checkers to be correct and relevant at the time of publishing. Text and images may be altered, removed, or added to as an editorial decision to keep information current.

Arctic Animals – Science Experiment

I have animal lovers here who have been loving our science curriculum work right now, it’s about animals around the world. This week we learned more about arctic animals and this science experiment is perfect for learning more about them and how they stay warm.

Arctic Animals Science

*This post contains affiliate links, see my disclosure policy for more information.*

What child isn’t wondering how arctic animals stay warm considering we make our kids wear layers of clothing in the winter and the animals just have fur.

We learned how they live in the cold, how they survive the freezing cold temperatures. Considering my oldest likes to sit down and watch animal documentaries , she loves what we are learning right now. These are some amazing live cameras you can check out to take a virtual field trip to the Arctic .

Right now in our BookShark Science curriculum, we are learning animals from the Usborne World of Animals book .

My kids know what cold is, we don’t shy away from going out in the cold Saskatchewan winters. But arctic cold is something completely different. This is just one of many great arctic animals science experiments.

Arctic Animals – Science Experiment

This is a great arctic animal for kids activity to demonstrate how penguins, polar bears, and other arctic animals stay warm with their layers of fat we grabbed a package of Crisco (vegetable shortening) from the store and did up a bowl of icy cold water.

What You Need:

• latex gloves – optional

What we did to learn how animals stay warm in icy water:

We put a large bowl of water into the freezer to let it get extra cold and then added the ice cubes.

• We put a bare hand into the icy water first, just to get an idea of how cold the water really was.

The original plan was to wear latex gloves, but I couldn’t find them locally, so we simply stuck our hands inside a Ziploc sandwich bag. A bar of shortening is wrapped around the hand, and then another sandwich bag on covering the shortening, to keep it from falling off in the bowl.

It looks crazy but worked out great. And best of all for this Momma, clean-up was basically non-existent because we just had to slip our hand out of the baggie and toss the whole thing in the garbage. A win-win for me.

I didn’t want to cover my bare hand with shortening, and Gauge isn’t a fan of getting dirty, which is what you would be if you covered your hand with shortening.

Kids’ thoughts on this Arctic animals science experiment:

Gauge did think I was crazy when I was covering his hand in shortening, in a bag. And then asked him to put his hand in the water, though he wanted to do it first.

He loved it once he realized his hand wasn’t getting wet or cold. All three took turns doing this and said their hand stayed dry and warm.

This is a fun activity for kids. We did it in the middle of the winter, so it’s fresh in our minds just how cold it can be in the winter cold.

Wooden Polar Bears

Animal Education Learning Posters

Large Wooden Arctic Playset

Montessori Arctic Animals Wooden Matching Game

Learning About Animals in Your Homeschool

Learning About Animals – Animal Research

Animal Activities for Kids

Animal Science Curriculum for Homeschool

Arctic Animals Science Experiment

This week we learned more about arctic animals in the Arctic and Antarctic. We are going to experience just how these animals stay warm.

Instructions

• We put a large bowl of water into the freezer to let it get extra cold, and then added the ice cubes.
• The original plan was to wear latex gloves, but I couldn't find them locally, so we simply stuck our hand inside a Ziploc sandwich bag . A bar of shortening wrapped about the hand, and then another sandwich bag on covering the shortening, to keep it from falling off in the bowl.

Similar posts that you may be interested in:

Soil Testing Science for Gardening

Science YouTube Channels for Kids

Shark Buoyancy Science Experiment

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45 Fabulous 1st Grade Science Projects That Little Learners Will Love

Hands-on science is a surefire way to connect with every kid.

First graders will cheer when you announce that they’ll get to do a real, hands-on science experiment! These 1st grade science projects are easy for kids to do, with concepts that will help build their science knowledge for the future.

To make it even easier to find the best science projects for 1st graders, we’ve given each a rating based on difficulty and the materials you’ll need:

Difficulty:

• Easy: Low or no-prep experiments you can do pretty much anytime
• Medium: These take a little more setup or a longer time to complete
• Advanced: Experiments like these take a fairly big commitment of time and/or effort
• Basic: Simple items you probably already have around the house
• Medium: Items that you might not already have but are easy to get your hands on
• Advanced: These require specialized or more expensive supplies to complete

Animal and Nature Science Projects for 1st Grade

Weather science experiments for 1st grade.

• More 1st Grade Science Experiments and Projects

Learn about animals, plants, and the Earth in general with these fun science activities for first graders.

Sort animals by features

Difficulty: Easy / Materials: Basic

Use a printable or pull out the toy animals and have kids sort them into categories. It’s an early introduction to classification systems.

Learn more: Animal Sort at Fairy Poppins

Build the layers of Earth with Play-Doh

Another creative use for Play-Doh! Teach your students about the different layers of Earth and then have them create the layers using different colors of Play-Doh.

Learn more: Layers of the Earth at Evans Elementary School

Turn a plastic bag into a greenhouse

Difficulty: Easy / Materials: Medium

Turn your 1st grade science class into gardeners! Use a damp paper towel in a plastic bag to allow them to see a seed sprouting and growing roots.

Learn more: Greenhouse Bag at Lessons for Little Ones

See how shadows change throughout the day

Start in the morning: Have kids stand in one spot on the playground while a partner traces their shadow with sidewalk chalk . Ask them what they think will happen when they stand in the same spot during the afternoon, then head back outside after lunch to find out.

Learn more: Shadow Experiment at The First Grade Roundup

Discover how plants drink water

Capillary action is the name of the game, and your 1st grade science kiddos will be amazed at the results. Place celery stalks in cups of colored water, and watch as the leaves change color!

Learn more: Capillary Action at Lessons for Little Ones

Build a bird feeder

Set young engineers loose with wood craft sticks , glue, and string to create a bird feeder. Then research the best seeds to fill them with, and hang them outside your classroom window to draw in some feathered friends.

Learn more: DIY Bird Feeder at Mombrite

Observe the birds at your feeder

Once your feeder is in place, teach kids to identify common birds and keep track of their visits. Report their findings to one of Cornell Lab of Ornithology’s Citizen Science projects to let kids be part of real-life research. ( Find more bird-watching activities for kids here. )

Experiment with apples and oxidation

Apples turn brown when they’re cut open due to oxidation. Is there any way to prevent that from happening? This experiment aims to find out. ( Explore more apple activities here. )

Learn more: Apples Experiment at Camping Teacher

Use colorful beads to learn about camouflage

Animal camouflage is an important way for prey to protect itself from predators. To learn how effective it can be, place matching colored beads on top of a photo of wildflowers and see how long it takes students to find them all.

Learn more: Camouflage Activity at The First Grader Roundup

Expose a sponge fish to pollution

It’s never too early to start learning about how important it is to protect the Earth. Use sponge “fish” to see how polluted water affects the wildlife living in it.

Learn more: Pollution Experiment at The Owl Teacher

Dig in the dirt with claws

Animal adaptations allow creatures to live in just about every environment on Earth. Learn how claws help some animals survive and thrive by gluing plastic spoons to a glove.

Learn more: Claw Glove at The First Grade Roundup

Observe plant transpiration

Many plants take in more water than they need. What happens to the rest? Wrap a plastic bag around a living tree branch to see transpiration in action.

Learn more:  Leaf Transpiration at Teach Beside Me

Try these science activities to explore wind, rain, and more with your firsties.

Create a weather vane

A weather vane is one of the oldest scientific tools—they help people know which way the wind is blowing. Learn how to make your own with this cool weather experiment.

Learn more: Weathervane Experiment at Rookie Parenting

Grow a rainbow

Kids learn the colors of the rainbow along with chromatography as they watch marker streaks climb up and meet across a wet paper towel. The word might be a big one for little kids to learn, but they’ll love to see it in action!

Learn more: Grow a Rainbow at The Best Ideas for Kids

Make it rain

You need rain to make a rainbow. Simulate a rain cloud in a jar with shaving cream and food coloring, and see how the coloring saturates the “cloud” until it simply must fall.

Learn more: Shaving Cream Clouds at Fun Learning for Kids

Create frost in a can

This is an especially fun experiment during those chilly winter months. First, fill the can with ice and halfway with water. Then have the kids sprinkle salt in the can and cover the top. Finally, shake it and wait about three minutes for the frost to begin to appear.

Learn more: Frost in a Can at Kindergarten Worksheets and Games

Create an avalanche

Learn about the destructive power of an avalanche in a safe way with this experiment. All you need is flour, cornmeal, pebbles, and a plastic tray.

Learn more: Avalanche Experiment at A Dab of Glue Will Do

More 1st Grade Science Projects and Experiments

Looking for more? Try these experiments on a range of subjects to teach first graders all about science.

Give gummy bears a bath

Drop gummy bears into different liquid solutions to see how they change (or don’t) over time. Kids will learn about osmosis, as well as how scientists must be good observers.

Learn more: Gummy Bear Experiment at First Grade Buddies + Co

Play a DIY flute

These homemade flutes are fun to play, but they also help young kids learn about sound. Let them experiment with straw lengths to see what tones they can make.

Learn more: DIY Pan Flute at Buggy and Buddy

Play with Play-Doh to learn why we have bones

Ask kids to build a person from Play-Doh and see if it will stand on its own. Then show them how adding drinking straws gives it structure and strength, and explain that bones do the same for us! ( Get more clever ways to use Play-Doh in the classroom here. )

Learn more: Play-Doh Bones at Keeping My Kiddo Busy

Find out which objects magnets attract

Equip students with magnets and send them out to explore and discover which objects the magnet will stick to and which won’t. Record their findings on the free printable worksheet at the link.

Learn more: Magnet Science Experiments at Fairy Poppins

Grow a crystal garden

First grade science students might not grasp the concept of supersaturated solutions, but they’ll still love a good crystal project! Grab some magnifying glasses and let them examine the crystals up close (try not to touch, as they’re very fragile) to see the cool geometric structures.

Learn more: Crystal Garden at Babble Dabble Do

Build a jelly bean structure

If you’re doing this STEM project in the spring, jelly beans make the perfect base. If you can’t get a hold of jelly beans, try substituting tiny marshmallows in their place. Make sure to have some extras on hand since little hands are likely to snack as they build.

Learn more: Jellybean STEM Challenge at The STEM Laboratory

Experiment with marshmallow Peeps

Peeps used to just be an Easter treat, but these days you can find them in different shapes throughout much of the year. Use them to practice making predictions and recording observations with this sweet experiment.

Learn more: Easter Peeps Science Experiment at Gift of Curiosity

Spark excitement with static electricity

No doubt your 1st grade science students have already encountered static electricity by rubbing a balloon on their hair. This experiment takes things a step further, letting kids explore which objects an electrically charged balloon can pick up and which it can’t.

Learn more: Static Electricity Balloons at Kids Activities Blog

Melt crayons to explore solids and liquids

Dig out some old crayons and use them for this easy experiment that demonstrates the difference between liquids and solids. When you’re done, you’ll have a cool piece of art to display. ( Discover more uses for broken crayons here. )

Learn more: Melting Crayons at First Grade Circle

Talk through a paper cup phone

This classic experiment will help your 1st grade science class understand that sound travels in waves, through the air, and across other objects. Watching their faces light up when they hear whispers in their cups will make your day!

Learn more: Paper Cup Phone at There’s Just One Mommy

Blow a bubble snake

Difficulty: Medium / Materials: Basic

You’ll need to plan this experiment for a day with nice weather since it is best suited to outdoors. You will need an empty water bottle, a washcloth, a rubber band, a small bowl or plate, food coloring, scissors or box cutters, distilled water, dish soap, and Karo syrup or glycerin. There’s a lot of prep, but the end result is definitely worth it!

Learn more: Bubble Snakes at Hand 2 Mind

Learn why we have night and day

The Earth’s daily rotation gives us days and nights. This simple demo helps kids understand that. They draw a day scene and a night scene on a paper plate, then cover it with half of another plate that can be moved. This is an art project and 1st grade science experiment all rolled into one.

Learn more: Night and Day Play at Universe Awareness

Float food coloring on milk

Learn about surface tension by dropping food coloring onto different types of milk (whole, skim, cream, etc.). Then use dish soap to break down the fats and surface tension, and watch the colors dance!

Learn more: Surface Tension Milk at STEAMsational

Drop water onto a penny

Continue your exploration of surface tension by adding water drop-by-drop to a penny. The surface tension will allow you to add far more water than you might think.

Will it sink or swim?

Ask students to predict whether a variety of items will sink or float in water. Then test each object to check their hypotheses.

Blow up a balloon using yeast

This is similar to the classic lemon juice and baking soda experiment many kids do at some point. But this one is better for younger kids since you don’t have to worry about them splashing the juice in their eyes. Kids will be just as astonished at the results as the yeast eats the sugar and produces carbon dioxide gas!

Learn more: Yeast Balloon Experiment at The STEM Laboratory

Push on air

Teach your students about air compression and air pressure using a barrel, plunger, syringe, and flexible tube. Kids will definitely get a kick out of air wrestling and popping off their plungers using air pressure.

Learn more: Air Pressure Experiment at First Grade Adventurers

Test your reaction time

Do your students have lightning-quick reflexes? Find out with this easy experiment. One student holds a ruler vertically, while another places their hand just beneath and waits. When the first student drops the ruler, the second catches it as quickly as possible, seeing how many inches passed through their fingers first.

Learn more: Reaction Time Experiment at Science Sparks

Assemble a DIY lava lamp

Your firsties are too young to remember the lava lamp craze, but this science project will give them a taste of it as they learn about liquid density.

Learn more: Salt Volcano Lava Lamp at What Do We Do All Day?

Learn the scientific method with candy

See the scientific method in action as kids hypothesize what will happen to various types of candy in the hot sun. Observe, record, and analyze your results to see if their predictions were correct.

Learn more: Candy Scientific Method Activity at Playdough to Plato

Look into mirrors to discover symmetry

By now, 1st grade science students may have noticed that mirrors reflect objects backward. Ask them to write the alphabet in capital letters, then hold it up to the mirror. Which letters are the same when they’re reflected? Use those findings to talk about symmetry.

Learn more: Mirror Symmetry at Buggy and Buddy

Create a super-simple circuit

This is the perfect way to introduce the concept of electricity to young students since the materials and steps are minimal. You will need a D battery, tinfoil, electrical tape, and a light bulb from a flashlight.

