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Hypothesis Testing | A Step-by-Step Guide with Easy Examples

Published on November 8, 2019 by Rebecca Bevans . Revised on June 22, 2023.

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics . It is most often used by scientists to test specific predictions, called hypotheses, that arise from theories.

There are 5 main steps in hypothesis testing:

• State your research hypothesis as a null hypothesis and alternate hypothesis (H o ) and (H a  or H 1 ).
• Collect data in a way designed to test the hypothesis.
• Perform an appropriate statistical test .
• Decide whether to reject or fail to reject your null hypothesis.
• Present the findings in your results and discussion section.

Though the specific details might vary, the procedure you will use when testing a hypothesis will always follow some version of these steps.

Table of contents

Step 1: state your null and alternate hypothesis, step 2: collect data, step 3: perform a statistical test, step 4: decide whether to reject or fail to reject your null hypothesis, step 5: present your findings, other interesting articles, frequently asked questions about hypothesis testing.

After developing your initial research hypothesis (the prediction that you want to investigate), it is important to restate it as a null (H o ) and alternate (H a ) hypothesis so that you can test it mathematically.

The alternate hypothesis is usually your initial hypothesis that predicts a relationship between variables. The null hypothesis is a prediction of no relationship between the variables you are interested in.

• H 0 : Men are, on average, not taller than women. H a : Men are, on average, taller than women.

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For a statistical test to be valid , it is important to perform sampling and collect data in a way that is designed to test your hypothesis. If your data are not representative, then you cannot make statistical inferences about the population you are interested in.

There are a variety of statistical tests available, but they are all based on the comparison of within-group variance (how spread out the data is within a category) versus between-group variance (how different the categories are from one another).

If the between-group variance is large enough that there is little or no overlap between groups, then your statistical test will reflect that by showing a low p -value . This means it is unlikely that the differences between these groups came about by chance.

Alternatively, if there is high within-group variance and low between-group variance, then your statistical test will reflect that with a high p -value. This means it is likely that any difference you measure between groups is due to chance.

Your choice of statistical test will be based on the type of variables and the level of measurement of your collected data .

• an estimate of the difference in average height between the two groups.
• a p -value showing how likely you are to see this difference if the null hypothesis of no difference is true.

Based on the outcome of your statistical test, you will have to decide whether to reject or fail to reject your null hypothesis.

In most cases you will use the p -value generated by your statistical test to guide your decision. And in most cases, your predetermined level of significance for rejecting the null hypothesis will be 0.05 – that is, when there is a less than 5% chance that you would see these results if the null hypothesis were true.

In some cases, researchers choose a more conservative level of significance, such as 0.01 (1%). This minimizes the risk of incorrectly rejecting the null hypothesis ( Type I error ).

The results of hypothesis testing will be presented in the results and discussion sections of your research paper , dissertation or thesis .

In the results section you should give a brief summary of the data and a summary of the results of your statistical test (for example, the estimated difference between group means and associated p -value). In the discussion , you can discuss whether your initial hypothesis was supported by your results or not.

In the formal language of hypothesis testing, we talk about rejecting or failing to reject the null hypothesis. You will probably be asked to do this in your statistics assignments.

However, when presenting research results in academic papers we rarely talk this way. Instead, we go back to our alternate hypothesis (in this case, the hypothesis that men are on average taller than women) and state whether the result of our test did or did not support the alternate hypothesis.

If your null hypothesis was rejected, this result is interpreted as “supported the alternate hypothesis.”

These are superficial differences; you can see that they mean the same thing.

You might notice that we don’t say that we reject or fail to reject the alternate hypothesis . This is because hypothesis testing is not designed to prove or disprove anything. It is only designed to test whether a pattern we measure could have arisen spuriously, or by chance.

If we reject the null hypothesis based on our research (i.e., we find that it is unlikely that the pattern arose by chance), then we can say our test lends support to our hypothesis . But if the pattern does not pass our decision rule, meaning that it could have arisen by chance, then we say the test is inconsistent with our hypothesis .

If you want to know more about statistics , methodology , or research bias , make sure to check out some of our other articles with explanations and examples.

• Normal distribution
• Descriptive statistics
• Measures of central tendency
• Correlation coefficient

Methodology

• Cluster sampling
• Stratified sampling
• Types of interviews
• Cohort study
• Thematic analysis

Research bias

• Implicit bias
• Cognitive bias
• Survivorship bias
• Availability heuristic
• Nonresponse bias
• Regression to the mean

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess — it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

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Statistics By Jim

Making statistics intuitive

Hypothesis Testing: Uses, Steps & Example

By Jim Frost 4 Comments

What is Hypothesis Testing?

Hypothesis testing in statistics uses sample data to infer the properties of a whole population . These tests determine whether a random sample provides sufficient evidence to conclude an effect or relationship exists in the population. Researchers use them to help separate genuine population-level effects from false effects that random chance can create in samples. These methods are also known as significance testing.

For example, researchers are testing a new medication to see if it lowers blood pressure. They compare a group taking the drug to a control group taking a placebo. If their hypothesis test results are statistically significant, the medication’s effect of lowering blood pressure likely exists in the broader population, not just the sample studied.

Using Hypothesis Tests

A hypothesis test evaluates two mutually exclusive statements about a population to determine which statement the sample data best supports. These two statements are called the null hypothesis and the alternative hypothesis . The following are typical examples:

• Null Hypothesis : The effect does not exist in the population.
• Alternative Hypothesis : The effect does exist in the population.

Hypothesis testing accounts for the inherent uncertainty of using a sample to draw conclusions about a population, which reduces the chances of false discoveries. These procedures determine whether the sample data are sufficiently inconsistent with the null hypothesis that you can reject it. If you can reject the null, your data favor the alternative statement that an effect exists in the population.

Statistical significance in hypothesis testing indicates that an effect you see in sample data also likely exists in the population after accounting for random sampling error , variability, and sample size. Your results are statistically significant when the p-value is less than your significance level or, equivalently, when your confidence interval excludes the null hypothesis value.

Conversely, non-significant results indicate that despite an apparent sample effect, you can’t be sure it exists in the population. It could be chance variation in the sample and not a genuine effect.

Learn more about Failing to Reject the Null .

5 Steps of Significance Testing

Hypothesis testing involves five key steps, each critical to validating a research hypothesis using statistical methods:

• Formulate the Hypotheses : Write your research hypotheses as a null hypothesis (H 0 ) and an alternative hypothesis (H A ).
• Data Collection : Gather data specifically aimed at testing the hypothesis.
• Conduct A Test : Use a suitable statistical test to analyze your data.
• Make a Decision : Based on the statistical test results, decide whether to reject the null hypothesis or fail to reject it.
• Report the Results : Summarize and present the outcomes in your report’s results and discussion sections.

While the specifics of these steps can vary depending on the research context and the data type, the fundamental process of hypothesis testing remains consistent across different studies.

Let’s work through these steps in an example!

Hypothesis Testing Example

Researchers want to determine if a new educational program improves student performance on standardized tests. They randomly assign 30 students to a control group , which follows the standard curriculum, and another 30 students to a treatment group, which participates in the new educational program. After a semester, they compare the test scores of both groups.

Download the CSV data file to perform the hypothesis testing yourself: Hypothesis_Testing .

The researchers write their hypotheses. These statements apply to the population, so they use the mu (μ) symbol for the population mean parameter .

• Null Hypothesis (H 0 ) : The population means of the test scores for the two groups are equal (μ 1 = μ 2 ).
• Alternative Hypothesis (H A ) : The population means of the test scores for the two groups are unequal (μ 1 ≠ μ 2 ).

Choosing the correct hypothesis test depends on attributes such as data type and number of groups. Because they’re using continuous data and comparing two means, the researchers use a 2-sample t-test .

Here are the results.

The treatment group’s mean is 58.70, compared to the control group’s mean of 48.12. The mean difference is 10.67 points. Use the test’s p-value and significance level to determine whether this difference is likely a product of random fluctuation in the sample or a genuine population effect.

Because the p-value (0.000) is less than the standard significance level of 0.05, the results are statistically significant, and we can reject the null hypothesis. The sample data provides sufficient evidence to conclude that the new program’s effect exists in the population.

Limitations

Hypothesis testing improves your effectiveness in making data-driven decisions. However, it is not 100% accurate because random samples occasionally produce fluky results. Hypothesis tests have two types of errors, both relating to drawing incorrect conclusions.

• Type I error: The test rejects a true null hypothesis—a false positive.
• Type II error: The test fails to reject a false null hypothesis—a false negative.

Learn more about Type I and Type II Errors .

Our exploration of hypothesis testing using a practical example of an educational program reveals its powerful ability to guide decisions based on statistical evidence. Whether you’re a student, researcher, or professional, understanding and applying these procedures can open new doors to discovering insights and making informed decisions. Let this tool empower your analytical endeavors as you navigate through the vast seas of data.

Learn more about the Hypothesis Tests for Various Data Types .

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Reader Interactions

June 10, 2024 at 10:51 am

Thank you, Jim, for another helpful article; timely too since I have started reading your new book on hypothesis testing and, now that we are at the end of the school year, my district is asking me to perform a number of evaluations on instructional programs. This is where my question/concern comes in. You mention that hypothesis testing is all about testing samples. However, I use all the students in my district when I make these comparisons. Since I am using the entire “population” in my evaluations (I don’t select a sample of third grade students, for example, but I use all 700 third graders), am I somehow misusing the tests? Or can I rest assured that my district’s student population is only a sample of the universal population of students?

June 10, 2024 at 1:50 pm

I hope you are finding the book helpful!

Yes, the purpose of hypothesis testing is to infer the properties of a population while accounting for random sampling error.

In your case, it comes down to how you want to use the results. Who do you want the results to apply to?

If you’re summarizing the sample, looking for trends and patterns, or evaluating those students and don’t plan to apply those results to other students, you don’t need hypothesis testing because there is no sampling error. They are the population and you can just use descriptive statistics. In this case, you’d only need to focus on the practical significance of the effect sizes.

On the other hand, if you want to apply the results from this group to other students, you’ll need hypothesis testing. However, there is the complicating issue of what population your sample of students represent. I’m sure your district has its own unique characteristics, demographics, etc. Your district’s students probably don’t adequately represent a universal population. At the very least, you’d need to recognize any special attributes of your district and how they could bias the results when trying to apply them outside the district. Or they might apply to similar districts in your region.

However, I’d imagine your 3rd graders probably adequately represent future classes of 3rd graders in your district. You need to be alert to changing demographics. At least in the short run I’d imagine they’d be representative of future classes.

Think about how these results will be used. Do they just apply to the students you measured? Then you don’t need hypothesis tests. However, if the results are being used to infer things about other students outside of the sample, you’ll need hypothesis testing along with considering how well your students represent the other students and how they differ.

I hope that helps!

June 10, 2024 at 3:21 pm

Thank you so much, Jim, for the suggestions in terms of what I need to think about and consider! You are always so clear in your explanations!!!!

June 10, 2024 at 3:22 pm

You’re very welcome! Best of luck with your evaluations!

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Hypothesis Testing – A Deep Dive into Hypothesis Testing, The Backbone of Statistical Inference

• September 21, 2023

Explore the intricacies of hypothesis testing, a cornerstone of statistical analysis. Dive into methods, interpretations, and applications for making data-driven decisions.