Learn more: Super-Simple Circuit at What Do We Do All Day?

“Bend” a pencil using light refraction

Tell your students you’re going to bend a pencil without touching it. Drop it into a glass of water and have them look at it from the side. Light refraction makes it appear to be in two pieces!

Learn more: Bending Pencil Experiment at STEAMsational

Roll marbles to explore momentum

Momentum is “mass in motion,” but what does that really mean? Find out by rolling marbles of different sizes down rulers placed at various slopes.

Learn more: Momentum Experiments at Frugal Fun for Boys and Girls

Dunk eggs to understand dental health

Grown-ups are always telling kids sugary drinks are bad for their teeth, so try this experiment to put your money where your mouth is! Eggshells are a good substitute for teeth since they’re both made of calcium. Leave eggs in different kinds of beverages to see which ones do the most damage to the shells.

Learn more: Dental Health Eggs at First Grade Funtastic

Melt ice cubes to make new colors

Color mixing is one of those incredibly cool activities that kids will want to try again and again. Make ice cubes using primary colors, then let them melt together to see what new colors you can create.

Learn more: Color Mixing at Gift of Curiosity

Fly a paper airplane

Kid absolutely love creating and flying paper airplanes, so this experiment is sure to be a hit. Have your students create different-style planes and then experiment with thrust and lift to see which fly the farthest, highest, etc.

Weigh items with a homemade balance scale

Make a simple balance scale with a coat hanger, yarn, and some plastic cups. Have students gather items from around the classroom, make predictions about which will be heavier, then test their hypothesis.

Learn more: DIY Balance Scale at WikiHow

If you love 1st grade science, be sure to check out these 25 First Grade STEM Challenges .

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40 Easy Kindergarten Science Experiments for Hands-On Learning

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• Published: 24 September 2021

On the past, present, and future of in vivo science

• Ellen P. Neff 1  

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Animal testing and experiments FAQ

How many animals are used in experiments each year?

Which animals are used in experiments, what kinds of experiments are animals used in, what kinds of institutions use animals in experiments, where do laboratories get the animals they use in experiments, what is life like for animals in laboratories, what happens to the animals once an experiment is over, aren’t there laws to protect animals used in experiments, why are animals still used in experiments, what are the alternatives to experiments on animals, what are the advantages of using non-animal alternatives instead of animals in experiments.

• What are you doing to end experiments on animals?

What can I do to help animals in laboratories?

Stand with us to demand that the federal government, state governments, companies and universities stop relying on outdated animal experiments.

It is estimated that more than 50 million animals are used in experiments each year in the United States. Unfortunately, no accurate figures are available to determine precisely how many animals are used in experiments in the U.S. or worldwide.

The U.S. Department of Agriculture (USDA) does compile annual statistics on some animals used in experiments, including cats , dogs , guinea pigs , hamsters , pigs , primates , rabbits and  sheep .

However, the animals most commonly used in experiments—“purpose-bred” mice and rats  (mice and rats bred specifically to be used in experiments)—are not counted in annual USDA statistics and are not afforded the minimal protections provided by the Animal Welfare Act. The Animal Welfare Act is a federal law that sets minimal standards for the treatment of certain warm-blooded animals used in experiments. The law also requires that unannounced inspections of all regulated animal testing facilities are carried out annually, although some facilities only receive partial inspections . In addition to purpose-bred mice and rats, animals such as crabs, fish , frogs, octopuses and turtles , as well as purpose-bred birds , are not covered by the Animal Welfare Act. The failure to protect these animals under the law means that there is no oversight or scrutiny of their treatment in the laboratory or the experiments performed on them. And, because these animals are not counted, no one knows how many of them are suffering in laboratories. It also means that facilities using unprotected species in experiments are not required to search for alternative, non-animal methods that could be used to replace or reduce harmful experiments that use animals.

View Animals Used in Experiments by State

View Dogs Used in Experiments by State

Read Dogs Used in Experiments FAQ

Use our Animal Laboratory Search Tool  to find information about universities, hospitals, companies and other organizations that use certain animals in experiments

View a list of U.S. laboratories that use certain animals in experiments ; click on “License Type” and select “Class R – Research Facilities." Note that numbers only include animals covered by the Animal Welfare Act.

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Animals used in experiments include baboons, cats , cows , dogs , ferrets,  fish , frogs, guinea pigs , hamsters , horses , llamas, mice , monkeys (such as marmosets and macaques), owls, pigs , quail, rabbits , rats and  sheep .

Chimpanzees have thankfully not been subjected to invasive experiments in the U.S. since 2015, when federal decisions were made to prevent their use. Despite this, hundreds of chimpanzees are still languishing in laboratories while they wait to be moved to sanctuaries.

Animals are used in many different kinds of experiments. These are just a few examples:

• Dogs have their hearts, lungs or kidneys deliberately damaged or removed to study how experimental substances might affect human organ function.  
• Monkeys are taken from their mothers as infants to study how extreme stress might affect human behavior.
• Mice are force-fed daily doses of a chemical for two years to see if it might cause cancer in humans.
• Cats have their spinal cords damaged and are forced to run on treadmills to study how nerve activity might affect human limb movement.
• Ferrets are deliberately infected with extremely painful, potentially fatal diseases (such as RSV, COVID-19 or Ebola) and not given pain relief or treatment before their death to study how humans might be affected by the same disease.  
• Pigs are implanted with various devices (such as pacemakers and dental implants) to study how human bodies might respond to such devices.  
• Pregnant rabbits are force-fed toxic pesticides every day for several weeks to study how human mothers and babies might be affected if they were exposed to the pesticides.
• Sheep are subjected to high pressures (such as those experienced deep underwater) for hours at a time and then returned to normal pressure so that their response can be observed.
• Rats are placed in small tubes and are forced to inhale cigarette smoke for hours at a time to study how humans might respond to cigarette smoke.   
• Baboons are injected with endometrial tissue to induce painful symptoms of endometriosis and study how humans might be affected by the disorder.
• Horses are infected with a potentially fatal virus (such as hepatitis) and their symptoms monitored to study how humans might be affected by the same virus.

Experiments are often excruciatingly painful for the animals used and can vary in duration from days to months to years. The experiment can cause vomiting, diarrhea, irritation, rashes, bleeding, loss of appetite, weight loss, convulsions, respiratory distress, salivation, paralysis, lethargy, bleeding, organ abnormalities, tumors, heart failure, liver disease, cancer and death.

There is no limit to the extent of pain and suffering that can be inflicted on animals during experiments. In some instances, animals are not given any kind of pain medication to help relieve their suffering or distress during or after the experiment on the basis that it could affect the experiment.

Animals are typically killed once an experiment is over so that their tissues and organs can be examined, although it is not unusual for animals to be used in multiple experiments over many years. There are no accurate statistics available on how many animals are killed in laboratories every year.

Read Cosmetics Animal Testing FAQ

• Read about our 2022 undercover investigation at Indiana laboratory Inotiv, one of America’s largest animal testing labs. We documented hundreds of dogs, monkeys, rats and pigs undergoing experiments, including terrified beagle puppies being force-fed a potentially toxic drug in cruel and ineffective months-long tests paid for by Crinetics, a pharmaceutical company in San Diego.
• Read about our 2019 undercover investigation at a Michigan laboratory where thousands of dogs are killed every year. After weeks of pressure from the public, the pesticide company that had commissioned a year-long fungicide test on 32 dogs agreed that the test was unnecessary and released the dogs to one of our shelter partners to be adopted.

Chemical, pesticide and drug companies (as well as contract laboratories that carry out tests for those companies), public and private universities, community and technical schools, government facilities, Veterans Affairs (VA) facilities and hospitals all use animals in experiments.

View USDA List of Organizations that Use Dogs in Experiments

View Chart of Institutions That Use Dogs in Experiments

The majority of animals in laboratories are “purpose-bred” meaning that they are bred specifically to be used in experiments. People who breed and sell certain purpose-bred animals are called Class A dealers and are licensed and inspected by the U.S. Department of Agriculture (USDA). Facilities that only sell purpose-bred mice, rats, birds or cold-blooded animals such as crabs, fish, frogs, octopuses and turtles to laboratories are excluded and are not licensed or inspected by the USDA.

Some animals used in experiments are taken from the wild—including birds and  monkeys . 

Historically, some cats and dogs  were sold to laboratories by brokers known as random source Class B dealers, who acquired animals at auctions, from newspaper ads and various other sources, including animal shelters. Random source Class B dealers have not been allowed to operate since 2015 when Congress first passed legislation to prevent them from being licensed.  

Some cats and dogs in laboratories are still obtained directly from animal shelters, a practice known as “pound seizure.” Pound seizure laws vary from state to state with one state (Oklahoma) requiring shelters to give cats and dogs to laboratories, rather than euthanizing them, and others allowing or prohibiting laboratories from taking animals from animal shelters. Some states have no laws at all, leaving it up to the individual shelter or locality.

View Pound Seizure Laws by State

Animals in laboratories suffer immensely. In addition to the painful experiments that the vast majority of animals in laboratories experience over days, months, years or even decades, life in a laboratory is typically a miserable and terrifying experience.

Typically kept alone in barren steel cages with little room to move around and few, if any, comforts, such as toys or soft bedding, animals often become excruciatingly lonelyand anxious, often devoid of the companionship of other animals or the loving touch of a human. Animals in laboratories can associate humans with painful situations and, with no way to hide or get away, they panic whenever a person approaches their cage or freeze with fear when they are taken into treatment rooms. Despite this, dogswill often still seek out human attention.

Animals in laboratories typically also have to watch (or hear) other animals suffering, including their own parents, siblings or babies. High levels of constant stress can cause animals to exhibit unnatural behaviors. For example, it is not uncommon for monkeys to mutilate themselves or to rock or vocalize constantly as a way to help relieve their anxiety, mice to overgroom each other until they are completely bald, and dogs to continually pace.  

Very often the experiments themselves lead to suffering and death. In our 2022 undercover investigation we documented monkeys in “restraint chairs”—devices that are used to hold monkeys in place while the experiments are carried out—who accidentally hanged themselves while unattended. We also documented a dog named Riley used to test a substance so toxic that it brought him near death after only two days of forced dosing. He was hypersalivating, trembling, vomiting, and moaning, yet was dosed yet again with this highly toxic substance. Later, he lay on the floor, unable to stand. Our undercover investigator tried to comfort him while he was dying, but Riley was left to suffer in excruciating pain overnight because the laboratory’s veterinarian was unavailable on a weekend

Animals in laboratories are also subject to mistreatment by inexperienced or careless staff. Although there are penalties for laboratories when animals are injured or killed due to negligence or when they fail to meet minimum standards of animal care, in reality, the fines are typically either very small or waived entirely.

In some cases, animals die as a deliberate result of the experiment. For example, the LD50 (lethal dose 50%) test, which is typically performed on mice, rats, pigeons, quail and fish, involves determining the dose of a substance (such as a pesticide) that kills (or would lead to the death of) 50% of the animals tested.

It is extremely rare that animals are either adopted out or placed into a sanctuary after research is conducted on them. However, more and more states are passing laws that require laboratories, when possible, to offer dogs and cats to shelters and other rescue organizations so they can be adopted into loving homes after the experiments they were used in have ended. As of December 2023, 16 states have such laws.

The Animal Welfare Act was designed to protect certain animals, like dogs and monkeys, used in experiments, but the law only offers minimal standards for housing, food and exercise. The Animal Welfare Act also stipulates that the proposed experiments be reviewed by an Institutional Animal Care and Use Committee, whose members are appointed by the laboratory itself and largely made up of employees of the institution. A 2014 audit report reviewing Animal Welfare Act oversight of laboratories found that “animals are not always receiving basic humane care and treatment and, in some cases, pain and distress are not minimized during and after experimental procedures.”

The animals most commonly used in experiments—“purpose-bred” mice and rats  (mice and rats bred specifically to be used in experiments)—are not counted in annual USDA statistics and are not afforded the minimal protections provided under the Animal Welfare Act. The Animal Welfare Act is a federal law that sets minimal standards for the treatment of certain warm-blooded animals used in experiments. The law also requires that unannounced inspections of all regulated research facilities are carried out annually. In addition to purpose-bred mice and rats, animals such as crabs, fish , frogs, octopuses and turtles as well as purpose-bred birds are not covered by the Animal Welfare Act. The failure to protect these animals under the law means that there is no oversight or scrutiny of their treatment and use in the laboratory. And, because these animals are not counted, no one knows how many of them are suffering in laboratories. It also means that facilities using unprotected species in experiments are not required to search for alternative, non-animal methods that could be used to replace or reduce harmful experiments that use animals.

The vast majority of experiments on animals are not required by government law or regulations. Despite that, government agencies often seem to prefer that companies carry out animal tests to assess the toxicity or efficacy of products such as industrial chemicals, pesticides, medical devices and medicines.

For example, the Environmental Protection Agency (EPA) requires that a new pesticide be fed to dogs for 90 days as part of its evaluation and approval process. The Food and Drug Administration (FDA), which regulates various products such as drugs, medical devices, food, fragrances and color additives, will not approve potential drugs unless they are first tested on animals, which usually includes dogs. In addition to tests on  dogs ,  mice and rats ,  rabbits ,  birds  and primates are also used to test pesticides and drugs. These types of tests have been performed for years, regardless of whether they provide valuable information. While some regulatory agencies, like the EPA, are now taking a critical look at these animal tests to determine if they provide information necessary for assessing how safe a product or substance is for humans, and if better approaches are available, others have done little. More efforts can be made by agencies to invest in and encourage the development of non-animal methods.

Swapping animal experiments for non-animal alternative methods seems like a straightforward process, given that using animals has so many limitations and sophisticated new technologies offer countless possibilities for creating methods that are more humane and that more accurately mimic how the human body will respond to drugs, chemicals or treatments. Unfortunately, developing these alternatives is a complex process facing many obstacles, including inadequate funding. In most cases, a non-animal alternative must be formally validated—historically an expensive and lengthy process—in order to be accepted by government regulatory agencies, both in the U.S. and globally, although new, faster approaches to approving these methods are being developed. In contrast, animal experiments have never been subjected to the same level of scrutiny and validation. Despite these challenges, many scientists are increasingly committed to developing and using non-animal methods.

The world is continuously moving toward a future dominated by sophisticated methods that use human cells, tissues and organs, 3D printing, robotics, computer models and other technologies to create experiments that do not rely on animals.

While many animal experiments have not changed since they were developed decades ago and will always have severe limitations, advanced non-animal methods represent the very latest techniques that science has to offer, provide countless possibilities to improve our understanding and treatment of human diseases and will only continue to improve over time. Non-animal methods also have several advantages over outdated animal experiments: they more closely mimic how the human body responds to drugs, chemicals and treatments; they are more efficient and often less expensive; and they are more humane. Ultimately, moving away from animal experiments is better for both humans and animals.

We advocate for the immediate replacement of animal experiments with available non-animal methods and for more funding to develop new non-animal methods. A concerted effort to shift funding and technological development toward more non-animal alternatives will lead us to a future where animal experiments are a thing of the past.

Examples of non-animal alternative methods

• “Organs-on-chips” are tiny 3D chips created from human cells that look and function like miniature human organs. Organs-on-chips are used to determine how human systems respond to different drugs or chemicals and to find out exactly what happens during infection or disease. Several organs, representing heart, liver, lungs or kidneys, for example, can be linked together through a “microfluidic” circulatory system to create an integrated “human-on-a-chip” model that lets researchers assess multi-organ responses.
• Sophisticated computer models use existing information (instead of carrying out more animal tests) to predict how a medicine or chemical, such as drain cleaner or lawn fertilizer, might affect a human.
• Cells from a cancer patient’s tumor are used to test different drugs and dosages to get exactly the right treatment for that specific individual, rather than testing the drugs on animals.
• Specialized computers use human cells to print 3D tissues that are used to test drugs.
• Skin cells from patients, such as those with Alzheimer’s disease, are turned into other types of cells (brain, heart, lung, etc.) in the laboratory and used to test new treatments.
• Sophisticated computer programming, combined with 3D imaging, is used to develop highly accurate 3D models of human organs, such as the heart. Researchers then input real-world data from healthy people and those with heart disease to make the model hearts “beat” and test how they might respond to new drugs.

Human cells or synthetic alternatives can replace horseshoe crab blood in tests to determine whether bacterial contaminants are present in vaccines or injectable drugs.

• Animal experiments are time-consuming and expensive.
• Animal experiments don’t accurately mimic how the human body and human diseases respond to drugs, chemicals or treatments.
• Animals are very different from humans and, therefore, react differently.
• Increasing numbers of people find animal testing unethical.
• There are many diseases that humans get that animals do not.

What are you doing to end experiments on animals?

We advocate for replacing animals with non-animal alternative methods when they are available and more funding for the development of new alternative methods to quickly replace antiquated and unreliable animal tests and experiments. Our two main areas of focus are ending cosmetics animal testing  and ending experiments on dogs .

Cosmetics testing on animals

We—along with our partner, Humane Society International —are committed to ending cosmetics animal testing forever. Through our  Be Cruelty-Free campaign, we are working in the United States and around the globe to create a world where animals no longer have to suffer to produce lipstick and shampoo. 

• In the United States, we are working to pass the Humane Cosmetics Act , federal legislation that would prohibit animal testing for cosmetics, as well as the sale of animal-tested cosmetics.
• We are also working in several U.S. states to pass legislation that would end cosmetics animal testing. As of March 2024, 12 states (California, Hawai'i, Illinois, Louisiana, Maine, Maryland, Nevada, New Jersey, New York, Oregon, Virginia and Washington) have passed laws banning the sale of animal-tested cosmetics.
• Internationally, as of December 2023, 45 countries have passed laws or regulations to ban cosmetics animal testing, including every country in the European Union, Australia, Brazil, Canada, Chile, Colombia, Ecuador, Guatemala, Iceland, India, Israel, Mexico, New Zealand, Norway, South Korea, Switzerland, Taiwan, Turkey, the United Kingdom.
• We work with scientists from universities, private companies and government agencies around the globe to promote the development, use and regulatory acceptance of non-animal test methods that will reach beyond cosmetics.
• We educate consumers about animals used in cruel and unnecessary cosmetics tests and how to shop for cruelty-free cosmetics and personal care products.

Experiments on dogs

There is no place for harmful experiments on dogs in the U.S. We are committed to ending this practice.

• In the summer of 2022, we led the removal of 3,776 beagles from Envigo, a facility in Virginia that bred dogs to sell to animal laboratories. This historic mission was the result of a lawsuit filed by the U.S. Department of Justice that described shocking violations of the Animal Welfare Act at the facility. Instead of continuing to suffer, the dogs were removed from Envigo and headed to loving homes , a process facilitated by our shelter and rescue partners around the country.
• In April 2022, we released the results of our undercover investigation at Inotiv, an Indiana laboratory where thousands of dogs, monkeys, pigs and rats are used in experiments and killed.
• In 2021, we released a report examining the U.S. government’s role in using dogs in experiments. We found that the government uses millions of taxpayer dollars to fund harmful experiments on dogs each year—and also seems to prefer that companies carry out dog tests. Our researchers scrutinized public records and found that between 2015 and 2019, the National Institutes of Health (NIH) awarded more than $200 million to 200 institutions for 303 projects that used dogs in harmful experiments. Dogs were subjected to multiple surgeries, fitted with equipment to impair their heart function and implanted with devices to alter normal bodily functions. Following the conclusion of an experiment, dogs are typically killed instead of being adopted into loving homes.
• In 2019, we released the results of our undercover investigation at a Michigan laboratory where thousands of dogs are killed every year. After weeks of pressure from the public, the pesticide company that had commissioned a test year-long fungicide test on 32 dogs, agreed that the test was unnecessary and released the dogs to one of our shelter partners so they could be adopted.
• After a recent analysis we performed that showed the 90-day dog test for pesticide registration was rarely used by the Environmental Protection Agency (EPA) to assess the risk that pesticides pose to humans, we are urging the agency to eliminate or significantly limit this test in the near future. We also want the agency to reaffirm their previously stated commitment to end their reliance on using mammals to test pesticides and chemicals by 2035.
• We are asking the Food and Drug Administration (FDA) to support the development of alternative methods that replace dogs in experiments. 
• We want the Department of Veterans Affairs (VA) to adopt the recommendations of an independent panel review released in 2020 that analyzed VA experiments using dogs, identified several areas where dogs are not needed and urged the agency to develop a strategy to replace all animal use. 
• We are recommending that the National Institutes of Health (NIH) scrutinize grant proposals for projects using dogs, by applying strict criteria that must be met before dogs can be used and that they ban the use of dogs in experiments that cause unrelieved pain. We are also requesting that the NIH define a date when they will no longer fund or support experiments on dogs.
• prohibit or limit the use of dogs in experiments not required by federal law, similar to laws passed in California and Illinois .
• ensure an opportunity for  dogs and cats to be adopted into loving homes after the experiment ends.
• strengthen regulatory oversight of facilities that breed dogs destined for laboratories and increase penalties for animal welfare violations.
• Direct state funding to support the research and development of modern non-animal technologies, similar to the law passed in Maryland .

One easy way to help animals suffering in cosmetics tests is to swap out your personal care and household products for cruelty-free versions! Cosmetics (such as shampoo, deodorant and lipstick) and household products (such as dish soap, laundry detergent and glass cleaner) are typically tested on guinea pigs , rabbits ,  mice and rats .

Help us demand better for animals used in experiments through the following actions:

• Tell the FDA to stop encouraging companies to test on animals and instead switch to sophisticated non-animal alternatives.
• Stand with us to end research and tests on dogs by signing our petition.
• Urge the USDA to do their job and help protect animals in laboratories.
• Ask your federal legislators in Congress to ban cosmetic tests on animals.
• Support efforts to replace animal experiments with advanced non-animal alternatives that are better for both human health and animal welfare.

Follow us on Facebook to learn the latest news and actions related to animals in laboratories!

Alternatives to horseshoe crab blood

The Humane Society of the United States urges that horseshoe crab blood be replaced with non-animal methods when conducting endotoxin tests for medical products.

Vaccine, injectable drug and medical device manufacturers must test for endotoxins, a type of bacterial contaminant that, if present, can cause patients to develop symptoms that can include fever, chills, headache and nausea. Blood from horseshoe crabs is used to conduct the Limulus amebocyte lysate (or LAL) test for endotoxins.

The problem

To create this test, horseshoe crabs are captured from the wild and up to 30% of their blood is removed by medical supply companies. The crabs are later returned to the wild; however, it is estimated that 10-15% or more of them die as a result of this process.

In addition to being collected for their blood, horseshoe crabs are gathered up by fisheries, which use them as bait. These practices have led to a rapid decrease in the horseshoe crab population, putting them at risk of extinction. The decrease in wild horseshoe crab populations also impacts other species, including migratory shorebirds like the red knot, a threatened species that depends on horseshoe crab eggs for food.

THE solution

Scientists have developed recombinant Factor C (rFC), a synthetic alternative to the protein in horseshoe crab blood that can detect bacterial endotoxins. Repeated studies have demonstrated that rFC is equivalent or superior to the LAL test. A second method—the monocyte activation test—uses human cells and can not only detect bacterial endotoxins, but also pyrogenic (fever-causing) non-endotoxins.

what should be done

As a member of the Horseshoe Crab Recovery Coalition, the Humane Society of the United States is advocating for the replacement of the Limulus amebocyte lysate test with recombinant Factor C (rFC) or the monocyte activation test (MAT).

We urge the U.S. Pharmacopoeia—which sets quality, purity, strength and identity standards for medicines, food ingredients and dietary supplements—to encourage manufacturers to use rFC or MAT rather than LAL.

We also urge the U.S. Food and Drug Administration to update its guidance for vaccine, injectable drug and device manufacturers to indicate that these non-animal tests are now the preferred methods for endotoxin and pyrogenicity testing.

Please consider a generous gift to help spare dogs from life in a lab and give all animals a better life!

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• J Prev Med Hyg
• v.63(2 Suppl 3); 2022 Jun

Ethical considerations regarding animal experimentation

Aysha karim kiani.

1 Allama Iqbal Open University, Islamabad, Pakistan

2 MAGI EUREGIO, Bolzano, Italy

DEREK PHEBY

3 Society and Health, Buckinghamshire New University, High Wycombe, UK

GARY HENEHAN

4 School of Food Science and Environmental Health, Technological University of Dublin, Dublin, Ireland

RICHARD BROWN

5 Department of Psychology and Neuroscience, Dalhousie University, Halifax, Nova Scotia, Canada

PAUL SIEVING

6 Department of Ophthalmology, Center for Ocular Regenerative Therapy, School of Medicine, University of California at Davis, Sacramento, CA, USA

PETER SYKORA

7 Department of Philosophy and Applied Philosophy, University of St. Cyril and Methodius, Trnava, Slovakia

ROBERT MARKS

8 Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva, Israel

BENEDETTO FALSINI

9 Institute of Ophthalmology, Università Cattolica del Sacro Cuore, Fondazione Policlinico Universitario A. Gemelli-IRCCS, Rome, Italy

NATALE CAPODICASA

10 MAGI BALKANS, Tirana, Albania

STANISLAV MIERTUS

11 Department of Biotechnology, University of SS. Cyril and Methodius, Trnava, Slovakia

12 International Centre for Applied Research and Sustainable Technology, Bratislava, Slovakia

LORENZO LORUSSO

13 UOC Neurology and Stroke Unit, ASST Lecco, Merate, Italy

DANIELE DONDOSSOLA

14 Center for Preclincal Research and General and Liver Transplant Surgery Unit, Fondazione IRCCS Ca‘ Granda Ospedale Maggiore Policlinico, Milan, Italy

15 Department of Pathophysiology and Transplantation, Università degli Studi di Milano, Milan, Italy

GIANLUCA MARTINO TARTAGLIA

16 Department of Biomedical, Surgical and Dental Sciences, Università degli Studi di Milano, Milan, Italy

17 UOC Maxillo-Facial Surgery and Dentistry, Fondazione IRCCS Ca Granda, Ospedale Maggiore Policlinico, Milan, Italy

MAHMUT CERKEZ ERGOREN

18 Department of Medical Genetics, Faculty of Medicine, Near East University, Nicosia, Cyprus

MUNIS DUNDAR

19 Department of Medical Genetics, Erciyes University Medical Faculty, Kayseri, Turkey

SANDRO MICHELINI

20 Vascular Diagnostics and Rehabilitation Service, Marino Hospital, ASL Roma 6, Marino, Italy

DANIELE MALACARNE

21 MAGI’S LAB, Rovereto (TN), Italy

GABRIELE BONETTI

Astrit dautaj, kevin donato, maria chiara medori, tommaso beccari.

22 Department of Pharmaceutical Sciences, University of Perugia, Perugia, Italy

MICHELE SAMAJA

23 MAGI GROUP, San Felice del Benaco (BS), Italy

STEPHEN THADDEUS CONNELLY

24 San Francisco Veterans Affairs Health Care System, University of California, San Francisco, CA, USA

DONALD MARTIN

25 Univ. Grenoble Alpes, CNRS, Grenoble INP, TIMC-IMAG, SyNaBi, Grenoble, France

ASSUNTA MORRESI

26 Department of Chemistry, Biology and Biotechnology, University of Perugia, Perugia, Italy

ARIOLA BACU

27 Department of Biotechnology, University of Tirana, Tirana, Albania

KAREN L. HERBST

28 Total Lipedema Care, Beverly Hills California and Tucson Arizona, USA

MYKHAYLO KAPUSTIN

29 Federation of the Jewish Communities of Slovakia

LIBORIO STUPPIA

30 Department of Psychological, Health and Territorial Sciences, School of Medicine and Health Sciences, University "G. d'Annunzio", Chieti, Italy

LUDOVICA LUMER

31 Department of Anatomy and Developmental Biology, University College London, London, UK

GIAMPIETRO FARRONATO

Matteo bertelli.

32 MAGISNAT, Peachtree Corners (GA), USA

Animal experimentation is widely used around the world for the identification of the root causes of various diseases in humans and animals and for exploring treatment options. Among the several animal species, rats, mice and purpose-bred birds comprise almost 90% of the animals that are used for research purpose. However, growing awareness of the sentience of animals and their experience of pain and suffering has led to strong opposition to animal research among many scientists and the general public. In addition, the usefulness of extrapolating animal data to humans has been questioned. This has led to Ethical Committees’ adoption of the ‘four Rs’ principles (Reduction, Refinement, Replacement and Responsibility) as a guide when making decisions regarding animal experimentation. Some of the essential considerations for humane animal experimentation are presented in this review along with the requirement for investigator training. Due to the ethical issues surrounding the use of animals in experimentation, their use is declining in those research areas where alternative in vitro or in silico methods are available. However, so far it has not been possible to dispense with experimental animals completely and further research is needed to provide a road map to robust alternatives before their use can be fully discontinued.