In this Blog post we will learn:

• What is Hypothesis Testing?
• Steps in Hypothesis Testing 2.1. Set up Hypotheses: Null and Alternative 2.2. Choose a Significance Level (α) 2.3. Calculate a test statistic and P-Value 2.4. Make a Decision
• Example : Testing a new drug.
• Example in python

1. What is Hypothesis Testing?

In simple terms, hypothesis testing is a method used to make decisions or inferences about population parameters based on sample data. Imagine being handed a dice and asked if it’s biased. By rolling it a few times and analyzing the outcomes, you’d be engaging in the essence of hypothesis testing.

Think of hypothesis testing as the scientific method of the statistics world. Suppose you hear claims like “This new drug works wonders!” or “Our new website design boosts sales.” How do you know if these statements hold water? Enter hypothesis testing.

2. Steps in Hypothesis Testing

• Set up Hypotheses : Begin with a null hypothesis (H0) and an alternative hypothesis (Ha).
• Choose a Significance Level (α) : Typically 0.05, this is the probability of rejecting the null hypothesis when it’s actually true. Think of it as the chance of accusing an innocent person.
• Calculate Test statistic and P-Value : Gather evidence (data) and calculate a test statistic.
• p-value : This is the probability of observing the data, given that the null hypothesis is true. A small p-value (typically ≤ 0.05) suggests the data is inconsistent with the null hypothesis.
• Decision Rule : If the p-value is less than or equal to α, you reject the null hypothesis in favor of the alternative.

2.1. Set up Hypotheses: Null and Alternative

Before diving into testing, we must formulate hypotheses. The null hypothesis (H0) represents the default assumption, while the alternative hypothesis (H1) challenges it.

For instance, in drug testing, H0 : “The new drug is no better than the existing one,” H1 : “The new drug is superior .”

2.2. Choose a Significance Level (α)

When You collect and analyze data to test H0 and H1 hypotheses. Based on your analysis, you decide whether to reject the null hypothesis in favor of the alternative, or fail to reject / Accept the null hypothesis.

The significance level, often denoted by $α$, represents the probability of rejecting the null hypothesis when it is actually true.

In other words, it’s the risk you’re willing to take of making a Type I error (false positive).

Type I Error (False Positive) :

• Symbolized by the Greek letter alpha (α).
• Occurs when you incorrectly reject a true null hypothesis . In other words, you conclude that there is an effect or difference when, in reality, there isn’t.
• The probability of making a Type I error is denoted by the significance level of a test. Commonly, tests are conducted at the 0.05 significance level , which means there’s a 5% chance of making a Type I error .
• Commonly used significance levels are 0.01, 0.05, and 0.10, but the choice depends on the context of the study and the level of risk one is willing to accept.

Example : If a drug is not effective (truth), but a clinical trial incorrectly concludes that it is effective (based on the sample data), then a Type I error has occurred.

Type II Error (False Negative) :

• Symbolized by the Greek letter beta (β).
• Occurs when you accept a false null hypothesis . This means you conclude there is no effect or difference when, in reality, there is.
• The probability of making a Type II error is denoted by β. The power of a test (1 – β) represents the probability of correctly rejecting a false null hypothesis.

Example : If a drug is effective (truth), but a clinical trial incorrectly concludes that it is not effective (based on the sample data), then a Type II error has occurred.

Balancing the Errors :

In practice, there’s a trade-off between Type I and Type II errors. Reducing the risk of one typically increases the risk of the other. For example, if you want to decrease the probability of a Type I error (by setting a lower significance level), you might increase the probability of a Type II error unless you compensate by collecting more data or making other adjustments.

It’s essential to understand the consequences of both types of errors in any given context. In some situations, a Type I error might be more severe, while in others, a Type II error might be of greater concern. This understanding guides researchers in designing their experiments and choosing appropriate significance levels.

2.3. Calculate a test statistic and P-Value

Test statistic : A test statistic is a single number that helps us understand how far our sample data is from what we’d expect under a null hypothesis (a basic assumption we’re trying to test against). Generally, the larger the test statistic, the more evidence we have against our null hypothesis. It helps us decide whether the differences we observe in our data are due to random chance or if there’s an actual effect.

P-value : The P-value tells us how likely we would get our observed results (or something more extreme) if the null hypothesis were true. It’s a value between 0 and 1. – A smaller P-value (typically below 0.05) means that the observation is rare under the null hypothesis, so we might reject the null hypothesis. – A larger P-value suggests that what we observed could easily happen by random chance, so we might not reject the null hypothesis.

2.4. Make a Decision

Relationship between $α$ and P-Value

When conducting a hypothesis test:

We then calculate the p-value from our sample data and the test statistic.

Finally, we compare the p-value to our chosen $α$:

• If $p−value≤α$: We reject the null hypothesis in favor of the alternative hypothesis. The result is said to be statistically significant.
• If $p−value>α$: We fail to reject the null hypothesis. There isn’t enough statistical evidence to support the alternative hypothesis.

3. Example : Testing a new drug.

Imagine we are investigating whether a new drug is effective at treating headaches faster than drug B.

Setting Up the Experiment : You gather 100 people who suffer from headaches. Half of them (50 people) are given the new drug (let’s call this the ‘Drug Group’), and the other half are given a sugar pill, which doesn’t contain any medication.

• Set up Hypotheses : Before starting, you make a prediction:
• Null Hypothesis (H0): The new drug has no effect. Any difference in healing time between the two groups is just due to random chance.
• Alternative Hypothesis (H1): The new drug does have an effect. The difference in healing time between the two groups is significant and not just by chance.

Calculate Test statistic and P-Value : After the experiment, you analyze the data. The “test statistic” is a number that helps you understand the difference between the two groups in terms of standard units.

For instance, let’s say:

• The average healing time in the Drug Group is 2 hours.
• The average healing time in the Placebo Group is 3 hours.

The test statistic helps you understand how significant this 1-hour difference is. If the groups are large and the spread of healing times in each group is small, then this difference might be significant. But if there’s a huge variation in healing times, the 1-hour difference might not be so special.

Imagine the P-value as answering this question: “If the new drug had NO real effect, what’s the probability that I’d see a difference as extreme (or more extreme) as the one I found, just by random chance?”

For instance:

• P-value of 0.01 means there’s a 1% chance that the observed difference (or a more extreme difference) would occur if the drug had no effect. That’s pretty rare, so we might consider the drug effective.
• P-value of 0.5 means there’s a 50% chance you’d see this difference just by chance. That’s pretty high, so we might not be convinced the drug is doing much.
• If the P-value is less than ($α$) 0.05: the results are “statistically significant,” and they might reject the null hypothesis , believing the new drug has an effect.
• If the P-value is greater than ($α$) 0.05: the results are not statistically significant, and they don’t reject the null hypothesis , remaining unsure if the drug has a genuine effect.

4. Example in python

For simplicity, let’s say we’re using a t-test (common for comparing means). Let’s dive into Python:

Making a Decision : “The results are statistically significant! p-value < 0.05 , The drug seems to have an effect!” If not, we’d say, “Looks like the drug isn’t as miraculous as we thought.”

5. Conclusion

Hypothesis testing is an indispensable tool in data science, allowing us to make data-driven decisions with confidence. By understanding its principles, conducting tests properly, and considering real-world applications, you can harness the power of hypothesis testing to unlock valuable insights from your data.

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Hypothesis Testing

Measuring the consistency between a model and data

C lassical statistics features two primary methods for using a sample of data to make an inference about a more general process. The first is the confidence interval, which expresses the uncertainty in an estimate of a population parameter. The second classical method of generalization is the hypothesis test.

The hypothesis test takes a more active approach to reasoning: it posits a specific explanation for how the data could be generated, then evaluates whether or not the observed data is consistent with that model. The hypothesis test is one of the most common statistical tools in the social and natural sciences, but the reasoning involved can be counter-intuitive. Let’s introduce the logic of a hypothesis test by looking at another criminal case that drew statisticians into the mix.

Example: The United States vs Kristen Gilbert

In 1989, fresh out of nursing school, Kristen Gilbert got a job at the VA Medical Center in Northampton, Massachusetts, not far from where she grew up 1 . Within a few years, she became admired for her skill and competence.

Gilbert’s skill was on display whenever a “code blue” alarm was sounded. This alarm indicates that a patient has gone into cardiac arrest and must be addressed quickly by administering a shot of epinephrine to restart the heart. Gilbert developed for a reputation for her steady hand in these crises.

By the mid-1990s, however, the other nurses started to grow suspicious. There seemed to be a few too many code blues, and a few too many deaths, during Gilbert’s shifts. The staff brought their concerns to the VA administration, who brought in a statistician to evaluate the data.

The data that the VA provided to the statistician contained the number of deaths at the medical center over the previous 10 years, broken out by the three shifts of the days: night, daytime, and evening. As part of the process of exploratory data analysis, the statistician constructed a plot.

This visualization reveals several striking trends. Between 1990 and 1995, there were dramatically more deaths than the years before and after that interval. Within that time span, it was the evening shift that had most of the deaths. The exception is 1990, when the night and daytime shifts had the most deaths.

So when was Gilbert working? She began working in this part of the hospital in March 1990 and stopped working in February 1996. Her shifts throughout that time span? The evening shifts. The one exception was 1990, when she was assigned to work the night shift.

This evidence is compelling in establishing an association between Gilbert and the increase in deaths. When the district attorney brought a case against Gilbert in court, this was the first line of evidence they provided. In a trial, however, there is a high burden of proof.

Could there be an alternative explanation for the trend found in this data?

The role of random chance

Suppose for a moment that the occurrence of deaths at the hospital had nothing to do with Gilbert being on shift. In that case we would expect that the proportion of shifts with a death would be fairly similar when comparing shifts where Gilbert was working and shifts where she was not. But we wouldn’t expect those proportions to be exactly equal. It’s reasonable to think that a slightly higher proportion of Gilbert’s shifts could have had a death just due to random chance, not due to anything malicious on her part.

So just how different were these proportions in the data? The plot above shows data from 1,641 individual shifts, on which three different variables were recorded: the shift number, whether or not there was a death on the shift, and whether or not Gilbert was working that shift.

Here are the first 10 observations.

Using this data frame, we can calculate the sample proportion of shifts where Gilbert was working (257) that had a death (40) and compare them to the sample proportion of shifts where Gilbert was not working (1384) that had a death (34).

\[ \hat{p}_{gilbert} - \hat{p}_{no\_gilbert} = \frac{40}{257} - \frac{34}{1384} = .155 - .024 = .131 \]

A note on notation: it’s common to use \(\hat{p}\) (“p hat”) to indicate that a proportion has been computed from a sample of data.

A difference of .131 seems dramatic, but is that within the bounds of what we might expect just due to chance? One way to address this question is to phrase it as: if in fact the probability of a death on a given shift is independent of whether or not Gilbert is on the shift, what values would we expect for the difference in observed proportions?

We can answer this question by using simulation. To a simulate a world in which deaths are independent of Gilbert, we can

• Shuffle (or permute) the values in the death variable in the data frame to break the link between that variable and the staff variable.
• Calculate the resulting difference in proportion of deaths in each group.

The rationale for shuffling values in one of the columns is that if in fact those two columns are independent of one another, then it was just random chance that led to a value of one variable landing in the same row as the value of the other variable. It could just as well have been a different pairing. Shuffling captures another example of the arbitrary pairings that we could have observed if the two variables were independent of one another 2 .