How to cite this article: Kiani AK, Pheby D, Henehan G, Brown R, Sieving P, Sykora P, Marks R, Falsini B, Capodicasa N, Miertus S, Lorusso L, Dondossola D, Tartaglia GM, Ergoren MC, Dundar M, Michelini S, Malacarne D, Bonetti G, Dautaj A, Donato K, Medori MC, Beccari T, Samaja M, Connelly ST, Martin D, Morresi A, Bacu A, Herbst KL, Kapustin M, Stuppia L, Lumer L, Farronato G, Bertelli M. Ethical considerations regarding animal experimentation. J Prev Med Hyg 2022;63(suppl.3):E255-E266. https://doi.org/10.15167/2421-4248/jpmh2022.63.2S3.2768

Introduction

Animal model-based research has been performed for a very long time. Ever since the 5 th century B.C., reports of experiments involving animals have been documented, but an increase in the frequency of their utilization has been observed since the 19 th century [ 1 ]. Most institutions for medical research around the world use non-human animals as experimental subjects [ 2 ]. Such animals might be used for research experimentations to gain a better understanding of human diseases or for exploring potential treatment options [ 2 ]. Even those animals that are evolutionarily quite distant from humans, such as Drosophila melanogaster , Zebrafish ( Danio rerio ) and Caenorhabditis elegans , share physiological and genetic similarities with human beings [ 2 ]; therefore animal experimentation can be of great help for the advancement of medical science [ 2 ].

For animal experimentation, the major assumption is that the animal research will be of benefit to humans. There are many reasons that highlight the significance of animal use in biomedical research. One of the major reasons is that animals and humans share the same biological processes. In addition, vertebrates have many anatomical similarities (all vertebrates have lungs, a heart, kidneys, liver and other organs) [ 3 ]. Therefore, these similarities make certain animals more suitable for experiments and for providing basic training to young researchers and students in different fields of biological and biomedical sciences [ 3 ]. Certain animals are susceptible to various health problems that are similar to human diseases such as diabetes, cancer and heart disease [ 4 ]. Furthermore, there are genetically modified animals that are used to obtain pathological phenotypes [ 5 ]. A significant benefit of animal experimentation is that test species can be chosen that have a much shorter life cycle than humans. Therefore, animal models can be studied throughout their life span and for several successive generations, an essential element for the understanding of disease progression along with its interaction with the whole organism throughout its lifetime [ 6 ].

Animal models often play a critical role in helping researchers who are exploring the efficacy and safety of potential medical treatments and drugs. They help to identify any dangerous or undesired side effects, such as birth defects, infertility, toxicity, liver damage or any potential carcinogenic effects [ 7 ]. Currently, U.S. Federal law, for example, requires that non-human animal research is used to demonstrate the efficacy and safety of any new treatment options before proceeding to trials on humans [ 8 ]. Of course, it is not only humans benefit from this research and testing, since many of the drugs and treatments that are developed for humans are routinely used in veterinary clinics, which help animals live longer and healthier lives [ 4 ].

COVID-19 AND THE NEED FOR ANIMAL MODELS

When COVID-19 struck, there was a desperate need for research on the disease, its effects on the brain and body and on the development of new treatments for patients with the disease. Early in the disease it was noticed that those with the disease suffered a loss of smell and taste, as well as neurological and psychiatric symptoms, some of which lasted long after the patients had “survived” the disease [ 9-15 ]. As soon as the pandemic started, there was a search for appropriate animal models in which to study this unknown disease [ 16 , 17 ]. While genetically modified mice and rats are the basic animal models for neurological and immunological research [ 18 , 19 ] the need to understand COVID-19 led to a range of animal models; from fruit flies [ 20 ] and Zebrafish [ 21 ] to large mammals [ 22 , 23 ] and primates [ 24 , 25 ]. And it was just not one animal model that was needed, but many, because different aspects of the disease are best studied in different animal models [ 16 , 25 , 26 ]. There is also a need to study the transmission pathways of the zoonosis: where does it come from, what are the animal hosts and how is it transferred to humans [ 27 ]?

There has been a need for animal models for understanding the pathophysiology of COVID-19 [ 28 ], for studying the mechanisms of transmission of the disease [ 16 ], for studying its neurobiology [ 29 , 30 ] and for developing new vaccines [ 31 ]. The sudden onset of the COVID-19 pandemic has highlighted the fact that animal research is necessary, and that the curtailment of such research has serious consequences for the health of both humans and animals, both wild and domestic [ 32 ] As highlighted by Adhikary et al. [ 22 ] and Genzel et al. [ 33 ] the coronavirus has made clear the necessity for animal research and the danger in surviving future such pandemics if animal research is not fully supported. Genzel et al. [ 33 ], in particular, take issue with the proposal for a European ban on animal testing. Finally, there is a danger in bypassing animal research in developing new vaccines for diseases such as COVID-19 [ 34 ]. The purpose of this paper is to show that, while animal research is necessary for the health of both humans and animals, there is a need to carry out such experimentation in a controlled and humane manner. The use of alternatives to animal research such as cultured human cells and computer modeling may be a useful adjunct to animal studies but will require that such methods are more readily accessible to researchers and are not a replacement for animal experimentation.

Pros and cons of animal experimentation

Arguments against animal experimentation.

A fundamental question surrounding this debate is to ask whether it is appropriate to use animals for medical research. Is our acceptance that animals have a morally lower value or standard of life just a case of speciesism [ 35 ]? Nowadays, most people agree that animals have a moral status and that needlessly hurting or abusing pets or other animals is unacceptable. This represents something of a change from the historical point of view where animals did not have any moral status and the treatment of animals was mostly subservient to maintaining the health and dignity of humans [ 36 ].

Animal rights advocates strongly argue that the moral status of non-human animals is similar to that of humans, and that animals are entitled to equality of treatment. In this view, animals should be treated with the same level of respect as humans, and no one should have the right to force them into any service or to kill them or use them for their own goals. One aspect of this argument claims that moral status depends upon the capacity to suffer or enjoy life [ 37 ].

In terms of suffering and the capacity of enjoying life, many animals are not very different from human beings, as they can feel pain and experience pleasure [ 38 ]. Hence, they should be given the same moral status as humans and deserve equivalent treatment. Supporters of this argument point out that according animals a lower moral status than humans is a type of prejudice known as “speciesism” [ 38 ]. Among humans, it is widely accepted that being a part of a specific race or of a specific gender does not provide the right to ascribe a lower moral status to the outsiders. Many advocates of animal rights deploy the same argument, that being human does not give us sufficient grounds declare animals as being morally less significant [ 36 ].

ARGUMENTS IN FAVOR OF ANIMAL EXPERIMENTATION

Those who support animal experimentation have frequently made the argument that animals cannot be elevated to be seen as morally equal to humans [ 39 ]. Their main argument is that the use of the terms “moral status” or “morality” is debatable. They emphasize that we must not make the error of defining a quality or capacity associated with an animal by using the same adjectives used for humans [ 39 ]. Since, for the most part, animals do not possess humans’ cognitive capabilities and lack full autonomy (animals do not appear to rationally pursue specific goals in life), it is argued that therefore, they cannot be included in the moral community [ 39 ]. It follows from this line of argument that, if animals do not possess the same rights as human beings, their use in research experimentation can be considered appropriate [ 40 ]. The European and the American legislation support this kind of approach as much as their welfare is respected.

Another aspect of this argument is that the benefits to human beings of animal experimentation compensate for the harm caused to animals by these experiments.

In other words, animal harm is morally insignificant compared to the potential benefits to humans. Essentially, supporters of animal experimentation claim that human beings have a higher moral status than animals and that animals lack certain fundamental rights accorded to humans. The potential violations of animal rights during animal research are, in this way, justified by the greater benefits to mankind [ 40 , 41 ]. A way to evaluate when the experiments are morally justified was published in 1986 by Bateson, which developed the Bateson’s Cube [ 42 ]. The Cube has three axes: suffering, certainty of benefit and quality of research. If the research is high-quality, beneficial, and not inflicting suffering, it will be acceptable. At the contrary, painful, low-quality research with lower likelihood of success will not be acceptable [ 42 , 43 ].

Impact of experimentations on animals

Ability to feel pain and distress.

Like humans, animal have certain physical as well as psychological characteristics that make their use for experimentation controversial [ 44 ].

In the last few decades, many studies have increased knowledge of animal awareness and sentience: they indicate that animals have greater potential to experience damage than previously appreciated and that current rights and protections need to be reconsidered [ 45 ]. In recent times, scientists as well as ethicists have broadly acknowledged that animals can also experience distress and pain [ 46 ]. Potential sources of such harm arising from their use in research include disease, basic physiological needs deprivation and invasive procedures [ 46 ]. Moreover, social deprivation and lack of the ability to carry out their natural behaviors are other causes of animal harm [ 46 ]. Several studies have shown that, even in response to very gentle handling and management, animals can show marked alterations in their physiological and hormonal stress markers [ 47 ].

In spite of the fact that suffering and pain are personalized experiences, several multi-disciplinary studies have provided clear evidence of animals experiencing pain and distress. In particular, some animal species have the ability to express pain similarly to human due to common psychological, neuroanatomical and genetic characteristics [ 48 ]. Similarly, animals share a resemblance to humans in their developmental, genetic and environmental risk factors for psychopathology. For instance, in many species, it has been shown that fear operates within a less organized subcortical neural circuit than pain [ 49 , 50 ]. Various types of depression and anxiety disorders like posttraumatic stress disorder have also been reported in mammals [ 51 ].

PSYCHOLOGICAL CAPABILITIES OF ANIMALS

Some researchers have suggested that besides their ability to experience physical and psychological pain and distress, some animals also exhibit empathy, self-awareness and language-like capabilities. They also demonstrate tools-linked cognizance, pleasure-seeking and advanced problem-solving skills [ 52 ]. Moreover, mammals and birds exhibit playful behavior, an indicator of the capacity to experience pleasure. Other taxa such as reptiles, cephalopods and fishes have also been observed to display playful behavior, therefore the current legislation prescribes the use of environmental enrichers [ 53 ]. The presence of self-awareness ability, as assessed by mirror self-recognition, has been reported in magpies, chimpanzees and other apes, and certain cetaceans [ 54 ]. Recently, another study has revealed that crows have the ability to create and use tools that involve episodic-like memory formation and its retrieval. From these findings, it may be suggested that crows as well as related species show evidence of flexible learning strategies, causal reasoning, prospection and imagination that are similar to behavior observed in great apes [ 55 ]. In the context of resolving the ethical dilemmas about animal experimentation, these observations serve to highlight the challenges involved [ 56 , 57 ].

Ethics, principles and legislation in animal experimentation

Ethics in animal experimentation.

Legislation around animal research is based on the idea of the moral acceptability of the proposed experiments under specific conditions [ 58 ]. The significance of research ethics that ensures proper treatment of experimental animals [ 58 ]. To avoid undue suffering of animals, it is important to follow ethical considerations during animal studies [ 1 ]. It is important to provide best human care to these animals from the ethical and scientific point of view [ 1 ]. Poor animal care can lead to experimental outcomes [ 1 ]. Thus, if experimental animals mistreated, the scientific knowledge and conclusions obtained from experiments may be compromised and may be difficult to replicate, a hallmark of scientific research [ 1 ]. At present, most ethical guidelines work on the assumption that animal experimentation is justified because of the significant potential benefits to human beings. These guidelines are often permissive of animal experimentation regardless of the damage to the animal as long as human benefits are achieved [ 59 ].

PRINCIPLE OF THE 4 RS

Although animal experimentation has resulted in many discoveries and helped in the understanding numerous aspects of biological science, its use in various sectors is strictly controlled. In practice, the proposed set of animal experiments is usually considered by a multidisciplinary Ethics Committee before work can commence [ 60 ]. This committee will review the research protocol and make a judgment as to its sustainability. National and international laws govern the utilization of animal experimentation during research and these laws are mostly based on the universal doctrine presented by Russell and Burch (1959) known as principle of the 3 Rs. The 3Rs referred to are Reduction, Refinement and Replacement, and are applied to protocols surrounding the use of animals in research. Some researchers have proposed another “R”, of responsibility for the experimental animal as well as for the social and scientific status of the animal experiments [ 61 ]. Thus, animal ethics committees commonly review research projects with reference to the 4 Rs principles [ 62 ].

The first “R”, Reduction means that the experimental design is examined to ensure that researchers have reduced the number of experimental animals in a research project to the minimum required for reliable data [ 59 ]. Methods used for this purpose include improved experimental design, extensive literature search to avoid duplication of experiments [ 35 ], use of advanced imaging techniques, sharing resources and data, and appropriate statistical data analysis that reduce the number of animals needed for statistically significant results [ 2 , 63 ].

The second “R”, Refinement involves improvements in procedure that minimize the harmful effects of the proposed experiments on the animals involved, such as reducing pain, distress and suffering in a manner that leads to a general improvement in animal welfare. This might include for example improved living conditions for research animals, proper training of people handling animals, application of anesthesia and analgesia when required and the need for euthanasia of the animals at the end of the experiment to curtail their suffering [ 63 ].

The third “R”, Replacement refers to approaches that replace or avoid the use of experimental animals altogether. These approaches involve use of in silico methods/computerized techniques/software and in vitro methods like cell and tissue culture testing, as well as relative replacement methods by use of invertebrates like nematode worms, fruit flies and microorganisms in place of vertebrates and higher animals [ 1 ]. Examples of proper application of these first “3R2 principles are the use of alternative sources of blood, the exploitation of commercially used animals for scientific research, a proper training without use of animals and the use of specimen from previous experiments for further researches [ 64-67 ].

The fourth “R”, Responsibility refers to concerns around promoting animal welfare by improvements in experimental animals’ social life, development of advanced scientific methods for objectively determining sentience, consciousness, experience of pain and intelligence in the animal kingdom, as well as effective involvement in the professionalization of the public discussion on animal ethics [ 68 ].

OTHER ASPECTS OF ANIMAL RESEARCH ETHICS

Other research ethics considerations include having a clear rationale and reasoning for the use of animals in a research project. Researchers must have reasonable expectation of generating useful data from the proposed experiment. Moreover, the research study should be designed in such a way that it should involve the lowest possible sample size of experimental animals while producing statistically significant results [ 35 ].

All individual researchers that handle experimental animals should be properly trained for handling the particular species involved in the research study. The animal’s pain, suffering and discomfort should be minimized [ 69 ]. Animals should be given proper anesthesia when required and surgical procedures should not be repeated on same animal whenever possible [ 69 ]. The procedure of humane handling and care of experimental animals should be explicitly detailed in the research study protocol. Moreover, whenever required, aseptic techniques should be properly followed [ 70 ]. During the research, anesthetization and surgical procedures on experimental animals should only be performed by professionally skilled individuals [ 69 ].

The Animal Research: Reporting of In Vivo Experiments (ARRIVE) guidelines that are issued by the National Center for the Replacement, Refinement, and Reduction of Animals in Research (NC3Rs) are designed to improve the documentation surrounding research involving experimental animals [ 70 ]. The checklist provided includes the information required in the various sections of the manuscript i.e. study design, ethical statements, experimental procedures, experimental animals and their housing and husbandry, and more [ 70 ].

It is critical to follow the highest ethical standards while performing animal experiments. Indeed, most of the journals refuse to publish any research data that lack proper ethical considerations [ 35 ].

INVESTIGATORS’ ETHICS

Since animals have sensitivity level similar to the human beings in terms of pain, anguish, survival instinct and memory, it is the responsibility of the investigator to closely monitor the animals that are used and identify any sign of distress [ 71 ]. No justification can rationalize the absence of anesthesia or analgesia in animals that undergo invasive surgery during the research [ 72 ]. Investigators are also responsible for giving high-quality care to the experimental animals, including the supply of a nutritious diet, easy water access, prevention of and relief from any pain, disease and injury, and appropriate housing facilities for the animal species [ 73 ]. A research experiment is not permitted if the damage caused to the animal exceeds the value of knowledge gained by that experiment. No scientific advancement based on the destruction and sufferings of another living being could be justified. Besides ensuring the welfare of animals involved, investigators must also follow the applicable legislation [ 74 , 75 ].

To promote the comfort of experimental animals in England, an animal protection society named: ‘The Society for the Preservation of Cruelty to Animals’ (now the Royal Society for the Prevention of Cruelty to Animals) was established (1824) that aims to prevent cruelty to animal [ 76 ].

ANIMAL WELFARE LAWS

Legislation for animal protection during research has long been established. In 1876 the British Parliament sanctioned the ‘Cruelty to Animals Act’ for animal protection. Russell and Burch (1959) presented the ‘3 Rs’ principles: Replacement, Reduction and Refinement, for use of animals during research [ 61 ]. Almost seven years later, the U.S.A also adopted regulations for the protection of experimental animals by enacting the Laboratory Animal Welfare Act of 1966 [ 60 ]. In Brazil, the Arouca Law (Law No. 11,794/08) regulates the animal use in scientific research experiments [ 76 ].

These laws define the breeding conditions, and regulate the use of animals for scientific research and teaching purposes. Such legal provisions control the use of anesthesia, analgesia or sedation in experiments that could cause distress or pain to experimental animals [ 59 , 76 ]. These laws also stress the need for euthanasia when an experiment is finished, or even during the experiment if there is any intense suffering for the experimental animal [ 76 ].

Several national and international organizations have been established to develop alternative techniques so that animal experimentation can be avoided, such as the UK-based National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) ( www.caat.jhsph.edu ), the European Centre for the Validation of Alternative Methods (ECVAM) [ 77 ], the Universities Federation for Animal Welfare (UFAW) ( www.ufaw.org.uk ), The Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM) [ 78 ], and The Center for Alternatives to Animal Testing (CAAT) ( www.caat.jhsph.edu ). The Brazilian ‘Arouca Law’ also constitutes a milestone, as it has created the ‘National Council for the Control of Animal Experimentation’ (CONCEA) that deals with the legal and ethical issues related to the use of experimental animals during scientific research [ 76 ].

Although national as well as international laws and guidelines have provided basic protections for experimental animals, the current regulations have some significant discrepancies. In the U.S., the Animal Welfare Act excludes rats, mice and purpose-bred birds, even though these species comprise almost 90% of the animals that are used for research purpose [ 79 ]. On the other hand, certain cats and dogs are getting special attention along with extra protection. While the U.S. Animal Welfare Act ignores birds, mice and rats, the U.S. guidelines that control research performed using federal funding ensure protections for all vertebrates [ 79 , 80 ].

Living conditions of animals

Choice of the animal model.

Based on all the above laws and regulations and in line with the deliberations of ethical committees, every researcher must follow certain rules when dealing with animal models.

Before starting any experimental work, thorough research should be carried out during the study design phase so that the unnecessary use of experimental animals is avoided. Nevertheless, certain research studies may have compelling reasons for the use of animal models, such as the investigation of human diseases and toxicity tests. Moreover, animals are also widely used in the training of health professionals as well as in training doctors in surgical skills [ 1 , 81 ].

Researcher should be well aware of the specific traits of the animal species they intend to use in the experiment, such as its developmental stages, physiology, nutritional needs, reproductive characteristics and specific behaviors. Animal models should be selected on the basis of the study design and the biological relevance of the animal [ 1 ].

Typically, in early research, non-mammalian models are used to get rapid insights into research problems such as the identification of gene function or the recognition of novel therapeutic options. Thus, in biomedical and biological research, among the most commonly used model organisms are the Zebrafish, the fruit fly Drosophila melanogaster and the nematode Caenorhabditis elegans . The main advantage of these non-mammalian animal models is their prolific reproducibility along with their much shorter generation time. They can be easily grown in any laboratory setting, are less expensive than the murine animal models and are somewhat more powerful than the tissue and cell culture approaches [ 82 ].

Caenorhabditis elegans is a small-sized nematode with a short life cycle and that exists in large populations and is relatively inexpensive to cultivate. Scientists have gathered extensive knowledge of the genomics and genetics of Caenorhabditis elegans ; but Caenorhabditis elegans models, while very useful in some respects, are unable to represent all signaling pathways found in humans. Furthermore, due to its short life cycle, scientists are unable to investigate long term effects of test compounds or to analyze primary versus secondary effects [ 6 ].

Similarly, the fruit fly Drosophila melanogaster has played a key role in numerous biomedical discoveries. It is small in size, has a short life cycle and large population size, is relatively inexpensive to breed, and extensive genomics and genetics information is available [ 6 ]. However, its respiratory, cardiovascular and nervous systems differ considerably from human beings. In addition, its immune system is less developed when compared to vertebrates, which is why effectiveness of a drug in Drosophila melanogaster may not be easily extrapolated to humans [ 83 ].

The Zebrafish ( Danio rerio ) is a small freshwater teleost, with transparent embryos, providing easy access for the observation of organogenesis and its manipulation. Therefore, Zebrafish embryos are considered good animal models for different human diseases like tuberculosis and fetal alcohol syndrome and are useful as neurodevelopmental research models. However, Zebrafish has very few mutant strains available, and its genome has numerous duplicate genes making it impossible to create knockout strains, since disrupting one copy of the gene will not disrupt the second copy of that gene. This feature limits the use of Zebrafish as animal models to study human diseases. Additionally they are rather expensive, have long life cycle, and genomics and genetics studies are still in progress [ 82 , 84 ].

Thus, experimentation on these three animals might not be equivalent to experimentation on mammals. Mammalian animal model are most similar to human beings, so targeted gene replacement is possible. Traditionally, mammals like monkey and mice have been the preferred animal models for biomedical research because of their evolutionary closeness to humans. Rodents, particularly mice and rats, are the most frequently used animal models for scientific research. Rats are the most suitable animal model for the study of obesity, shock, peritonitis, sepsis, cancer, intestinal operations, spleen, gastric ulcers, mononuclear phagocytic system, organ transplantations and wound healing. Mice are more suitable for studying burns, megacolon, shock, cancer, obesity, and sepsis as mentioned previously [ 85 ].

Similarly, pigs are mostly used for stomach, liver and transplantation studies, while rabbits are suitable for the study of immunology, inflammation, vascular biology, shock, colitis and transplantations. Thus, the choice of experimental animal mainly depends upon the field of scientific research under consideration [ 1 ].

HOUSING AND ENVIRONMENTAL ENRICHMENT

Researchers should be aware of the environment and conditions in which laboratory animals are kept during research, and they also need to be familiar with the metabolism of the animals kept in vivarium, since their metabolism can easily be altered by different factors such as pain, stress, confinement, lack of sunlight, etc. Housing conditions alter animal behavior, and this can in turn affect experimental results. By contrast, handling procedures that feature environmental enrichment and enhancement help to decrease stress and positively affect the welfare of the animals and the reliability of research data [ 74 , 75 ].

In animals, distress- and agony-causing factors should be controlled or eliminated to overcome any interference with data collection as well as with interpretation of the results, since impaired animal welfare leads to more animal usage during experiment, decreased reliability and increased discrepancies in results along with the unnecessary consumption of animal lives [ 86 ].

To reduce the variation or discrepancies in experimental data caused by various environmental factors, experimental animals must be kept in an appropriate and safe place. In addition, it is necessary to keep all variables like humidity, airflow and temperature at levels suitable for those species, as any abrupt variation in these factors could cause stress, reduced resistance and increased susceptibility to infections [ 74 ].

The space allotted to experimental animals should permit them free movement, proper sleep and where feasible allow for interaction with other animals of the same species. Mice and rats are quite sociable animals and must, therefore, be housed in groups for the expression of their normal behavior. Usually, laboratory cages are not appropriate for the behavioral needs of the animals. Therefore, environmental enrichment is an important feature for the expression of their natural behavior that will subsequently affect their defense mechanisms and physiology [ 87 ].

The features of environmental enrichment must satisfy the animals’ sense of curiosity, offer them fun activities, and also permit them to fulfill their behavioral and physiological needs. These needs include exploring, hiding, building nests and gnawing. For this purpose, different things can be used in their environment, such as PVC tubes, cardboard, igloos, paper towel, cotton, disposable masks and paper strips [ 87 ].

The environment used for housing of animals must be continuously controlled by appropriate disinfection, hygiene protocols, sterilization and sanitation processes. These steps lead to a reduction in the occurrence of various infectious agents that often found in vivarium, such as Sendai virus, cestoda and Mycoplasma pulmonis [ 88 ].

Euthanasia is a term derived from Greek, and it means a death without any suffering. According to the Brazilian Arouca Law (Article 14, Chapter IV, Paragraphs 1 and 2), an animal should undergo euthanasia, in strict compliance with the requirements of each species, when the experiment ends or during any phase of the experiment, wherever this procedure is recommended and/or whenever serious suffering occurs. If the animal does not undergo euthanasia after the intervention it may leave the vivarium and be assigned to suitable people or to the animal protection bodies, duly legalized [ 1 ].

Euthanasia procedures must result in instant loss of consciousness which leads to respiratory or cardiac arrest as well as to complete brain function impairment. Another important aspect of this procedure is calm handling of the animal while taking it out of its enclosure, to reduce its distress, suffering, anxiety and fear. In every research project, the study design should include the details of the appropriate endpoints of these experimental animals, and also the methods that will be adopted. It is important to determine the appropriate method of euthanasia for the animal being used. Another important point is that, after completing the euthanasia procedure, the animal’s death should be absolutely confirmed before discarding their bodies [ 87 , 89 ].

Relevance of animal experimentations and possible alternatives

Relevance of animal experiments and their adverse effects on human health.

One important concern is whether human diseases, when inflicted on experimental animals, adequately mimic the progressions of the disease and the treatment responses observed in humans. Several research articles have made comparisons between human and animal data, and indicated that the results of animals’ research could not always be reliably replicated in clinical research among humans. The latest systematic reviews about the treatment of different clinical conditions including neurology, vascular diseases and others, have established that the results of animal studies cannot properly predict human outcomes [ 59 , 90 ].

At present, the reliability of animal experiments for extrapolation to human health is questionable. Harmful effects may occur in humans because of misleading results from research conducted on animals. For instance, during the late fifties, a sedative drug, thalidomide, was prescribed for pregnant women, but some of the women using that drug gave birth to babies lacking limbs or with foreshortened limbs, a condition called phocomelia. When thalidomide had been tested on almost all animal models such as rats, mice, rabbits, dogs, cats, hamsters, armadillos, ferrets, swine, guinea pig, etc., this teratogenic effect was observed only occasionally [ 91 ]. Similarly, in 2006, the compound TGN 1412 was designed as an immunomodulatory drug, but when it was injected into six human volunteer, serious adverse reactions were observed resulting from a deadly cytokine storm that in turn led to disastrous systemic organ failure. TGN 1412 had been tested successfully in rats, mice, rabbits, and non-human primates [ 92 ]. Moreover, Bailey (2008) reported 90 HIV vaccines that had successful trial results in animals but which failed in human beings [ 93 ]. Moreover, in Parkinson disease, many therapeutic options that have shown promising results in rats and non-human primate models have proved harmful in humans. Hence, to analyze the relevance of animal research to human health, the efficacy of animal experimentation should be examined systematically [ 94 , 95 ]. At the same time, the development of hyperoxaluria and renal failure (up to dialysis) after ileal-jejunal bypass was unexpected because this procedure was not preliminarily evaluated on an animal model [ 96 ].

Several factors play a role in the extrapolation of animal-derived data to humans, such as environmental conditions and physiological parameters related to stress, age of the experimental animals, etc. These factors could switch on or off genes in the animal models that are specific to species and/or strains. All these observations challenge the reliability and suitability of animal experimentation as well as its objectives with respect to human health [ 76 , 92 ].

ALTERNATIVE TO ANIMAL EXPERIMENTATION/DEVELOPMENT OF NEW PRODUCTS AND TECHNIQUES TO AVOID ANIMAL SACRIFICE IN RESEARCH

Certainly, in vivo animal experimentation has significantly contributed to the development of biological and biomedical research. However it has the limitations of strict ethical issues and high production cost. Some scientists consider animal testing an ineffective and immoral practice and therefore prefer alternative techniques to be used instead of animal experimentation. These alternative methods involve in vitro experiments and ex vivo models like cell and tissue cultures, use of plants and vegetables, non-invasive human clinical studies, use of corpses for studies, use of microorganisms or other simpler organism like shrimps and water flea larvae, physicochemical techniques, educational software, computer simulations, mathematical models and nanotechnology [ 97 ]. These methods and techniques are cost-effective and could efficiently replace animal models. They could therefore, contribute to animal welfare and to the development of new therapies that can identify the therapeutics and related complications at an early stage [ 1 ].