By repeating steps 1 and 2 many many times, we can build up the full distribution of the values that this difference in proportions could take.

As expected, in a world where these two variables are independent of one another, we would expect a difference in proportions around zero. Sometimes, however, that statistic might reach values of +/- .01 or .02 or rarely .03. In the 500 simulated statistics shown above, however, none of them reached beyond +/- .06.

So if that’s the range of statistics we would expect in a world where random chance is the only mechanism driving the difference in proportions, how does it compare to the world that we actually observed? The statistic that we observed in the data was .131, more than twice the value of the most extreme statistic observed above.

To put that into perspective, we can plot the observed statistic as a vertical line on the same plot.

The method used above shows that the chance of observing a difference of .131 is incredibly unlikely if in fact deaths were independent of Gilbert being on shift. On this point, the statisticians on the case agreed that they could rule out random chance as an explanation for this difference. Something else must have been happening.

Elements of a Hypothesis Test

The logic used by the statisticians in the Gilbert case is an example of a hypothesis test. There are a few key components common to every hypothesis test, so we’ll lay them out one-by-one.

A hypothesis test begins with the assertion of a null hypothesis.

It is common for the null hypothesis to be that nothing interesting is happening or that it is business as usual, a hypothesis that statisticians try to refute with data. In Gilbert case, this could be described as “The occurrence of a death is independence of the presence of Gilbert” or “The probability of death is the same whether or not Gilbert is on shift” or “The difference in the probability of death is zero, when comparing shifts where Gilbert is present to shifts where Gilbert is not present”. Importantly, the null model describes a possible state of the world, therefore the latter two versions are framed in terms of parameters ( \(p\) for proportions) instead of observed statistics ( \(\hat{p}\) ).

The hypothesis that something indeed is going on is usually framed as the alternative hypothesis.

In the Gilbert case, the corresponding alternative hypothesis is that there is “The occurrence of a death is dependent on the presence of Gilbert” or “The probability of death is different whether or not Gilbert is on shift” or “The difference in the probability of death is non-zero , when comparing shifts where Gilbert is present to shifts where Gilbert is not present”

In order to determine whether the observed data is consistent with the null hypothesis, it is necessary to compress the data down into a single statistic.

In Gilbert’s case, a difference in two proportions, \(\hat{p}_1 - \hat{p}_2\) is a natural test statistic and the observed test statistic was .131.

It’s not enough, though, to just compute the observed statistic. We need to know how likely this statistic would be in a world where the null hypothesis is true. This probability is captured in the notion of a p-value.

If the p-value is high, then the data is consistent with the null hypothesis. If the p-value is very low, however, there the statistic that was observed would be very unlikely in a world where the null hypothesis was true. As a consequence, the null hypothesis can be rejected as reasonable model for the data.

The p-value can be estimated using the proportion of statistics from the simulated null distribution that are as or more extreme than the observed statistic. In the simulation for the Gilbert case, there were 0 statistics greater than .131, so the estimated p-value is zero.

What a p-value is not

The p-value has been called the most used as well as the most abused tool in statistics. Here are three common misinterpretations to be wary of.

The p-value is the probability that the null hypothesis is true (FALSE!)

This is one of the most common confusions about p-values. Graphically, a p-value corresponds to the area in the tail of the null distribution that is more extreme than the observed test statistic. That null distribution can only be created if you assume that the null hypothesis is true. The p-value is fundamentally a conditional probability of observing the statistic (or more extreme) given the null hypothesis is true. It is flawed reasoning to start with an assumption that the null hypothesis is true and arrive at a probability of that same assumption.

A very high p-value suggests that the null hypothesis is true (FALSE!)

This interpretation is related to the first one but can lead to particularly wrongheaded decisions. One way to keep your interpretation of a p-value straight is to recall the distinction made in the US court system. A trial proceeds under the assumption that the defendant is innocent. The prosecution presents evidence of guilt. If the evidence is convincing the jury will render a verdict of “guilty”. If the evidence is not-convincing (that is, the p-value is high) then the jury will render a verdict of “not guilty” - not a verdict of “innocent”.

Imagine a setting where the prosecution has presented no evidence at all. That by no means indicates that the defendant is innocent, just that there was insufficient evidence to establish guilt.

The p-value is the probability of the data (FALSE!)

This statement has a semblance of truth to it but is missing an important qualifier. The probability is calculated based on the null distribution, which requires the assumption that the null hypothesis is true. It’s also not quite specific enough. Most often p-values are calculated as probabilities of test statistics, not probabilities of the full data sets.

Another more basic check on your understanding of a p-value: a p-value is a (conditional) probability, therefore it must between a number between 0 and 1. If you ever find yourself computing a p-value of -6 or 3.2, be sure to pause and revisit your calculations!

One test, many variations

The hypothesis testing framework laid out above is far more general than just this particular example from the case of Kristen Gilbert where we computed a difference in proportions and used shuffling (aka permutation) to build the null distribution. Below are just a few different research questions that could be addressed using a hypothesis test.

Pollsters have surveyed a sample of 200 voters ahead of an election to assess their relative support for the Republican and Democratic candidate. The observed difference in those proportions is .02. Is this consistent with the notion of evenly split support for the two candidates, or is one decidedly in the lead?

Brewers have tapped 7 barrels of beer and measured the average level of a compound related to the acidity of the beer as 610 parts per million. The acceptable level for this compound is 500 parts per million. Is this average of 610 consistent with the notion that the average of the whole batch of beer (many hundreds of barrels) is at the acceptable level of this compound?

A random sample of 40 users of a food delivery app were randomly assigned two different versions of a menu where they entered the amount of their tip: one with the tip amount in ascending order, the other in descending order. The average tip amount of those with the menu in ascending order was found to be $3.87 while the average tip of the users in the descending order group was $3.96. Could this difference in averages be explained by chance?

Although the contexts of these problems are very different, as are the types of statistics they’ve calculated, they can still be characterized as a hypothesis test by asking the following questions:

What is the null hypothesis used by the researchers?

What is the value of the observed test statistic?

How did researchers approximate the null distribution?

What was the p-value, what does it tell us and what does it not tell us?

In classical statistics there are two primary tools for assessing the role that random variability plays in the data that you have observed. The first is the confidence interval, which quantifies the amount of uncertainty in a point estimate due to the variability inherent in drawing a small random sample from a population. The second is the hypothesis test, which postings a specific model by which the data could be generated, then assesses the degree to which the observed data is consistent with that model.

The hypothesis test begins with the assertion of a null hypothesis that describes a chance mechanism for generating data. A test statistic is then selected that corresponds to that null hypothesis. From there, the sampling distribution of that statistic under the null hypothesis is approximated through a computational method (such as using permutation, as shown here) or one rooted in probability theory (such as the Central Limit Theorem). The final result of the hypothesis test procedure is the p-value, which is approximated as the proportion of the null distribution that is as or more extreme than the observed test statistic. The p-value measures the consistency between the null hypothesis and the observed test statistic and should be interpreted carefully.

A postscript on the case of Kristen Gilbert. Although the hypothesis test ruled out random chance as the reason for the spike in deaths under her watch, it didn’t rule out other potential causes for that spike. It’s possible, after all, that the nightshifts that Gilbert was working happen to be the time of day when cardiac arrests are more common. For this reason, the statistical evidence was never presented to the jury, but the jury nonetheless found her guilty based on other evidence presented in the trial.

The Ideas in Code

A hypothesis test using permutation can be implemented by introducing one new step into the process used for calculating a bootstrap interval. The key distinction is that in a hypothesis test the researchers puts forth a model for how the data could be generated. That is the role of hypothesize() .

hypothesize()

A function to place before generate() in an infer pipeline where you can specify a null model under which to generate data. The one necessary argument is

• null : the null hypothesis. Options include "independence" and "point" .

The following example implements a permutation test under the null hypothesis that there is no relationship between the body mass of penguins and their

• The output is the original data frame with new information appended to describe what the null hypothesis is for this data set.
• There are other forms of hypothesis tests that you will see involving a "point" null hypothesis. Those require adding additional arguments to hypothesize() .

Calculating an observed statistic

Let’s say for this example you select as your test statistic a difference in means, \(\bar{x}_{female} - \bar{x}_{male}\) . While you can use tools you know - group_by() and summarize() to calculate this statistic, you can also recycle much of the code that you’ll use to build the null distribution with infer .

Calculating the null distribution

To generate a null distribution of the kind of differences in means that you’d observe in a world where body mass had nothing to do with sex, just add the hypothesis with hypothesize() and the generation mechanism with generate() .

• The output data frame has reps rows and 2 columns: one indicating the replicate and the other with the statistic (a difference in means).

This case study appears in Statistics in the Courtroom: United States v. Kristen Gilbert by Cobb and Gelbach, published in Statistics: A Guide to the Unknown by Peck et. al. ↩︎

The technical notion that motivates the use of shuffling is a slightly more general notion than independence called exchangability. The distinction between these two related concepts is a topic in a course in probability. ↩︎

Hypothesis Testing

About hypothesis testing.

Contents (Click to skip to the section):

What is a Hypothesis?

What is hypothesis testing.

• Hypothesis Testing Examples (One Sample Z Test).
• Hypothesis Test on a Mean (TI 83).

Bayesian Hypothesis Testing.

• More Hypothesis Testing Articles
• Hypothesis Tests in One Picture
• Critical Values

What is the Null Hypothesis?

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A hypothesis is an educated guess about something in the world around you. It should be testable, either by experiment or observation. For example:

• A new medicine you think might work.
• A way of teaching you think might be better.
• A possible location of new species.
• A fairer way to administer standardized tests.

It can really be anything at all as long as you can put it to the test.

What is a Hypothesis Statement?

If you are going to propose a hypothesis, it’s customary to write a statement. Your statement will look like this: “If I…(do this to an independent variable )….then (this will happen to the dependent variable ).” For example:

• If I (decrease the amount of water given to herbs) then (the herbs will increase in size).
• If I (give patients counseling in addition to medication) then (their overall depression scale will decrease).
• If I (give exams at noon instead of 7) then (student test scores will improve).
• If I (look in this certain location) then (I am more likely to find new species).

A good hypothesis statement should:

• Include an “if” and “then” statement (according to the University of California).
• Include both the independent and dependent variables.
• Be testable by experiment, survey or other scientifically sound technique.
• Be based on information in prior research (either yours or someone else’s).
• Have design criteria (for engineering or programming projects).

Hypothesis testing can be one of the most confusing aspects for students, mostly because before you can even perform a test, you have to know what your null hypothesis is. Often, those tricky word problems that you are faced with can be difficult to decipher. But it’s easier than you think; all you need to do is:

• Figure out your null hypothesis,
• State your null hypothesis,
• Choose what kind of test you need to perform,
• Either support or reject the null hypothesis .

If you trace back the history of science, the null hypothesis is always the accepted fact. Simple examples of null hypotheses that are generally accepted as being true are:

• DNA is shaped like a double helix.
• There are 8 planets in the solar system (excluding Pluto).
• Taking Vioxx can increase your risk of heart problems (a drug now taken off the market).

How do I State the Null Hypothesis?

You won’t be required to actually perform a real experiment or survey in elementary statistics (or even disprove a fact like “Pluto is a planet”!), so you’ll be given word problems from real-life situations. You’ll need to figure out what your hypothesis is from the problem. This can be a little trickier than just figuring out what the accepted fact is. With word problems, you are looking to find a fact that is nullifiable (i.e. something you can reject).

Hypothesis Testing Examples #1: Basic Example

A researcher thinks that if knee surgery patients go to physical therapy twice a week (instead of 3 times), their recovery period will be longer. Average recovery times for knee surgery patients is 8.2 weeks.

The hypothesis statement in this question is that the researcher believes the average recovery time is more than 8.2 weeks. It can be written in mathematical terms as: H 1 : μ > 8.2

Next, you’ll need to state the null hypothesis .  That’s what will happen if the researcher is wrong . In the above example, if the researcher is wrong then the recovery time is less than or equal to 8.2 weeks. In math, that’s: H 0 μ ≤ 8.2

Rejecting the null hypothesis

Ten or so years ago, we believed that there were 9 planets in the solar system. Pluto was demoted as a planet in 2006. The null hypothesis of “Pluto is a planet” was replaced by “Pluto is not a planet.” Of course, rejecting the null hypothesis isn’t always that easy— the hard part is usually figuring out what your null hypothesis is in the first place.

Hypothesis Testing Examples (One Sample Z Test)

The one sample z test isn’t used very often (because we rarely know the actual population standard deviation ). However, it’s a good idea to understand how it works as it’s one of the simplest tests you can perform in hypothesis testing. In English class you got to learn the basics (like grammar and spelling) before you could write a story; think of one sample z tests as the foundation for understanding more complex hypothesis testing. This page contains two hypothesis testing examples for one sample z-tests .

One Sample Hypothesis Testing Example: One Tailed Z Test

A principal at a certain school claims that the students in his school are above average intelligence. A random sample of thirty students IQ scores have a mean score of 112.5. Is there sufficient evidence to support the principal’s claim? The mean population IQ is 100 with a standard deviation of 15.

Step 1: State the Null hypothesis . The accepted fact is that the population mean is 100, so: H 0 : μ = 100.

Step 2: State the Alternate Hypothesis . The claim is that the students have above average IQ scores, so: H 1 : μ > 100. The fact that we are looking for scores “greater than” a certain point means that this is a one-tailed test.

Step 4: State the alpha level . If you aren’t given an alpha level , use 5% (0.05).

Step 5: Find the rejection region area (given by your alpha level above) from the z-table . An area of .05 is equal to a z-score of 1.645.

Step 6: If Step 6 is greater than Step 5, reject the null hypothesis. If it’s less than Step 5, you cannot reject the null hypothesis. In this case, it is more (4.56 > 1.645), so you can reject the null.

One Sample Hypothesis Testing Examples: #3

Blood glucose levels for obese patients have a mean of 100 with a standard deviation of 15. A researcher thinks that a diet high in raw cornstarch will have a positive or negative effect on blood glucose levels. A sample of 30 patients who have tried the raw cornstarch diet have a mean glucose level of 140. Test the hypothesis that the raw cornstarch had an effect.

• State the null hypothesis : H 0 :μ=100
• State the alternate hypothesis : H 1 :≠100
• State your alpha level. We’ll use 0.05 for this example. As this is a two-tailed test, split the alpha into two. 0.05/2=0.025
• Find the z-score associated with your alpha level . You’re looking for the area in one tail only . A z-score for 0.75(1-0.025=0.975) is 1.96. As this is a two-tailed test, you would also be considering the left tail (z = 1.96)
•   If Step 5 is less than -1.96 or greater than 1.96 (Step 3), reject the null hypothesis . In this case, it is greater, so you can reject the null.

*This process is made much easier if you use a TI-83 or Excel to calculate the z-score (the “critical value”). See:

• Critical z value TI 83
• Z Score in Excel

Hypothesis Testing Examples: Mean (Using TI 83)

You can use the TI 83 calculator for hypothesis testing, but the calculator won’t figure out the null and alternate hypotheses; that’s up to you to read the question and input it into the calculator.

Example problem : A sample of 200 people has a mean age of 21 with a population standard deviation (σ) of 5. Test the hypothesis that the population mean is 18.9 at α = 0.05.

Step 1: State the null hypothesis. In this case, the null hypothesis is that the population mean is 18.9, so we write: H 0 : μ = 18.9

Step 2: State the alternative hypothesis. We want to know if our sample, which has a mean of 21 instead of 18.9, really is different from the population, therefore our alternate hypothesis: H 1 : μ ≠ 18.9

Step 3: Press Stat then press the right arrow twice to select TESTS.

Step 4: Press 1 to select 1:Z-Test… . Press ENTER.

Step 5: Use the right arrow to select Stats .

Step 6: Enter the data from the problem: μ 0 : 18.9 σ: 5 x : 21 n: 200 μ: ≠μ 0

Step 7: Arrow down to Calculate and press ENTER. The calculator shows the p-value: p = 2.87 × 10 -9

This is smaller than our alpha value of .05. That means we should reject the null hypothesis .

Bayesian Hypothesis Testing: What is it?

Bayesian hypothesis testing helps to answer the question: Can the results from a test or survey be repeated? Why do we care if a test can be repeated? Let’s say twenty people in the same village came down with leukemia. A group of researchers find that cell-phone towers are to blame. However, a second study found that cell-phone towers had nothing to do with the cancer cluster in the village. In fact, they found that the cancers were completely random. If that sounds impossible, it actually can happen! Clusters of cancer can happen simply by chance . There could be many reasons why the first study was faulty. One of the main reasons could be that they just didn’t take into account that sometimes things happen randomly and we just don’t know why.

It’s good science to let people know if your study results are solid, or if they could have happened by chance. The usual way of doing this is to test your results with a p-value . A p value is a number that you get by running a hypothesis test on your data. A P value of 0.05 (5%) or less is usually enough to claim that your results are repeatable. However, there’s another way to test the validity of your results: Bayesian Hypothesis testing. This type of testing gives you another way to test the strength of your results.

Traditional testing (the type you probably came across in elementary stats or AP stats) is called Non-Bayesian. It is how often an outcome happens over repeated runs of the experiment. It’s an objective view of whether an experiment is repeatable. Bayesian hypothesis testing is a subjective view of the same thing. It takes into account how much faith you have in your results. In other words, would you wager money on the outcome of your experiment?

Differences Between Traditional and Bayesian Hypothesis Testing.

Traditional testing (Non Bayesian) requires you to repeat sampling over and over, while Bayesian testing does not. The main different between the two is in the first step of testing: stating a probability model. In Bayesian testing you add prior knowledge to this step. It also requires use of a posterior probability , which is the conditional probability given to a random event after all the evidence is considered.

Arguments for Bayesian Testing.

Many researchers think that it is a better alternative to traditional testing, because it:

• Includes prior knowledge about the data.
• Takes into account personal beliefs about the results.

Arguments against.

• Including prior data or knowledge isn’t justifiable.
• It is difficult to calculate compared to non-Bayesian testing.

Back to top

Hypothesis Testing Articles

• What is Ad Hoc Testing?
• Composite Hypothesis Test
• What is a Rejection Region?
• What is a Two Tailed Test?
• How to Decide if a Hypothesis Test is a One Tailed Test or a Two Tailed Test.
• How to Decide if a Hypothesis is a Left Tailed Test or a Right-Tailed Test.
• How to State the Null Hypothesis in Statistics.
• How to Find a Critical Value .
• How to Support or Reject a Null Hypothesis.

Specific Tests:

• Brunner Munzel Test (Generalized Wilcoxon Test).
• Chi Square Test for Normality.
• Cochran-Mantel-Haenszel Test.
• Granger Causality Test .
• Hotelling’s T-Squared.
• KPSS Test .
• What is a Likelihood-Ratio Test?
• Log rank test .
• MANCOVA Assumptions.
• MANCOVA Sample Size.
• Marascuilo Procedure
• Rao’s Spacing Test
• Rayleigh test of uniformity.
• Sequential Probability Ratio Test.
• How to Run a Sign Test.
• T Test: one sample.
• T-Test: Two sample .
• Welch’s ANOVA .
• Welch’s Test for Unequal Variances .
• Z-Test: one sample .
• Z Test: Two Proportion.
• Wald Test .

Related Articles:

• What is an Acceptance Region?
• How to Calculate Chebyshev’s Theorem.
• Contrast Analysis
• Decision Rule.
• Degrees of Freedom .
• Directional Test
• False Discovery Rate
• How to calculate the Least Significant Difference.
• Levels in Statistics.
• How to Calculate Margin of Error.
• Mean Difference (Difference in Means)
• The Multiple Testing Problem .
• What is the Neyman-Pearson Lemma?
• What is an Omnibus Test?
• One Sample Median Test .
• How to Find a Sample Size (General Instructions).
• Sig 2(Tailed) meaning in results
• What is a Standardized Test Statistic?
• How to Find Standard Error
• Standardized values: Example.
• How to Calculate a T-Score.
• T-Score Vs. a Z.Score.
• Testing a Single Mean.
• Unequal Sample Sizes.
• Uniformly Most Powerful Tests.
• How to Calculate a Z-Score.

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Hypothesis testing.

Key Topics:

• Basic approach
• Null and alternative hypothesis
• Decision making and the p -value
• Z-test & Nonparametric alternative

Basic approach to hypothesis testing

• State a model describing the relationship between the explanatory variables and the outcome variable(s) in the population and the nature of the variability. State all of your assumptions .
• Specify the null and alternative hypotheses in terms of the parameters of the model.
• Invent a test statistic that will tend to be different under the null and alternative hypotheses.
• Using the assumptions of step 1, find the theoretical sampling distribution of the statistic under the null hypothesis of step 2. Ideally the form of the sampling distribution should be one of the “standard distributions”(e.g. normal, t , binomial..)
• Calculate a p -value , as the area under the sampling distribution more extreme than your statistic. Depends on the form of the alternative hypothesis.
• Choose your acceptable type 1 error rate (alpha) and apply the decision rule : reject the null hypothesis if the p-value is less than alpha, otherwise do not reject.

• \(\frac{\bar{X}-\mu_0}{\sigma / \sqrt{n}}\)
• general form is: (estimate - value we are testing)/(st.dev of the estimate)
• z-statistic follows N(0,1) distribution
• 2 × the area above |z|, area above z,or area below z, or
• compare the statistic to a critical value, |z| ≥ z α/2 , z ≥ z α , or z ≤ - z α
• Choose the acceptable level of Alpha = 0.05, we conclude …. ?

Making the Decision

It is either likely or unlikely that we would collect the evidence we did given the initial assumption. (Note: “likely” or “unlikely” is measured by calculating a probability!)

If it is likely , then we “ do not reject ” our initial assumption. There is not enough evidence to do otherwise.

If it is unlikely , then:

• either our initial assumption is correct and we experienced an unusual event or,
• our initial assumption is incorrect

In statistics, if it is unlikely, we decide to “ reject ” our initial assumption.

Example: Criminal Trial Analogy

First, state 2 hypotheses, the null hypothesis (“H 0 ”) and the alternative hypothesis (“H A ”)

• H 0 : Defendant is not guilty.
• H A : Defendant is guilty.

Usually the H 0 is a statement of “no effect”, or “no change”, or “chance only” about a population parameter.