The National Research Council (UK) suggested a shift from the animal models toward computational models, as well as high-content and high-throughput in vitro methods. Their reports highlighted that these alternative methods could produce predictive data more affordably, accurately and quickly than the traditional in vivo or experimental animal methods [ 98 ].

Increasingly, scientists and the review boards have to assess whether addressing a research question using the applied techniques of advanced genetics, molecular, computational and cell biology, and biochemistry could be used to replace animal experiments [ 59 ]. It must be remembered that each alternative method must be first validated and then registered in dedicated databases.

An additional relevant concern is how precisely animal data can mirror relevant epigenetic changes and human genetic variability. Langley and his colleagues have highlighted some of the examples of existing and some emerging non-animal based research methods in the advanced fields of neurology, orthodontics, infectious diseases, immunology, endocrine, pulmonology, obstetrics, metabolism and cardiology [ 99 ].

IN SILICO SIMULATIONS AND INFORMATICS

Several computer models have been built to study cardiovascular risk and atherosclerotic plaque build-up, to model human metabolism, to evaluate drug toxicity and to address other questions that were previously approached by testing in animals [ 100 ].

Computer simulations can potentially decrease the number of experiments required for a research project, however simulations cannot completely replace laboratory experiments. Unfortunately, not all the principles regulating biological systems are known, and computer simulation provide only an estimation of possible effects due to the limitations of computer models in comparison with complex human tissues. However, simulation and bio-informatics are now considered essential in all fields of science for their efficiency in using the existing knowledge for further experimental designs [ 76 ].

At present, biological macromolecules are regularly simulated at various levels of detail, to predict their response and behavior under certain physical conditions, chemical exposures and stimulations. Computational and bioinformatic simulations have significantly reduced the number of animals sacrificed during drug discovery by short listing potential candidate molecules for a drug. Likewise, computer simulations have decreased the number of animal experiments required in other areas of biological science by efficiently using the existing knowledge. Moreover, the development of high definition 3D computer models for anatomy with enhanced level of detail, it may make it possible to reduce or eliminate the need for animal dissection during teaching [ 101 , 102 ].

3D CELL-CULTURE MODELS AND ORGANS-ON-CHIPS

In the current scenario of rapid advancement in the life sciences, certain tissue models can be built using 3D cell culture technology. Indeed, there are some organs on micro-scale chip models used for mimicking the human body environment. 3D models of multiple organ systems such as heart, liver, skin, muscle, testis, brain, gut, bone marrow, lungs and kidney, in addition to individual organs, have been created in microfluidic channels, re-creating the physiological chemical and physical microenvironments of the body [ 103 ]. These emerging techniques, such as the biomedical/biological microelectromechanical system (Bio-MEMS) or lab-on-a-chip (LOC) and micro total analysis systems (lTAS) will, in the future, be a useful substitute for animal experimentation in commercial laboratories in the biotechnology, environmental safety, chemistry and pharmaceutical industries. For 3D cell culture modeling, cells are grown in 3D spheroids or aggregates with the help of a scaffold or matrix, or sometimes using a scaffold-free method. The 3D cell culture modeling conditions can be altered to add proteins and other factors that are found in a tumor microenvironment, for example, or in particular tissues. These matrices contain extracellular matrix components such as proteins, glycoconjugates and glycosaminoglycans that allow for cell communication, cell to cell contact and the activation of signaling pathways in such a way that the morphological and functional differentiation of these cells can accurately mimic their environment in vivo . This methodology, in time, will bridge the gap between in vivo and in vitro drug screening, decreasing the utilization of animal models during research [ 104 ].

ALTERNATIVES TO MICROBIAL CULTURE MEDIA AND SERUM-FREE ANIMAL CELL CULTURES

There are moves to reduce the use of animal derived products in many areas of biotechnology. Microbial culture media peptones are mostly made by the proteolysis of farmed animal meat. However, nowadays, various suppliers provide peptones extracted from yeast and plants. Although the costs of these plant-extracted peptones are the same as those of animal peptones, plant peptones are more environmentally favorable since less plant material and water are required for them to grow, compared with the food grain and fodder needed for cattle that are slaughtered for animal peptone production [ 105 ].

Human cell culture is often carried out in a medium that contains fetal calf serum, the production of which involves animal (cow) sacrifice or suffering. In fact, living pregnant cows are used and their fetuses removed to harvest the serum from the fetal blood. Fetal calf serum is used because it is a natural medium rich in all the required nutrients and significantly increases the chances of successful cell growth in culture. Scientists are striving to identify the factors and nutrients required for the growth of various types of cells, with a view to eliminating the use of calf serum. At present, most cell lines could be cultured in a chemically-synthesized medium without using animal products. Furthermore, data from chemically-synthesized media experiments may have better reproducibility than those using animal serum media, since the composition of animal serum does change from batch to batch on the basis of animals’ gender, age, health and genetic background [ 76 ].

ALTERNATIVES TO ANIMAL-DERIVED ANTIBODIES

Animal friendly affinity reagents may act as an alternative to antibodies produced, thereby removing the need for animal immunization. Typically, these antibodies are obtained in vitro by yeast, phage or ribosome display. In a recent review, a comparative analysis between animal friendly affinity reagents and animal derived-antibodies showed that the affinity reagents have superior quality, are relatively less time consuming, have more reproducibility and are more reliable and are cost-effective [ 106 , 107 ].

Conclusions

Animal experimentation led to great advancement in biological and biomedical sciences and contributed to the discovery of many drugs and treatment options. However, such experimentation may cause harm, pain and distress to the animals involved. Therefore, to perform animal experimentations, certain ethical rules and laws must be strictly followed and there should be proper justification for using animals in research projects. Furthermore, during animal experimentation the 4 Rs principles of reduction, refinement, replacement and responsibility must be followed by the researchers. Moreover, before beginning a research project, experiments should be thoroughly planned and well-designed, and should avoid unnecessary use of animals. The reliability and reproducibility of animal experiments should also be considered. Whenever possible, alternative methods to animal experimentation should be adopted, such as in vitro experimentation, cadaveric studies, and computer simulations.

While much progress has been made on reducing animal experimentation there is a need for greater awareness of alternatives to animal experiments among scientists and easier access to advanced modeling technologies. Greater research is needed to define a roadmap that will lead to the elimination of all unnecessary animal experimentation and provide a framework for adoption of reliable alternative methodologies in biomedical research.

Acknowledgements

This research was funded by the Provincia Autonoma di Bolzano in the framework of LP 15/2020 (dgp 3174/2021).

Conflicts of interest statement

Authors declare no conflict of interest.

Author's contributions

MB: study conception, editing and critical revision of the manuscript; AKK, DP, GH, RB, Paul S, Peter S, RM, BF, NC, SM, LL, DD, GMT, MCE, MD, SM, Daniele M, GB, AD, KD, MCM, TB, MS, STC, Donald M, AM, AB, KLH, MK, LS, LL, GF: literature search, editing and critical revision of the manuscript. All authors have read and approved the final manuscript.

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Fantastic Fun & Learning

Fun learning activities and things to do with kids

Fun Forest Science Activities for Kids

By Shaunna Evans 7 Comments · This content may contain affiliate links.

These fun forest science activities are a wonderful way to help kids learn about the forest and parts of a tree.  They are great tree theme science investigations for fall or any time of year. You can add these tree activities to your nature studies, fall activities, Earth Day activities, Arbor Day activities or tree theme units.

FIND EVEN MORE FOREST THEME ACTIVITIES IN OUR PRINTABLE PRESCHOOL LESSON PLANS .

Teach kids about the parts of a tree with this tree snack science activity .

Investigate autumn leaf chromatography with this experiment from Life with Moore Babies.

Explore the sense of touch with this nature sensory board from Fun at Home with Kids.

Learn about the parts of a tree and more with this tree track from Play Trains!

Find symmetry in nature and create your own symmetrical designs with natural materials using these ideas from Buggy and Buddy.

Learn about the weather with this pine cone predictor experiment from the In the Woods theme post by Sorting Sprinkles.

Your kids will love Growing their Own Mushrooms with this fun nature science experiment.

This Sorting Leaves & Seeds activity is the perfect fall science activity for kids.

This How Old is a Tree science experiment is a great way to get your kids learning while playing outside.

This Pine Branch Hardiness STEM Experiment is a great fall science experiment for your kids to do.

Your kids will love watching leaves change color in this Fall Leaf Science Experiment .

Your kids will love Exploring How Water Travels Through Leaves in this science experiment.

MORE FOREST FUN WITH PRINTABLE LESSON PLANS

Save time and get right to the playful learning with our printable lesson plan sets. Each set includes over 30 playful learning activities related to the theme, and we’ve provided different versions for home preschool families and classroom teachers so all activities are geared directly toward your needs.

This set currently includes active hands-on learning ideas and the following printables:

1) Forest vs Ocean Animals Sorting Activity 2) Forest Animal Addition Activity 3) The Forest Emergent Reader (3 Versions) 4) Forest Roll and Graph Cube and Recording Sheet 5) “A Walk in the Forest” Math Game Board 6) Number Cubes for “A Walk in The Forest” and other Math Games (4 Versions) 7) Animal Tracks Matching Cards (in color and b/w) 8) Forest Animal Picture Cards (in color and b/w) 9) Forest Animal Pictures with Labels (in color and b/w) 10) Forest Animal Word Cards (in color and b/w) 11) Forest Animal Syllable Sorting Activity Mats 12) Forest Letter Puzzles (in color and b/w) 13) Teddy Bear Pattern Prompt Cards

GET YOUR LESSON PLANS

Home Preschool Forest Theme Lesson Plans

Preschool Classroom Forest Theme Lesson Plans

Also available on  Teachers Pay Teachers .

More Forest Posts from the Discover and Explore Co-hosts

Tips for Hiking with Kids (KC Edventures)

Finding Symmetry in Nature (Buggy and Buddy)

Fall Tree Snack (Fantastic Fun and Learning)

Arranging a Forest (Teach Preschool)

Invitation to Play: Create a Forest (Twodaloo)

Reader Interactions

January 11, 2017 at 9:29 pm

This post is amazing! I love it. specially the “Trees track train” It’s very environmental.

Thanks for sharing.

January 11, 2017 at 9:21 pm

This is very interesting! kids will love this fun activity.

Thanks for sharing this idea.

January 30, 2020 at 9:42 pm

That’s wonderful to hear! Thanks for sharing, Lorenzo.

August 22, 2014 at 11:09 am

Just saw this on Pinterest. (Repinned) Great post. I am showing it to my son to see what he wants to try. Thanks for the good ideas. I am in love with the train track tree!

Sarah @ How Wee Learn

September 15, 2013 at 1:51 pm

Such great ideas – I love the train tracks tree too! Such a creative Mama!

September 13, 2013 at 10:12 am

That tree track looks awesome! Going to check it out now!

September 13, 2013 at 8:44 pm

Isn’t that so cool? I just love it! Play Trains is an awesome site.

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50 Easy Science Activities For Preschoolers

Curious kids turn into junior scientists with these fun and easy preschool science experiments . Th ese science activities for preschoolers are doable and use simple supplies for home or in the classroom. Plus, you can scale any of these activities to use with kindergarten through elementary-age students!

Science for Preschoolers Engages the Senses

Preschool science encourages observations of the five senses, including sight, sound, touch, smell, and sometimes taste. The more kids can fully immerse themselves in an activity, the greater their interest will be !

Kids are naturally curious creatures, and once you have piqued their curiosity, you have also activated their observation, critical thinking, and experimentation skills.

These science activities are perfect for the senses, allowing play and exploration without adult-led directions. Kids will naturally start to pick up on the simple science concepts presented just by having a fun conversation about it all with you!

• 5 Senses Exploration Station
• 5 Senses Coloring Book (Free)
• 5 Senses Discovery Bags
• Apple Taste Test with the 5 Senses
• Pop Rocks and 5 Senses

Awesome Science Activities for Preschoolers

💡 Many of the preschool science projects below can be adapted to your kids’ current level. Also, these are perfect for kids of multiple ages to work together in small groups.

Click on each link below for full instructions. Also, look through our seasonal preschool science activities and grab the free science experiment cards!

• Spring Preschool Science Experiments
• Fall Preschool Science Experiments: apples and pumpkins
• Winter Preschool Science Experiments

Check out how different materials absorb water with this simple what absorbs water activity. 

ALKA SELTZER CHEMICAL REACTIONS

Make an Alka Seltzer Rocket , try a simple Alka Seltzer Experiment or a homemade Lava Lamp to check out this neat chemical reaction.

BAKING SODA AND VINEGAR EXPERIMENTS

Who doesn’t like a fizzing, foaming eruption? From an erupting lemon volcano to our simple baking soda balloon experiment. . Check out our list of baking soda science activities to get started!

BALLOON RACE CARS

Explore energy, measure distance, build different cars to explore speed and distance with simple balloon cars. You can use Duplo, LEGO, or build your own car.

BALLOON ROCKETS

Gas, energy, and power! Make Go power! Set up a simple balloon rocket. All you need is a string, a straw, and a balloon!

BURSTING BAGS

Definitely take this bursting bags science activity outside! Will it pop? This science activity will have you on the edge of your seat!

BUTTER IN A JAR

The science you can spread with a tasty homemade butter, after a good workout for the arms anyway!

BUTTERFLY LIFE CYCLE

Explore the butterfly life cycle with a free printable pack and butterfly sensory bin idea. Or create an edible butterfly life cycle instead!

Explore the simple fun of bubbles with these easy bubble experiments ! Can you make a bubble bounce? We have a recipe for the perfect bubble solution too.

Check out even more bubble fun with 2D bubble shapes or 3D bubble shapes , bubble painting , and bubble snakes !

BUILDING BRIDGES

This bridge building activity is an easy engineering project for young kids. It starts with the planning and designing process and ends with the building process. Construct your own bridge from simple supplies.