While the H A , depending on the situation, is that there is a difference, trend, effect, or a relationship with respect to a population parameter.

• It can one-sided and two-sided.
• In two-sided we only care there is a difference, but not the direction of it. In one-sided we care about a particular direction of the relationship. We want to know if the value is strictly larger or smaller.

Then, collect evidence, such as finger prints, blood spots, hair samples, carpet fibers, shoe prints, ransom notes, handwriting samples, etc. (In statistics, the data are the evidence.)

Next, you make your initial assumption.

• Defendant is innocent until proven guilty.

In statistics, we always assume the null hypothesis is true .

Then, make a decision based on the available evidence.

• If there is sufficient evidence (“beyond a reasonable doubt”), reject the null hypothesis . (Behave as if defendant is guilty.)
• If there is not enough evidence, do not reject the null hypothesis . (Behave as if defendant is not guilty.)

If the observed outcome, e.g., a sample statistic, is surprising under the assumption that the null hypothesis is true, but more probable if the alternative is true, then this outcome is evidence against H 0 and in favor of H A .

An observed effect so large that it would rarely occur by chance is called statistically significant (i.e., not likely to happen by chance).

Using the p -value to make the decision

The p -value represents how likely we would be to observe such an extreme sample if the null hypothesis were true. The p -value is a probability computed assuming the null hypothesis is true, that the test statistic would take a value as extreme or more extreme than that actually observed. Since it's a probability, it is a number between 0 and 1. The closer the number is to 0 means the event is “unlikely.” So if p -value is “small,” (typically, less than 0.05), we can then reject the null hypothesis.

Significance level and p -value

Significance level, α, is a decisive value for p -value. In this context, significant does not mean “important”, but it means “not likely to happened just by chance”.

α is the maximum probability of rejecting the null hypothesis when the null hypothesis is true. If α = 1 we always reject the null, if α = 0 we never reject the null hypothesis. In articles, journals, etc… you may read: “The results were significant ( p <0.05).” So if p =0.03, it's significant at the level of α = 0.05 but not at the level of α = 0.01. If we reject the H 0 at the level of α = 0.05 (which corresponds to 95% CI), we are saying that if H 0 is true, the observed phenomenon would happen no more than 5% of the time (that is 1 in 20). If we choose to compare the p -value to α = 0.01, we are insisting on a stronger evidence!

So, what kind of error could we make? No matter what decision we make, there is always a chance we made an error.

Errors in Criminal Trial:

Errors in Hypothesis Testing

Type I error (False positive): The null hypothesis is rejected when it is true.

• α is the maximum probability of making a Type I error.

Type II error (False negative): The null hypothesis is not rejected when it is false.

• β is the probability of making a Type II error

There is always a chance of making one of these errors. But, a good scientific study will minimize the chance of doing so!

The power of a statistical test is its probability of rejecting the null hypothesis if the null hypothesis is false. That is, power is the ability to correctly reject H 0 and detect a significant effect. In other words, power is one minus the type II error risk.

\(\text{Power }=1-\beta = P\left(\text{reject} H_0 | H_0 \text{is false } \right)\)

Which error is worse?

Type I = you are innocent, yet accused of cheating on the test. Type II = you cheated on the test, but you are found innocent.

This depends on the context of the problem too. But in most cases scientists are trying to be “conservative”; it's worse to make a spurious discovery than to fail to make a good one. Our goal it to increase the power of the test that is to minimize the length of the CI.

We need to keep in mind:

• the effect of the sample size,
• the correctness of the underlying assumptions about the population,
• statistical vs. practical significance, etc…

(see the handout). To study the tradeoffs between the sample size, α, and Type II error we can use power and operating characteristic curves.

What type of error might we have made?

Type I error is claiming that average student height is not 65 inches, when it really is. Type II error is failing to claim that the average student height is not 65in when it is.

We rejected the null hypothesis, i.e., claimed that the height is not 65, thus making potentially a Type I error. But sometimes the p -value is too low because of the large sample size, and we may have statistical significance but not really practical significance! That's why most statisticians are much more comfortable with using CI than tests.

There is a need for a further generalization. What if we can't assume that σ is known? In this case we would use s (the sample standard deviation) to estimate σ.

If the sample is very large, we can treat σ as known by assuming that σ = s . According to the law of large numbers, this is not too bad a thing to do. But if the sample is small, the fact that we have to estimate both the standard deviation and the mean adds extra uncertainty to our inference. In practice this means that we need a larger multiplier for the standard error.

We need one-sample t -test.

One sample t -test

• Assume data are independently sampled from a normal distribution with unknown mean μ and variance σ 2 . Make an initial assumption, μ 0 .

• t-statistic: \(\frac{\bar{X}-\mu_0}{s / \sqrt{n}}\) where s is a sample st.dev.
• t-statistic follows t -distribution with df = n - 1
• Alpha = 0.05, we conclude ….

Testing for the population proportion

Let's go back to our CNN poll. Assume we have a SRS of 1,017 adults.

We are interested in testing the following hypothesis: H 0 : p = 0.50 vs. p > 0.50

What is the test statistic?

If alpha = 0.05, what do we conclude?

We will see more details in the next lesson on proportions, then distributions, and possible tests.

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Lesson 10 of 24 By Avijeet Biswal

Table of Contents

In today’s data-driven world, decisions are based on data all the time. Hypothesis plays a crucial role in that process, whether it may be making business decisions, in the health sector, academia, or in quality improvement. Without hypothesis & hypothesis tests, you risk drawing the wrong conclusions and making bad decisions. In this tutorial, you will look at Hypothesis Testing in Statistics.

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What Is Hypothesis Testing in Statistics?

Hypothesis Testing is a type of statistical analysis in which you put your assumptions about a population parameter to the test. It is used to estimate the relationship between 2 statistical variables.

Let's discuss few examples of statistical hypothesis from real-life - 

• A teacher assumes that 60% of his college's students come from lower-middle-class families.
• A doctor believes that 3D (Diet, Dose, and Discipline) is 90% effective for diabetic patients.

Now that you know about hypothesis testing, look at the two types of hypothesis testing in statistics.

Hypothesis Testing Formula

Z = ( x̅ – μ0 ) / (σ /√n)

• Here, x̅ is the sample mean,
• μ0 is the population mean,
• σ is the standard deviation,
• n is the sample size.

How Hypothesis Testing Works?

An analyst performs hypothesis testing on a statistical sample to present evidence of the plausibility of the null hypothesis. Measurements and analyses are conducted on a random sample of the population to test a theory. Analysts use a random population sample to test two hypotheses: the null and alternative hypotheses.

The null hypothesis is typically an equality hypothesis between population parameters; for example, a null hypothesis may claim that the population means return equals zero. The alternate hypothesis is essentially the inverse of the null hypothesis (e.g., the population means the return is not equal to zero). As a result, they are mutually exclusive, and only one can be correct. One of the two possibilities, however, will always be correct.

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Null Hypothesis and Alternative Hypothesis

The Null Hypothesis is the assumption that the event will not occur. A null hypothesis has no bearing on the study's outcome unless it is rejected.

H0 is the symbol for it, and it is pronounced H-naught.

The Alternate Hypothesis is the logical opposite of the null hypothesis. The acceptance of the alternative hypothesis follows the rejection of the null hypothesis. H1 is the symbol for it.

Let's understand this with an example.

A sanitizer manufacturer claims that its product kills 95 percent of germs on average. 

To put this company's claim to the test, create a null and alternate hypothesis.

H0 (Null Hypothesis): Average = 95%.

Alternative Hypothesis (H1): The average is less than 95%.

Another straightforward example to understand this concept is determining whether or not a coin is fair and balanced. The null hypothesis states that the probability of a show of heads is equal to the likelihood of a show of tails. In contrast, the alternate theory states that the probability of a show of heads and tails would be very different.

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Hypothesis Testing Calculation With Examples

Let's consider a hypothesis test for the average height of women in the United States. Suppose our null hypothesis is that the average height is 5'4". We gather a sample of 100 women and determine that their average height is 5'5". The standard deviation of population is 2.

To calculate the z-score, we would use the following formula:

z = ( x̅ – μ0 ) / (σ /√n)

z = (5'5" - 5'4") / (2" / √100)

z = 0.5 / (0.045)

We will reject the null hypothesis as the z-score of 11.11 is very large and conclude that there is evidence to suggest that the average height of women in the US is greater than 5'4".

Steps in Hypothesis Testing

Hypothesis testing is a statistical method to determine if there is enough evidence in a sample of data to infer that a certain condition is true for the entire population. Here’s a breakdown of the typical steps involved in hypothesis testing:

Formulate Hypotheses

• Null Hypothesis (H0): This hypothesis states that there is no effect or difference, and it is the hypothesis you attempt to reject with your test.
• Alternative Hypothesis (H1 or Ha): This hypothesis is what you might believe to be true or hope to prove true. It is usually considered the opposite of the null hypothesis.

Choose the Significance Level (α)

The significance level, often denoted by alpha (α), is the probability of rejecting the null hypothesis when it is true. Common choices for α are 0.05 (5%), 0.01 (1%), and 0.10 (10%).

Select the Appropriate Test

Choose a statistical test based on the type of data and the hypothesis. Common tests include t-tests, chi-square tests, ANOVA, and regression analysis. The selection depends on data type, distribution, sample size, and whether the hypothesis is one-tailed or two-tailed.

Collect Data

Gather the data that will be analyzed in the test. This data should be representative of the population to infer conclusions accurately.

Calculate the Test Statistic

Based on the collected data and the chosen test, calculate a test statistic that reflects how much the observed data deviates from the null hypothesis.

Determine the p-value

The p-value is the probability of observing test results at least as extreme as the results observed, assuming the null hypothesis is correct. It helps determine the strength of the evidence against the null hypothesis.

Make a Decision

Compare the p-value to the chosen significance level:

• If the p-value ≤ α: Reject the null hypothesis, suggesting sufficient evidence in the data supports the alternative hypothesis.
• If the p-value > α: Do not reject the null hypothesis, suggesting insufficient evidence to support the alternative hypothesis.

Report the Results

Present the findings from the hypothesis test, including the test statistic, p-value, and the conclusion about the hypotheses.

Perform Post-hoc Analysis (if necessary)

Depending on the results and the study design, further analysis may be needed to explore the data more deeply or to address multiple comparisons if several hypotheses were tested simultaneously.

Types of Hypothesis Testing

To determine whether a discovery or relationship is statistically significant, hypothesis testing uses a z-test. It usually checks to see if two means are the same (the null hypothesis). Only when the population standard deviation is known and the sample size is 30 data points or more, can a z-test be applied.

A statistical test called a t-test is employed to compare the means of two groups. To determine whether two groups differ or if a procedure or treatment affects the population of interest, it is frequently used in hypothesis testing.

Chi-Square 

You utilize a Chi-square test for hypothesis testing concerning whether your data is as predicted. To determine if the expected and observed results are well-fitted, the Chi-square test analyzes the differences between categorical variables from a random sample. The test's fundamental premise is that the observed values in your data should be compared to the predicted values that would be present if the null hypothesis were true.

Hypothesis Testing and Confidence Intervals

Both confidence intervals and hypothesis tests are inferential techniques that depend on approximating the sample distribution. Data from a sample is used to estimate a population parameter using confidence intervals. Data from a sample is used in hypothesis testing to examine a given hypothesis. We must have a postulated parameter to conduct hypothesis testing.

Bootstrap distributions and randomization distributions are created using comparable simulation techniques. The observed sample statistic is the focal point of a bootstrap distribution, whereas the null hypothesis value is the focal point of a randomization distribution.

A variety of feasible population parameter estimates are included in confidence ranges. In this lesson, we created just two-tailed confidence intervals. There is a direct connection between these two-tail confidence intervals and these two-tail hypothesis tests. The results of a two-tailed hypothesis test and two-tailed confidence intervals typically provide the same results. In other words, a hypothesis test at the 0.05 level will virtually always fail to reject the null hypothesis if the 95% confidence interval contains the predicted value. A hypothesis test at the 0.05 level will nearly certainly reject the null hypothesis if the 95% confidence interval does not include the hypothesized parameter.

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Simple and Composite Hypothesis Testing

Depending on the population distribution, you can classify the statistical hypothesis into two types.

Simple Hypothesis: A simple hypothesis specifies an exact value for the parameter.

Composite Hypothesis: A composite hypothesis specifies a range of values.

A company is claiming that their average sales for this quarter are 1000 units. This is an example of a simple hypothesis.

Suppose the company claims that the sales are in the range of 900 to 1000 units. Then this is a case of a composite hypothesis.

One-Tailed and Two-Tailed Hypothesis Testing

The One-Tailed test, also called a directional test, considers a critical region of data that would result in the null hypothesis being rejected if the test sample falls into it, inevitably meaning the acceptance of the alternate hypothesis.

In a one-tailed test, the critical distribution area is one-sided, meaning the test sample is either greater or lesser than a specific value.

In two tails, the test sample is checked to be greater or less than a range of values in a Two-Tailed test, implying that the critical distribution area is two-sided.

If the sample falls within this range, the alternate hypothesis will be accepted, and the null hypothesis will be rejected.

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Right Tailed Hypothesis Testing

If the larger than (>) sign appears in your hypothesis statement, you are using a right-tailed test, also known as an upper test. Or, to put it another way, the disparity is to the right. For instance, you can contrast the battery life before and after a change in production. Your hypothesis statements can be the following if you want to know if the battery life is longer than the original (let's say 90 hours):

• The null hypothesis is (H0 <= 90) or less change.
• A possibility is that battery life has risen (H1) > 90.

The crucial point in this situation is that the alternate hypothesis (H1), not the null hypothesis, decides whether you get a right-tailed test.

Left Tailed Hypothesis Testing

Alternative hypotheses that assert the true value of a parameter is lower than the null hypothesis are tested with a left-tailed test; they are indicated by the asterisk "<".

Suppose H0: mean = 50 and H1: mean not equal to 50

According to the H1, the mean can be greater than or less than 50. This is an example of a Two-tailed test.

In a similar manner, if H0: mean >=50, then H1: mean <50

Here the mean is less than 50. It is called a One-tailed test.

Type 1 and Type 2 Error

A hypothesis test can result in two types of errors.

Type 1 Error: A Type-I error occurs when sample results reject the null hypothesis despite being true.

Type 2 Error: A Type-II error occurs when the null hypothesis is not rejected when it is false, unlike a Type-I error.

Suppose a teacher evaluates the examination paper to decide whether a student passes or fails.

H0: Student has passed

H1: Student has failed

Type I error will be the teacher failing the student [rejects H0] although the student scored the passing marks [H0 was true]. 

Type II error will be the case where the teacher passes the student [do not reject H0] although the student did not score the passing marks [H1 is true].

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Limitations of Hypothesis Testing

Hypothesis testing has some limitations that researchers should be aware of:

• It cannot prove or establish the truth: Hypothesis testing provides evidence to support or reject a hypothesis, but it cannot confirm the absolute truth of the research question.
• Results are sample-specific: Hypothesis testing is based on analyzing a sample from a population, and the conclusions drawn are specific to that particular sample.
• Possible errors: During hypothesis testing, there is a chance of committing type I error (rejecting a true null hypothesis) or type II error (failing to reject a false null hypothesis).
• Assumptions and requirements: Different tests have specific assumptions and requirements that must be met to accurately interpret results.

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After reading this tutorial, you would have a much better understanding of hypothesis testing, one of the most important concepts in the field of Data Science . The majority of hypotheses are based on speculation about observed behavior, natural phenomena, or established theories.

If you are interested in statistics of data science and skills needed for such a career, you ought to explore the Post Graduate Program in Data Science.

If you have any questions regarding this ‘Hypothesis Testing In Statistics’ tutorial, do share them in the comment section. Our subject matter expert will respond to your queries. Happy learning!

1. What is hypothesis testing in statistics with example?

Hypothesis testing is a statistical method used to determine if there is enough evidence in a sample data to draw conclusions about a population. It involves formulating two competing hypotheses, the null hypothesis (H0) and the alternative hypothesis (Ha), and then collecting data to assess the evidence. An example: testing if a new drug improves patient recovery (Ha) compared to the standard treatment (H0) based on collected patient data.

2. What is H0 and H1 in statistics?

In statistics, H0​ and H1​ represent the null and alternative hypotheses. The null hypothesis, H0​, is the default assumption that no effect or difference exists between groups or conditions. The alternative hypothesis, H1​, is the competing claim suggesting an effect or a difference. Statistical tests determine whether to reject the null hypothesis in favor of the alternative hypothesis based on the data.

3. What is a simple hypothesis with an example?

A simple hypothesis is a specific statement predicting a single relationship between two variables. It posits a direct and uncomplicated outcome. For example, a simple hypothesis might state, "Increased sunlight exposure increases the growth rate of sunflowers." Here, the hypothesis suggests a direct relationship between the amount of sunlight (independent variable) and the growth rate of sunflowers (dependent variable), with no additional variables considered.

4. What are the 3 major types of hypothesis?

The three major types of hypotheses are:

• Null Hypothesis (H0): Represents the default assumption, stating that there is no significant effect or relationship in the data.
• Alternative Hypothesis (Ha): Contradicts the null hypothesis and proposes a specific effect or relationship that researchers want to investigate.
• Nondirectional Hypothesis: An alternative hypothesis that doesn't specify the direction of the effect, leaving it open for both positive and negative possibilities.

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Hypothesis Testing

Hypothesis testing is a tool for making statistical inferences about the population data. It is an analysis tool that tests assumptions and determines how likely something is within a given standard of accuracy. Hypothesis testing provides a way to verify whether the results of an experiment are valid.

A null hypothesis and an alternative hypothesis are set up before performing the hypothesis testing. This helps to arrive at a conclusion regarding the sample obtained from the population. In this article, we will learn more about hypothesis testing, its types, steps to perform the testing, and associated examples.

What is Hypothesis Testing in Statistics?

Hypothesis testing uses sample data from the population to draw useful conclusions regarding the population probability distribution . It tests an assumption made about the data using different types of hypothesis testing methodologies. The hypothesis testing results in either rejecting or not rejecting the null hypothesis.

Hypothesis Testing Definition

Hypothesis testing can be defined as a statistical tool that is used to identify if the results of an experiment are meaningful or not. It involves setting up a null hypothesis and an alternative hypothesis. These two hypotheses will always be mutually exclusive. This means that if the null hypothesis is true then the alternative hypothesis is false and vice versa. An example of hypothesis testing is setting up a test to check if a new medicine works on a disease in a more efficient manner.

Null Hypothesis

The null hypothesis is a concise mathematical statement that is used to indicate that there is no difference between two possibilities. In other words, there is no difference between certain characteristics of data. This hypothesis assumes that the outcomes of an experiment are based on chance alone. It is denoted as \(H_{0}\). Hypothesis testing is used to conclude if the null hypothesis can be rejected or not. Suppose an experiment is conducted to check if girls are shorter than boys at the age of 5. The null hypothesis will say that they are the same height.

Alternative Hypothesis

The alternative hypothesis is an alternative to the null hypothesis. It is used to show that the observations of an experiment are due to some real effect. It indicates that there is a statistical significance between two possible outcomes and can be denoted as \(H_{1}\) or \(H_{a}\). For the above-mentioned example, the alternative hypothesis would be that girls are shorter than boys at the age of 5.

Hypothesis Testing P Value

In hypothesis testing, the p value is used to indicate whether the results obtained after conducting a test are statistically significant or not. It also indicates the probability of making an error in rejecting or not rejecting the null hypothesis.This value is always a number between 0 and 1. The p value is compared to an alpha level, \(\alpha\) or significance level. The alpha level can be defined as the acceptable risk of incorrectly rejecting the null hypothesis. The alpha level is usually chosen between 1% to 5%.

Hypothesis Testing Critical region

All sets of values that lead to rejecting the null hypothesis lie in the critical region. Furthermore, the value that separates the critical region from the non-critical region is known as the critical value.

Hypothesis Testing Formula

Depending upon the type of data available and the size, different types of hypothesis testing are used to determine whether the null hypothesis can be rejected or not. The hypothesis testing formula for some important test statistics are given below:

• z = \(\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}\). \(\overline{x}\) is the sample mean, \(\mu\) is the population mean, \(\sigma\) is the population standard deviation and n is the size of the sample.
• t = \(\frac{\overline{x}-\mu}{\frac{s}{\sqrt{n}}}\). s is the sample standard deviation.
• \(\chi ^{2} = \sum \frac{(O_{i}-E_{i})^{2}}{E_{i}}\). \(O_{i}\) is the observed value and \(E_{i}\) is the expected value.

We will learn more about these test statistics in the upcoming section.

Types of Hypothesis Testing

Selecting the correct test for performing hypothesis testing can be confusing. These tests are used to determine a test statistic on the basis of which the null hypothesis can either be rejected or not rejected. Some of the important tests used for hypothesis testing are given below.

Hypothesis Testing Z Test

A z test is a way of hypothesis testing that is used for a large sample size (n ≥ 30). It is used to determine whether there is a difference between the population mean and the sample mean when the population standard deviation is known. It can also be used to compare the mean of two samples. It is used to compute the z test statistic. The formulas are given as follows:

• One sample: z = \(\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}\).
• Two samples: z = \(\frac{(\overline{x_{1}}-\overline{x_{2}})-(\mu_{1}-\mu_{2})}{\sqrt{\frac{\sigma_{1}^{2}}{n_{1}}+\frac{\sigma_{2}^{2}}{n_{2}}}}\).

Hypothesis Testing t Test

The t test is another method of hypothesis testing that is used for a small sample size (n < 30). It is also used to compare the sample mean and population mean. However, the population standard deviation is not known. Instead, the sample standard deviation is known. The mean of two samples can also be compared using the t test.

• One sample: t = \(\frac{\overline{x}-\mu}{\frac{s}{\sqrt{n}}}\).
• Two samples: t = \(\frac{(\overline{x_{1}}-\overline{x_{2}})-(\mu_{1}-\mu_{2})}{\sqrt{\frac{s_{1}^{2}}{n_{1}}+\frac{s_{2}^{2}}{n_{2}}}}\).

Hypothesis Testing Chi Square

The Chi square test is a hypothesis testing method that is used to check whether the variables in a population are independent or not. It is used when the test statistic is chi-squared distributed.