BUILDING TOWERS

Kids love building, and building structures is a great activity that incorporates many skills and is also frugal. Check out a variety of building activities .

CANDY SCIENCE

Play Willy Wonka for a day and explore candy science with floating m&m’s, chocolate slime, dissolving candy experiments, and more!

CELERY AND FOOD COLORING

Watch how water moves through a plant with a simple celery food coloring science experiment!

CHICK PEA FOAM

Have fun with this taste-safe sensory play foam made with ingredients you probably already have in the kitchen! This edible shaving foam, or aquafaba as it is commonly known, is made from the water chickpeas are cooked in.

COLOR MIXING

Color mixing is a science. Learn colors through play with these preschool color activities .

CRYSTAL GROWING

Growing crystals is simple! You can easily grow your own crystals at home or in the classroom with our simple recipe. Make a rainbow crystal , a snowflake , hearts , crystal eggshells , and even crystal seashells.

DENSITY {LIQUIDS}

Can one liquid be lighter than the other? Find out with this easy liquid density experiment!

DINOSAUR FOSSILS

Be a paleontologist for a day, make homemade dinosaur fossils , and then go on your own dig . Check out all our fun preschool dinosaur activities .

DISCOVERY BOTTLES

Science in a bottle. Explore all kinds of simple science ideas right in a bottle! They are perfect for themes, too, like these Earth Day ones!

Test how strong an egg is with this easy eggshell strength experiment. Find out what happens to an egg in vinegar , and try our mess-free egg drop challenge for young kids.

Have you ever changed the color of a flower? Try this color-changing flower science experiment and learn about how a flower works! Or why not try growing your own flowers with our list of easy flowers to grow .

What goes up must come down. Have young kids explore concepts in gravity around the house or classroom with simple objects you already have.

FIZZING LEMONADE

Explore the senses and a little chemistry with our fizzy lemonade recipe.

ICE CREAM IN A BAG

Homemade ice cream is yummy, edible science with only three ingredients! Don’t forget the winter gloves and sprinkles—this gets cold!

ICE MELT SCIENCE

An ice melt activity is a simple science you can set up in many different ways with many themes. Ice melting is a wonderful introduction to a simple science concept for young kids! Check out our list of ice activities for preschool.

Try the classic expanding ivory soap experiment! One bar of ivory soap can be very exciting! Also see how we experimented with one bar of soap and turned it into either soap foam!

Another must try science experiment using oil and water, a lava lamp experiment is always a favorite!

LETTUCE GROWING ACTIVITY

Set up a lettuce growing station. This is fascinating to watch and pretty quick to do. We watched the new lettuce grow taller each day!

Magic milk is one of our most popular science experiments. It is also just plain fun and mesmerizing!

What’s magnetic? What’s not magnetic? You can set up a magnet science discovery table for your kids to explore and this fun magnet activity !

MIRRORS AND REFLECTIONS

Mirrors are fascinating and have wonderful play and learning possibilities plus it makes for great science!

BOUNCY EGG EXPERIMENT

Ah, the egg in vinegar experiment. You need a little patience for this one {takes 7 days}, but the end result is really cool!

OOBLECK {NON-NEWTONIAN FLUIDS}

Oobleck is 2 ingredient fun! A simple recipe using kitchen cupboard ingredients, but it is the perfect example of a non-newtonian fluid. Also makes for fun sensory play. Make classic oobleck or colored oobleck.

Take the penny boat challenge and find out how many pennies your tin foil boat will hold before sinking. Learn about buoyancy and how boats float on water.

Make a simple pulley that really works, and test out lifting loads.

Learn about the science of rainbows as well as fun rainbow-themed science experiments. Check out our fun selection of simple-to-set-up  r ainbow science experiments . 

RAMPS AND FRICTION

We use cars and balls all the time with our rain gutters! Even flat pieces of wood or stiff cardboard work! Newton’s laws of motion really come alive with simple toy cars and homemade ramps.

ROCK CANDY (SUGAR CRYSTALS)

Another tasty science activity as you explore how sugar crystals form!

FOUR SEASONS

What’s the reason for the seasons, perfect for kids?

SEED GERMINATION

Our simple seed jar science activity is one of our most popular science activities for preschoolers. It is an excellent way to see how a seed grows!

THE 5 SENSES

Let’s explore the senses! Young kids are learning to use their senses every day. Set up a simple 5 Senses Science Table for exploring and learning how their senses work! Our candy taste test and senses activity are fun, too.

SHADOW SCIENCE

Explore shadows in several ways! Make shadows with your body (fun outdoor play and learning idea) and animal shadow puppets to check out!

Every kid should build a volcano with a simple chemical reaction, baking soda and vinegar! Build a sandbox volcano or a LEGO volcano ! Learn about fun volcano facts too!

Young kids learn by exploring, observing, and figuring out the way things work with hands-on activities. This volume activity encourages all of the above and is simple to set up.

WATER EXPERIMENTS

There are all kinds of fun science activities you can do with water. Use your STEM design skills to build your own water play wall , observe refraction of light in water , explore what dissolves in water or even try a simple solid liquid gas experiment .

WATER XYLOPHONE

Learn a little bit about the science of sound with a simple water xylophone.

WEATHER SCIENCE

Explore weather with rain clouds and tornados or even make a water cycle in a bottle!

TORNADO BOTTLE

Create a tornado in a bottle and study the weather safely!

We made both an indoor and outdoor zip line this year. Explore science concepts through play.

How to Teach Science to Preschoolers

Teaching science to preschoolers is a wonderful way to spark their curiosity about the world around them. Making the learning process fun, engaging, and age-appropriate is essential.

Make sure to check out…

• STEM for Toddlers
• STEM for Kindergarten
• STEM for Elementary

Begin by incorporating hands-on activities and experiments that encourage exploration and observation. Use simple and relatable examples from their everyday lives, such as observing plants grow, mixing colors, or exploring the properties of water.

Encourage questions and provide simple explanations that align with their level of understanding. Picture books can also be valuable tools for teaching science concepts. Add fun themes and colors for the holidays and seasons!

Additionally, fostering a sense of wonder and excitement about the natural world will lay a strong foundation for their future scientific learning. Remember that patience, repetition, and a nurturing environment are key to helping preschoolers develop a lifelong love for science.

Helpful Resources

Check out the links below to prepare yourself, your family, or your classroom for these easy preschool science experiments and activities. The key to success is in the preparation!

• Preschool Science Center Ideas
• Sensory Science Activities
• Fine Motor Activities
• Preschool Math Activities
• STEAM Books with Activities for Kids
• Make a homemade science kit that’s inexpensive!

Printable Preschool Pack

Get ready to explore this year with our growing Preschool STEM Bundle ! Or grab the individual packs.

What’s Included:

There are 12 fun preschool themes to get you started. This is an ” I can explore” series! You’ll find both seasonal and non-seasonal themes appropriate to preschool learning throughout the year.

Each unit contains approximately 15 activities, with instructions and templates  as needed. Hands-on activities are provided to keep it fun and exciting. This includes sensory bins, experiments, games, and more! Easy supplies keep it low-cost, and book suggestions add learning time.

22 Comments

Hi, this sounds like a neat activity for young toddlers to explore for texture. Could u please share the ingredients. My profession is in childcare. The children would like this activity I believe. Again, please share your slime recipe. Thank you!! LaTonia Jackson Armstrong

Hi! You simply need to click through the liquid starch slime link or get your recipe here box. However, I would suggest one of our taste safe slime recipes for toddlers as regular slime contains borax, sodium borate, or boric acid. These slimes should not be tasted!

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It’ fun for kids to experiment and science activities of any kind are a great way to encourage kids to explore. I will encourage my son about this hope so he will enjoy it. Thanks for the sharing such a interesting article.

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16 Integral Pros and Cons of Animal Experimentation

Over 25 million animals are used for experimentation in the U.S. every year. Monkeys, rabbits, cats, ferrets, pigs, sheep and chimpanzees are just some of the animals used for biomedical experiments, science education, and product and cosmetics testing. Some animals, however, are more preferred by laboratories. In fact, over 90% of test subjects are mice, birds and rats.

Many proponents of animal experimentation claim that the process is for a good cause. Better to use animals than humans for testing, right? After all, they are below humans in the natural chain of things. But, as English philosopher and social reformer Jeremy Bentham puts it, it is not a question of whether they can talk or reason, but whether or not they suffer. Considering that animals cry and show discomfort, it is safe to conclude that they feel something.

Unfortunately, vivisection, or the practice of animal experimentation, is perfectly acceptable and legal. It is the worst form of animal abuse that is institutionalized and sanctioned by our society. Despite the fact that the conditions of animals in labs are monotonous, stressful, and very unnatural for them, invasive experimentation persists, and even when the endpoint is death. Whether animal experimentation is good or bad really depends on who you are asking. But, if it is condone by society, then there must be some advantages to it, even if the benefits are at the expense of animal lives.

List of Pros of Animal Experimentation

1. Contributes to many cures and treatments that save many human lives The majority of the medical breakthroughs that have happened in the last 100 years were direct results from animal research and experimentation, according to the California Biomedical Research Association. Insulin, for example, was discovered through an experiment where dogs have their pancreases removed. The Anderson Cancer Center animal research also associated the vaccine for Hepatitis B with experimentation on chimpanzees. Without these experimentations, thousands, if not millions, of diabetic patients and those with hepatitis B would have been killed every year. The same facility also said that the chimps serve as humanity’s only hope for finding a Hepatitis C vaccine.

2. Provides adequate living, whole body system test subject No other living thing in this planet has the closest anatomical structure as humans than animals. A human body is extremely complex that cell cultures in a petri dish cannot provide sufficient test results or proof that a cure or product is effective. Testing a drug for side effects, for example, requires a circulatory system that will carry the drug to different organs. Studying interrelated processes is also best done in subjects with endocrine system, immune system, and central nervous system, something humans and animals have. What about the use of computer models? They would require accurate information that is gathered from animal research.

3. Humans and animals are almost identical in many ways The DNA of chimpanzees are 99% similar with humans, while the genetics of mice are 98% similar. Humans and animals are also biologically similar, having the same set of organs, bloodstream and central nervous system, which is why they are affected with the same diseases and health conditions. Given these circumstances, animals used in experimentation do serve as appropriate research subjects.

4. Provides an ethical alternative for testing Most people would say that it is unethical to use humans for invasive experimental procedures, especially when it can result in death. The lives of human volunteers must not be endangered when testing medicines for side effects or potential toxicity. Ethical consideration must also be made when genetic manipulation would be involved. Human trials must be preceded by animal testing, as stated by the World Medical Association Declaration of Helsinki. But, if animals could talk, they would probably demand the same ethical considerations.

5. Offer benefits to animals themselves Animal experimentation is not only beneficial to humans but animals as well. If the vaccines were not tested on them, a lot of them could have died from rabies, infectious hepatitis virus, anthrax, feline leukemia, and canine parvovirus. Remedies for hip dysplasia and glaucoma were also discovered through animal testing. But the real highlight is that vivisection helped kept endangered species, such as the California condor, the tamarins of Brazil, and the black-footed ferret, from becoming extinct. This is why animal testing is endorsed by the American Veterinary Medical Association.

6. Allow researchers to study a test subject for a whole life span Humans can live up to 80 years or more, which means some scientists would be dead before others results will be gathered. Laboratory mice, on the other hand, only live for 2 to 3 years, giving researchers an opportunity to study effects of genetic manipulation or treatments over an entire lifetime. In some cases, they can continue to study across several generations. This is why mice and rats have been used for long-term cancer research.

7. Animals are protected from abuse and mistreatment Contrary to what most opponents believe, animal research is highly regulated, with laws enacted to protect animals. Since 1966, the federal Animal Welfare Act have been regulating animal experimentation.

• Research animals must be provided with shelter that follows minimum housing standards, such as the right-sized enclosure, recommended temperature, access to clean food and water, etc.
• Veterinarians must regularly inspect the animals and their living conditions
• Each research facility must set up an Institutional Animal Care and Use Committee (IACUC) that will approve all proposals to use animals for experimentation.
• The IACUC will be responsible for enforcing humane treatment of animals.
• Research facilities funded by the US Public Health Service (PHS) must comply with the policies on Human Care and Use of Laboratory Animals imposed by PHS.

8. Fewer animals are used in research than as food for humans Compared to the amount of chicken, cattle, sheep and pigs that humans eat, relatively few of them are used in experimentation. With consideration to the medical progress and advancement such tests provided, it is a small price to pay. To illustrate, for every chicken used in research, an equivalent of 340 are used as food.

List of Cons of Animal Experimentation

1. Cruel and inhumane treatment Protocols in animal testing are often painful to the test subjects. They are forced fed, deprived of food and water, restrained physically for prolonged periods, inflicted with burns, wounds and pain to test for healing process effects and remedies, and even killed through neck-breaking or asphyxiation. This is according to the Humane Society International. When testing to evaluate irritation caused by cosmetics, for example, a rabbit’s eyes will be held open by clips so it cannot blink away the products being evaluated. The clips usually stay on for days, and to ensure the rabbits stay in place, they are incapacitated. Some experimentation also involves using lethal doses of certain chemicals to determine how much can kill animals.

2. Animals make poor test subjects This statement is a direct contradiction from what proponents believe about how closely related animals and humans are anatomically and biologically, because of the many metabolic, cellular, and anatomical differences between the two species. Using rats for toxicity, for example, must not be accepted as reliable since humans are nowhere close to being 70-kilogram rats, according to Thomas Hartung, professor of evidence-based toxicology at Johns Hopkins University. This is further supported by the 2013 study in the Archives of Toxicology that states that the lack of direct comparison of human data versus that of a mouse makes the usefulness of research data dubious.

3. Success in animal experimentation does not equate to human safety When the sleeping pill thalidomide was tested on pregnant rats, mice, cats and guinea pigs, there were no incidence of birth defects, except when administered at extremely high doses. However, when it was used by pregnant women, it resulted in severe deformities affecting 10,000 babies.

• The arthritis drug Vioxx, which turned out great on animals was really bad news on humans because it caused more than 20,000 heart attacks and sudden cardiac deaths.
• A majority of the drugs that passed animal tests, 94% to be exact, failed in human clinical trials.
• 100 of the drugs designed to treat stroke worked on animals, but completely failed in humans
• Over 85 vaccines for HIV worked well in primates, but failed in humans

4. Can lead to misleading research Some medicines and products that are harmful to animals are actually valuable to humans. Aspirin, for example, was almost shelved because it proved dangerous for animals. Imagine what would have happened if aspirin was completely taken off the pharmaceutical list? There would have been no way to lower the risk of organ transplant being rejected.