One Tailed Hypothesis Testing

One tailed hypothesis testing is done when the rejection region is only in one direction. It can also be known as directional hypothesis testing because the effects can be tested in one direction only. This type of testing is further classified into the right tailed test and left tailed test.

Right Tailed Hypothesis Testing

The right tail test is also known as the upper tail test. This test is used to check whether the population parameter is greater than some value. The null and alternative hypotheses for this test are given as follows:

\(H_{0}\): The population parameter is ≤ some value

\(H_{1}\): The population parameter is > some value.

If the test statistic has a greater value than the critical value then the null hypothesis is rejected

Left Tailed Hypothesis Testing

The left tail test is also known as the lower tail test. It is used to check whether the population parameter is less than some value. The hypotheses for this hypothesis testing can be written as follows:

\(H_{0}\): The population parameter is ≥ some value

\(H_{1}\): The population parameter is < some value.

The null hypothesis is rejected if the test statistic has a value lesser than the critical value.

Two Tailed Hypothesis Testing

In this hypothesis testing method, the critical region lies on both sides of the sampling distribution. It is also known as a non - directional hypothesis testing method. The two-tailed test is used when it needs to be determined if the population parameter is assumed to be different than some value. The hypotheses can be set up as follows:

\(H_{0}\): the population parameter = some value

\(H_{1}\): the population parameter ≠ some value

The null hypothesis is rejected if the test statistic has a value that is not equal to the critical value.

Hypothesis Testing Steps

Hypothesis testing can be easily performed in five simple steps. The most important step is to correctly set up the hypotheses and identify the right method for hypothesis testing. The basic steps to perform hypothesis testing are as follows:

• Step 1: Set up the null hypothesis by correctly identifying whether it is the left-tailed, right-tailed, or two-tailed hypothesis testing.
• Step 2: Set up the alternative hypothesis.
• Step 3: Choose the correct significance level, \(\alpha\), and find the critical value.
• Step 4: Calculate the correct test statistic (z, t or \(\chi\)) and p-value.
• Step 5: Compare the test statistic with the critical value or compare the p-value with \(\alpha\) to arrive at a conclusion. In other words, decide if the null hypothesis is to be rejected or not.

Hypothesis Testing Example

The best way to solve a problem on hypothesis testing is by applying the 5 steps mentioned in the previous section. Suppose a researcher claims that the mean average weight of men is greater than 100kgs with a standard deviation of 15kgs. 30 men are chosen with an average weight of 112.5 Kgs. Using hypothesis testing, check if there is enough evidence to support the researcher's claim. The confidence interval is given as 95%.

Step 1: This is an example of a right-tailed test. Set up the null hypothesis as \(H_{0}\): \(\mu\) = 100.

Step 2: The alternative hypothesis is given by \(H_{1}\): \(\mu\) > 100.

Step 3: As this is a one-tailed test, \(\alpha\) = 100% - 95% = 5%. This can be used to determine the critical value.

1 - \(\alpha\) = 1 - 0.05 = 0.95

0.95 gives the required area under the curve. Now using a normal distribution table, the area 0.95 is at z = 1.645. A similar process can be followed for a t-test. The only additional requirement is to calculate the degrees of freedom given by n - 1.

Step 4: Calculate the z test statistic. This is because the sample size is 30. Furthermore, the sample and population means are known along with the standard deviation.

z = \(\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}\).

\(\mu\) = 100, \(\overline{x}\) = 112.5, n = 30, \(\sigma\) = 15

z = \(\frac{112.5-100}{\frac{15}{\sqrt{30}}}\) = 4.56

Step 5: Conclusion. As 4.56 > 1.645 thus, the null hypothesis can be rejected.

Hypothesis Testing and Confidence Intervals

Confidence intervals form an important part of hypothesis testing. This is because the alpha level can be determined from a given confidence interval. Suppose a confidence interval is given as 95%. Subtract the confidence interval from 100%. This gives 100 - 95 = 5% or 0.05. This is the alpha value of a one-tailed hypothesis testing. To obtain the alpha value for a two-tailed hypothesis testing, divide this value by 2. This gives 0.05 / 2 = 0.025.

Related Articles:

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• Data Handling

Important Notes on Hypothesis Testing

• Hypothesis testing is a technique that is used to verify whether the results of an experiment are statistically significant.
• It involves the setting up of a null hypothesis and an alternate hypothesis.
• There are three types of tests that can be conducted under hypothesis testing - z test, t test, and chi square test.
• Hypothesis testing can be classified as right tail, left tail, and two tail tests.

Examples on Hypothesis Testing

• Example 1: The average weight of a dumbbell in a gym is 90lbs. However, a physical trainer believes that the average weight might be higher. A random sample of 5 dumbbells with an average weight of 110lbs and a standard deviation of 18lbs. Using hypothesis testing check if the physical trainer's claim can be supported for a 95% confidence level. Solution: As the sample size is lesser than 30, the t-test is used. \(H_{0}\): \(\mu\) = 90, \(H_{1}\): \(\mu\) > 90 \(\overline{x}\) = 110, \(\mu\) = 90, n = 5, s = 18. \(\alpha\) = 0.05 Using the t-distribution table, the critical value is 2.132 t = \(\frac{\overline{x}-\mu}{\frac{s}{\sqrt{n}}}\) t = 2.484 As 2.484 > 2.132, the null hypothesis is rejected. Answer: The average weight of the dumbbells may be greater than 90lbs
• Example 2: The average score on a test is 80 with a standard deviation of 10. With a new teaching curriculum introduced it is believed that this score will change. On random testing, the score of 38 students, the mean was found to be 88. With a 0.05 significance level, is there any evidence to support this claim? Solution: This is an example of two-tail hypothesis testing. The z test will be used. \(H_{0}\): \(\mu\) = 80, \(H_{1}\): \(\mu\) ≠ 80 \(\overline{x}\) = 88, \(\mu\) = 80, n = 36, \(\sigma\) = 10. \(\alpha\) = 0.05 / 2 = 0.025 The critical value using the normal distribution table is 1.96 z = \(\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}\) z = \(\frac{88-80}{\frac{10}{\sqrt{36}}}\) = 4.8 As 4.8 > 1.96, the null hypothesis is rejected. Answer: There is a difference in the scores after the new curriculum was introduced.
• Example 3: The average score of a class is 90. However, a teacher believes that the average score might be lower. The scores of 6 students were randomly measured. The mean was 82 with a standard deviation of 18. With a 0.05 significance level use hypothesis testing to check if this claim is true. Solution: The t test will be used. \(H_{0}\): \(\mu\) = 90, \(H_{1}\): \(\mu\) < 90 \(\overline{x}\) = 110, \(\mu\) = 90, n = 6, s = 18 The critical value from the t table is -2.015 t = \(\frac{\overline{x}-\mu}{\frac{s}{\sqrt{n}}}\) t = \(\frac{82-90}{\frac{18}{\sqrt{6}}}\) t = -1.088 As -1.088 > -2.015, we fail to reject the null hypothesis. Answer: There is not enough evidence to support the claim.

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FAQs on Hypothesis Testing

What is hypothesis testing.

Hypothesis testing in statistics is a tool that is used to make inferences about the population data. It is also used to check if the results of an experiment are valid.

What is the z Test in Hypothesis Testing?

The z test in hypothesis testing is used to find the z test statistic for normally distributed data . The z test is used when the standard deviation of the population is known and the sample size is greater than or equal to 30.

What is the t Test in Hypothesis Testing?

The t test in hypothesis testing is used when the data follows a student t distribution . It is used when the sample size is less than 30 and standard deviation of the population is not known.

What is the formula for z test in Hypothesis Testing?

The formula for a one sample z test in hypothesis testing is z = \(\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}\) and for two samples is z = \(\frac{(\overline{x_{1}}-\overline{x_{2}})-(\mu_{1}-\mu_{2})}{\sqrt{\frac{\sigma_{1}^{2}}{n_{1}}+\frac{\sigma_{2}^{2}}{n_{2}}}}\).

What is the p Value in Hypothesis Testing?

The p value helps to determine if the test results are statistically significant or not. In hypothesis testing, the null hypothesis can either be rejected or not rejected based on the comparison between the p value and the alpha level.

What is One Tail Hypothesis Testing?

When the rejection region is only on one side of the distribution curve then it is known as one tail hypothesis testing. The right tail test and the left tail test are two types of directional hypothesis testing.

What is the Alpha Level in Two Tail Hypothesis Testing?

To get the alpha level in a two tail hypothesis testing divide \(\alpha\) by 2. This is done as there are two rejection regions in the curve.

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• Definition/Introduction

Medical providers often rely on evidence-based medicine to guide decision-making in practice. Often a research hypothesis is tested with results provided, typically with p values, confidence intervals, or both. Additionally, statistical or research significance is estimated or determined by the investigators. Unfortunately, healthcare providers may have different comfort levels in interpreting these findings, which may affect the adequate application of the data.

• Issues of Concern

Without a foundational understanding of hypothesis testing, p values, confidence intervals, and the difference between statistical and clinical significance, it may affect healthcare providers' ability to make clinical decisions without relying purely on the research investigators deemed level of significance. Therefore, an overview of these concepts is provided to allow medical professionals to use their expertise to determine if results are reported sufficiently and if the study outcomes are clinically appropriate to be applied in healthcare practice.

Hypothesis Testing

Investigators conducting studies need research questions and hypotheses to guide analyses. Starting with broad research questions (RQs), investigators then identify a gap in current clinical practice or research. Any research problem or statement is grounded in a better understanding of relationships between two or more variables. For this article, we will use the following research question example:

Research Question: Is Drug 23 an effective treatment for Disease A?

Research questions do not directly imply specific guesses or predictions; we must formulate research hypotheses. A hypothesis is a predetermined declaration regarding the research question in which the investigator(s) makes a precise, educated guess about a study outcome. This is sometimes called the alternative hypothesis and ultimately allows the researcher to take a stance based on experience or insight from medical literature. An example of a hypothesis is below.

Research Hypothesis: Drug 23 will significantly reduce symptoms associated with Disease A compared to Drug 22.

The null hypothesis states that there is no statistical difference between groups based on the stated research hypothesis.

Researchers should be aware of journal recommendations when considering how to report p values, and manuscripts should remain internally consistent.

Regarding p values, as the number of individuals enrolled in a study (the sample size) increases, the likelihood of finding a statistically significant effect increases. With very large sample sizes, the p-value can be very low significant differences in the reduction of symptoms for Disease A between Drug 23 and Drug 22. The null hypothesis is deemed true until a study presents significant data to support rejecting the null hypothesis. Based on the results, the investigators will either reject the null hypothesis (if they found significant differences or associations) or fail to reject the null hypothesis (they could not provide proof that there were significant differences or associations).

To test a hypothesis, researchers obtain data on a representative sample to determine whether to reject or fail to reject a null hypothesis. In most research studies, it is not feasible to obtain data for an entire population. Using a sampling procedure allows for statistical inference, though this involves a certain possibility of error. [1]  When determining whether to reject or fail to reject the null hypothesis, mistakes can be made: Type I and Type II errors. Though it is impossible to ensure that these errors have not occurred, researchers should limit the possibilities of these faults. [2]

Significance

Significance is a term to describe the substantive importance of medical research. Statistical significance is the likelihood of results due to chance. [3]  Healthcare providers should always delineate statistical significance from clinical significance, a common error when reviewing biomedical research. [4]  When conceptualizing findings reported as either significant or not significant, healthcare providers should not simply accept researchers' results or conclusions without considering the clinical significance. Healthcare professionals should consider the clinical importance of findings and understand both p values and confidence intervals so they do not have to rely on the researchers to determine the level of significance. [5]  One criterion often used to determine statistical significance is the utilization of p values.