5. Most animals used in testing and research are not protected by the Animal Welfare Act (AWA) As of 2010, only over 1 million animals are covered by the AWA, leaving around 25 million more unprotected from mistreatment and abuse. These include birds, fish, mice and rats. And because vivisections within laboratory walls are regulated by the committee that the facility itself selected, animal subjects are even more at risk of being treated like prisoners in a hospital for their entire existence. One very good example of a clear violation of AWA was discovered in a federally funded facility in Louisiana, New Iberia Research Center (NIRC). The animals were so stressed out psychologically that they resorted to self-mutilation. The rest of the 337 violations that NIRC committed were caught on a video footage, showing the heartbreaking conditions of the animals. But this facility is just one of the many that violates AWA.

6. There are less expensive alternatives to animal experimentation Despite what proponents insist, cell cultures in a petri dish, or in vitro (in glass) testing, are not exactly useless or insufficient. They can even produce results that are more relevant than animal experimentation. The same thing is true when using artificial human skin as a test subject, instead of animal skin. Virtual reconstructions of human molecular structures done through computer models also have the capacity to predict toxicity levels of substances, so no need to poison animals to collect data and draw conclusions. And, when testing for adverse reactions, administering small doses on humans, also known as microdosing, also offers an alternative. Combined with blood analysis, results will be produced.

But what is really important is that these alternatives are less expensive than animal experimentations. In glass testing, for example, only costs $11,000, which is less than $21,000 than an “unscheduled DNA synthesis”. A phototoxicity test that doesn’t use rats only cost $1,300, which is almost $10,000 less than its animal-based equivalent. These only shows that animal tests are wasting plenty of government dollars allocated for research.

7. Plenty of animal lives are wasted Considering all the tests that failed, not to mention other non-experimental factors that affect animals, there is a significant number of animal lives wasted for nothing. They suffer or get killed during the experiment, and suffer the same fate after the experiment. But what is really inhumane and unethical are the poor research procedures used by some facilities. Serious flaws were discovered in plenty of studies in the UK and the U.S. that use rodents, according to a peer-reviewed study conducted in 2009. Selection bias was a major problem, but even with randomization and blinding technique used, proper selection of animals still failed. There is also a lack of hypothesis or objective related to the study.

8. Medical breakthroughs need not involve animals Is animal experimentation really that necessary in discovering treatments and cures? Opponents argue that there is really no evidence of its vital role in major medical advances. If funds and resources are focused on animal-free alternatives, more humane, ethical and inexpensive solutions. One such alternative that should be given full support is the microfluidic chip, also known as organs on a chip. This involves the use of chips to achieve certain functions of a human body, such as mix, pump and sort. The chips are lined with human cells so they work similar to human organs. With this alternative, researchers can no longer use the excuse that they need a living, whole-body system to run experiments.

Science and the scientific method: Definitions and examples

Here's a look at the foundation of doing science — the scientific method.

The scientific method

Hypothesis, theory and law, a brief history of science, additional resources, bibliography.

Science is a systematic and logical approach to discovering how things in the universe work. It is also the body of knowledge accumulated through the discoveries about all the things in the universe. 

The word "science" is derived from the Latin word "scientia," which means knowledge based on demonstrable and reproducible data, according to the Merriam-Webster dictionary . True to this definition, science aims for measurable results through testing and analysis, a process known as the scientific method. Science is based on fact, not opinion or preferences. The process of science is designed to challenge ideas through research. One important aspect of the scientific process is that it focuses only on the natural world, according to the University of California, Berkeley . Anything that is considered supernatural, or beyond physical reality, does not fit into the definition of science.

When conducting research, scientists use the scientific method to collect measurable, empirical evidence in an experiment related to a hypothesis (often in the form of an if/then statement) that is designed to support or contradict a scientific theory .

"As a field biologist, my favorite part of the scientific method is being in the field collecting the data," Jaime Tanner, a professor of biology at Marlboro College, told Live Science. "But what really makes that fun is knowing that you are trying to answer an interesting question. So the first step in identifying questions and generating possible answers (hypotheses) is also very important and is a creative process. Then once you collect the data you analyze it to see if your hypothesis is supported or not."

The steps of the scientific method go something like this, according to Highline College :

• Make an observation or observations.
• Form a hypothesis — a tentative description of what's been observed, and make predictions based on that hypothesis.
• Test the hypothesis and predictions in an experiment that can be reproduced.
• Analyze the data and draw conclusions; accept or reject the hypothesis or modify the hypothesis if necessary.
• Reproduce the experiment until there are no discrepancies between observations and theory. "Replication of methods and results is my favorite step in the scientific method," Moshe Pritsker, a former post-doctoral researcher at Harvard Medical School and CEO of JoVE, told Live Science. "The reproducibility of published experiments is the foundation of science. No reproducibility — no science."

Some key underpinnings to the scientific method:

• The hypothesis must be testable and falsifiable, according to North Carolina State University . Falsifiable means that there must be a possible negative answer to the hypothesis.
• Research must involve deductive reasoning and inductive reasoning . Deductive reasoning is the process of using true premises to reach a logical true conclusion while inductive reasoning uses observations to infer an explanation for those observations.
• An experiment should include a dependent variable (which does not change) and an independent variable (which does change), according to the University of California, Santa Barbara .
• An experiment should include an experimental group and a control group. The control group is what the experimental group is compared against, according to Britannica .

The process of generating and testing a hypothesis forms the backbone of the scientific method. When an idea has been confirmed over many experiments, it can be called a scientific theory. While a theory provides an explanation for a phenomenon, a scientific law provides a description of a phenomenon, according to The University of Waikato . One example would be the law of conservation of energy, which is the first law of thermodynamics that says that energy can neither be created nor destroyed. 

A law describes an observed phenomenon, but it doesn't explain why the phenomenon exists or what causes it. "In science, laws are a starting place," said Peter Coppinger, an associate professor of biology and biomedical engineering at the Rose-Hulman Institute of Technology. "From there, scientists can then ask the questions, 'Why and how?'"

Laws are generally considered to be without exception, though some laws have been modified over time after further testing found discrepancies. For instance, Newton's laws of motion describe everything we've observed in the macroscopic world, but they break down at the subatomic level.

This does not mean theories are not meaningful. For a hypothesis to become a theory, scientists must conduct rigorous testing, typically across multiple disciplines by separate groups of scientists. Saying something is "just a theory" confuses the scientific definition of "theory" with the layperson's definition. To most people a theory is a hunch. In science, a theory is the framework for observations and facts, Tanner told Live Science.

The earliest evidence of science can be found as far back as records exist. Early tablets contain numerals and information about the solar system , which were derived by using careful observation, prediction and testing of those predictions. Science became decidedly more "scientific" over time, however.

1200s: Robert Grosseteste developed the framework for the proper methods of modern scientific experimentation, according to the Stanford Encyclopedia of Philosophy. His works included the principle that an inquiry must be based on measurable evidence that is confirmed through testing.

1400s: Leonardo da Vinci began his notebooks in pursuit of evidence that the human body is microcosmic. The artist, scientist and mathematician also gathered information about optics and hydrodynamics.

1500s: Nicolaus Copernicus advanced the understanding of the solar system with his discovery of heliocentrism. This is a model in which Earth and the other planets revolve around the sun, which is the center of the solar system.

1600s: Johannes Kepler built upon those observations with his laws of planetary motion. Galileo Galilei improved on a new invention, the telescope, and used it to study the sun and planets. The 1600s also saw advancements in the study of physics as Isaac Newton developed his laws of motion.

1700s: Benjamin Franklin discovered that lightning is electrical. He also contributed to the study of oceanography and meteorology. The understanding of chemistry also evolved during this century as Antoine Lavoisier, dubbed the father of modern chemistry , developed the law of conservation of mass.

1800s: Milestones included Alessandro Volta's discoveries regarding electrochemical series, which led to the invention of the battery. John Dalton also introduced atomic theory, which stated that all matter is composed of atoms that combine to form molecules. The basis of modern study of genetics advanced as Gregor Mendel unveiled his laws of inheritance. Later in the century, Wilhelm Conrad Röntgen discovered X-rays , while George Ohm's law provided the basis for understanding how to harness electrical charges.

1900s: The discoveries of Albert Einstein , who is best known for his theory of relativity, dominated the beginning of the 20th century. Einstein's theory of relativity is actually two separate theories. His special theory of relativity, which he outlined in a 1905 paper, " The Electrodynamics of Moving Bodies ," concluded that time must change according to the speed of a moving object relative to the frame of reference of an observer. His second theory of general relativity, which he published as " The Foundation of the General Theory of Relativity ," advanced the idea that matter causes space to curve.

In 1952, Jonas Salk developed the polio vaccine , which reduced the incidence of polio in the United States by nearly 90%, according to Britannica . The following year, James D. Watson and Francis Crick discovered the structure of DNA , which is a double helix formed by base pairs attached to a sugar-phosphate backbone, according to the National Human Genome Research Institute .

2000s: The 21st century saw the first draft of the human genome completed, leading to a greater understanding of DNA. This advanced the study of genetics, its role in human biology and its use as a predictor of diseases and other disorders, according to the National Human Genome Research Institute .

• This video from City University of New York delves into the basics of what defines science.
• Learn about what makes science science in this book excerpt from Washington State University .
• This resource from the University of Michigan — Flint explains how to design your own scientific study.

Merriam-Webster Dictionary, Scientia. 2022. https://www.merriam-webster.com/dictionary/scientia

University of California, Berkeley, "Understanding Science: An Overview." 2022. ​​ https://undsci.berkeley.edu/article/0_0_0/intro_01  

Highline College, "Scientific method." July 12, 2015. https://people.highline.edu/iglozman/classes/astronotes/scimeth.htm  

North Carolina State University, "Science Scripts." https://projects.ncsu.edu/project/bio183de/Black/science/science_scripts.html  

University of California, Santa Barbara. "What is an Independent variable?" October 31,2017. http://scienceline.ucsb.edu/getkey.php?key=6045  

Encyclopedia Britannica, "Control group." May 14, 2020. https://www.britannica.com/science/control-group  

The University of Waikato, "Scientific Hypothesis, Theories and Laws." https://sci.waikato.ac.nz/evolution/Theories.shtml  

Stanford Encyclopedia of Philosophy, Robert Grosseteste. May 3, 2019. https://plato.stanford.edu/entries/grosseteste/  

Encyclopedia Britannica, "Jonas Salk." October 21, 2021. https://www.britannica.com/ biography /Jonas-Salk

National Human Genome Research Institute, "​Phosphate Backbone." https://www.genome.gov/genetics-glossary/Phosphate-Backbone  

National Human Genome Research Institute, "What is the Human Genome Project?" https://www.genome.gov/human-genome-project/What  

‌ Live Science contributor Ashley Hamer updated this article on Jan. 16, 2022.

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New development opens the door to more studies of protein movements

by Sara-Lena Brännström, Umea University

A new way to study protein movements has been developed by researchers at Umeå University and the MAX IV Laboratory in Lund. The method enables significantly more experiments than before and allows us to learn more about vital processes in the cells of humans, animals, and plants. The work is published in the journal Structure .

Proteins must move to perform their biological tasks in the cell. Such movements are called protein dynamics and are encoded in the protein's amino acid sequence through evolution. Since protein dynamics control essential processes like photosynthesis, nerve impulses, and energy conversion, many research groups are engaged in developing methods to study these structural changes at the molecular level.

One way is to use X-ray radiation from a synchrotron. The problem is that there are only a handful of synchrotron stations in the world that specialize in time-resolved experiments, which severely limits researchers' access.

Magnus Andersson's research group at Umeå University, together with a team from the MAX IV Laboratory led by Tomás Plivelic, has developed a method that instead combines fast X-ray detectors with indirect laser activation. This makes it possible to conduct time-resolved experiments at more synchrotron stations, including MAX IV in Sweden.

"Our experiment has great potential to pave way for a series of interesting studies of protein dynamics at the MAX IV synchrotron. For example, we would like to study how lipids in the cell membrane affect the dynamics of transport proteins that influence stress regulation in plants, as well as the heart's pump cycle," says Andersson, Associate Professor at the Department of Chemistry.

What the researchers have done is that instead of using specialized synchrotron stations, they used X-ray detectors that can register X-ray radiation on a micro- to millisecond time scale. These were combined with indirect laser activation of an ATP-dependent protein called adenylate kinase.

The reaction could be followed for 50 milliseconds with minimal negative effects from the X-ray radiation, which can otherwise break down biological material. For activation, an inactive form of ATP—a so-called caged compound—was used, which releases ATP when the laser hits the sample. This method enables studies of dynamics in a large number of proteins.

"An important aspect is that this project connects research in northern Sweden with national infrastructure in the southern parts of the country, which strengthens our research collaboration and enables further progress in the field," says Andersson.

Journal information: Structure

Provided by Umea University

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How Wombats May Save Other Animals From Wildfires

They build extensive burrow networks and don’t seem to mind when other woodland creatures use them as flameproof bunkers.

By Darren Incorvaia

It was a tale that seemed too good to be true, and in fact it was : During the devastating 2019-2020 Black Summer wildfires in southeastern Australia, false reports circulated that wombats had protected other animals by herding them into their burrows.

But like any good fairy tale, the reports did contain an inkling of truth; it turns out that wombat burrows do serve as fireproof refuges for small mammals, birds and reptiles during and after extreme fires.

Dale Nimmo, an ecologist at Charles Sturt University in Australia, was asked to fact-check the rumors of hero wombats that spread during the fires.

“In going through the literature,” Dr. Nimmo said, “there was quite a lot of evidence that species other than wombats were regularly using wombat burrows.”

While it turned out that wombats weren’t gallantly ushering woodland creatures out of harm’s way, Dr. Nimmo decided to explore the role of their burrows in forest ecosystems affected by a fire.

His colleague Grant Linley went searching for wombat burrows in Woomargama National Park and Woomargama State Forest. Around 70 of their combined 120 square miles had burned during the 2019-2020 fires. By the time Mr. Linley got there in the summer of 2021, vegetation had already started growing back.

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