P values are used in research to determine whether the sample estimate is significantly different from a hypothesized value. The p-value is the probability that the observed effect within the study would have occurred by chance if, in reality, there was no true effect. Conventionally, data yielding a p<0.05 or p<0.01 is considered statistically significant. While some have debated that the 0.05 level should be lowered, it is still universally practiced. [6]  Hypothesis testing allows us to determine the size of the effect.

An example of findings reported with p values are below:

Statement: Drug 23 reduced patients' symptoms compared to Drug 22. Patients who received Drug 23 (n=100) were 2.1 times less likely than patients who received Drug 22 (n = 100) to experience symptoms of Disease A, p<0.05.

Statement:Individuals who were prescribed Drug 23 experienced fewer symptoms (M = 1.3, SD = 0.7) compared to individuals who were prescribed Drug 22 (M = 5.3, SD = 1.9). This finding was statistically significant, p= 0.02.

For either statement, if the threshold had been set at 0.05, the null hypothesis (that there was no relationship) should be rejected, and we should conclude significant differences. Noticeably, as can be seen in the two statements above, some researchers will report findings with < or > and others will provide an exact p-value (0.000001) but never zero [6] . When examining research, readers should understand how p values are reported. The best practice is to report all p values for all variables within a study design, rather than only providing p values for variables with significant findings. [7]  The inclusion of all p values provides evidence for study validity and limits suspicion for selective reporting/data mining.  

While researchers have historically used p values, experts who find p values problematic encourage the use of confidence intervals. [8] . P-values alone do not allow us to understand the size or the extent of the differences or associations. [3]  In March 2016, the American Statistical Association (ASA) released a statement on p values, noting that scientific decision-making and conclusions should not be based on a fixed p-value threshold (e.g., 0.05). They recommend focusing on the significance of results in the context of study design, quality of measurements, and validity of data. Ultimately, the ASA statement noted that in isolation, a p-value does not provide strong evidence. [9]

When conceptualizing clinical work, healthcare professionals should consider p values with a concurrent appraisal study design validity. For example, a p-value from a double-blinded randomized clinical trial (designed to minimize bias) should be weighted higher than one from a retrospective observational study [7] . The p-value debate has smoldered since the 1950s [10] , and replacement with confidence intervals has been suggested since the 1980s. [11]

Confidence Intervals

A confidence interval provides a range of values within given confidence (e.g., 95%), including the accurate value of the statistical constraint within a targeted population. [12]  Most research uses a 95% CI, but investigators can set any level (e.g., 90% CI, 99% CI). [13]  A CI provides a range with the lower bound and upper bound limits of a difference or association that would be plausible for a population. [14]  Therefore, a CI of 95% indicates that if a study were to be carried out 100 times, the range would contain the true value in 95, [15]  confidence intervals provide more evidence regarding the precision of an estimate compared to p-values. [6]

In consideration of the similar research example provided above, one could make the following statement with 95% CI:

Statement: Individuals who were prescribed Drug 23 had no symptoms after three days, which was significantly faster than those prescribed Drug 22; there was a mean difference between the two groups of days to the recovery of 4.2 days (95% CI: 1.9 – 7.8).

It is important to note that the width of the CI is affected by the standard error and the sample size; reducing a study sample number will result in less precision of the CI (increase the width). [14]  A larger width indicates a smaller sample size or a larger variability. [16]  A researcher would want to increase the precision of the CI. For example, a 95% CI of 1.43 – 1.47 is much more precise than the one provided in the example above. In research and clinical practice, CIs provide valuable information on whether the interval includes or excludes any clinically significant values. [14]

Null values are sometimes used for differences with CI (zero for differential comparisons and 1 for ratios). However, CIs provide more information than that. [15]  Consider this example: A hospital implements a new protocol that reduced wait time for patients in the emergency department by an average of 25 minutes (95% CI: -2.5 – 41 minutes). Because the range crosses zero, implementing this protocol in different populations could result in longer wait times; however, the range is much higher on the positive side. Thus, while the p-value used to detect statistical significance for this may result in "not significant" findings, individuals should examine this range, consider the study design, and weigh whether or not it is still worth piloting in their workplace.

Similarly to p-values, 95% CIs cannot control for researchers' errors (e.g., study bias or improper data analysis). [14]  In consideration of whether to report p-values or CIs, researchers should examine journal preferences. When in doubt, reporting both may be beneficial. [13]  An example is below:

Reporting both: Individuals who were prescribed Drug 23 had no symptoms after three days, which was significantly faster than those prescribed Drug 22, p = 0.009. There was a mean difference between the two groups of days to the recovery of 4.2 days (95% CI: 1.9 – 7.8).

• Clinical Significance

Recall that clinical significance and statistical significance are two different concepts. Healthcare providers should remember that a study with statistically significant differences and large sample size may be of no interest to clinicians, whereas a study with smaller sample size and statistically non-significant results could impact clinical practice. [14]  Additionally, as previously mentioned, a non-significant finding may reflect the study design itself rather than relationships between variables.

Healthcare providers using evidence-based medicine to inform practice should use clinical judgment to determine the practical importance of studies through careful evaluation of the design, sample size, power, likelihood of type I and type II errors, data analysis, and reporting of statistical findings (p values, 95% CI or both). [4]  Interestingly, some experts have called for "statistically significant" or "not significant" to be excluded from work as statistical significance never has and will never be equivalent to clinical significance. [17]

The decision on what is clinically significant can be challenging, depending on the providers' experience and especially the severity of the disease. Providers should use their knowledge and experiences to determine the meaningfulness of study results and make inferences based not only on significant or insignificant results by researchers but through their understanding of study limitations and practical implications.

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All physicians, nurses, pharmacists, and other healthcare professionals should strive to understand the concepts in this chapter. These individuals should maintain the ability to review and incorporate new literature for evidence-based and safe care. 

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Disclosure: Jacob Shreffler declares no relevant financial relationships with ineligible companies.

Disclosure: Martin Huecker declares no relevant financial relationships with ineligible companies.

This book is distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) ( http://creativecommons.org/licenses/by-nc-nd/4.0/ ), which permits others to distribute the work, provided that the article is not altered or used commercially. You are not required to obtain permission to distribute this article, provided that you credit the author and journal.

• Cite this Page Shreffler J, Huecker MR. Hypothesis Testing, P Values, Confidence Intervals, and Significance. [Updated 2023 Mar 13]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2024 Jan-.

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Summary of the 3 Approaches to Hypothesis Testing

Steps to conduct a hypothesis test using $p$-values:.

Identify the null hypothesis and the alternative hypothesis (and decide which is the claim).

Ensure any necessary assumptions are met for the test to be conducted.

Find the test statistic.

Find the p-value associated with the test statistic as it relates to the alternative hypothesis.

Compare the p-value with the significance level, $\alpha$. If $p \lt \alpha$, conclude that the null hypothesis should be rejected based on what we saw. If not, conclude that we fail to reject the null hypothesis as a result of what we saw.

Make an inference.

Steps to Conduct a Hypothesis Test Using Critical Values:

Find the critical values associated with the significance level, $\alpha$, and the alternative hypothesis to establish the rejection region in the distribution.

If the test statistic falls in the rejection region, conclude that the null hypothesis should be rejected based on what we saw. If not, conclude that we fail to reject the null hypothesis as a result of what we saw.

Steps to Conduct a Hypothesis Test Using a Confidence Interval:

Construct a confidence interval with a confidence level of $(1-\alpha)$

If the hypothesized population parameter falls outside of the confidence interval, conclude that the null hypothesis should be rejected based on what we saw. If it falls within the confidence interval, conclude that we fail to reject the null hypothesis as a result of what we saw.

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Why hypothesis testing is important in research ?

Hypothesis Testing allows researchers to evaluate the validity of their assumptions and draw conclusions based on evidence. It provides a framework for making predictions and determining whether observed results are statistically significant or just occurred by chance. By applying various statistical tests, researchers can measure the strength of the evidence and quantify the uncertainty associated with their findings.

Table of Content

Importance of Hypothesis Testing in Research

Types of hypothesis tests, common errors in hypothesis testing, interpreting the results of hypothesis tests, examples of hypothesis testing in different fields, tools and software for conducting hypothesis tests.

Understanding the importance of hypothesis testing is essential for conducting rigorous and reliable research. It enables researchers to make well-informed decisions, support or challenge existing theories, and contribute to the advancement of knowledge in their respective fields. So, whether you are a scientist, a market analyst, or a student working on a research project, grasp the power of hypothesis testing and elevate the impact of your data analysis.

Hypothesis testing is the cornerstone of the scientific method and plays a vital role in the research process. It allows researchers to make informed decisions and draw reliable conclusions from their data. By formulating a hypothesis and then testing it against the observed data, researchers can determine whether their initial assumptions are supported or refuted. This systematic approach is crucial for advancing knowledge and understanding in various fields, from medicine and psychology to economics and engineering. Hypothesis testing enables researchers to move beyond mere observations and anecdotal evidence, and instead rely on statistical analysis to quantify the strength of their findings. It helps them differentiate between genuine effects and random fluctuations, ensuring that the conclusions drawn are based on rigorous and objective analysis.

Moreover, hypothesis testing is not limited to academic research; it is equally important in the business world, where data-driven decision-making is essential for success. Marketers, for instance, can use hypothesis testing to evaluate the effectiveness of their advertising campaigns, while financial analysts can use it to assess the performance of investment strategies. By incorporating hypothesis testing into their decision-making processes, organizations can make more informed choices and optimize their operations.

Understanding the Null and Alternative Hypotheses

At the heart of hypothesis testing lies the distinction between the null hypothesis (H0) and the alternative hypothesis (H1). The null hypothesis represents the status quo or the assumption that there is no significant difference or relationship between the variables being studied. Conversely, the alternative hypothesis suggests that there is a meaningful difference or relationship that is worth investigating.

Researchers begin by formulating their null and alternative hypotheses based on their research question and existing knowledge. For example, in a study examining the effect of a new drug on blood pressure, the null hypothesis might be that the drug has no effect on blood pressure, while the alternative hypothesis would be that the drug does have an effect on blood pressure.

Steps Involved in Hypothesis Testing

Hypothesis testing is a structured process that involves several key steps:

• Clearly define the research question and formulate the null and alternative hypotheses.
• Select an appropriate statistical test based on the nature of the data and research question.
• Collect and organize data, ensuring it meets the assumptions required for the chosen test.
• Calculate the test statistic and compare it to the critical value or p-value to determine significance.
• Interpret the results and draw conclusions about the research question.
• One-Sample Tests : These tests compare the mean or proportion of a single sample to a known or hypothesized value. Examples include the one-sample t-test and the one-sample z-test.
• Two-Sample Tests: These tests compare the means or proportions of two independent samples. Examples include the two-sample t-test, the Mann-Whitney U test, and the chi-square test of independence.
• Paired Tests: These tests compare the means or proportions of two related or paired samples, such as before-and-after measurements or matched pairs. Examples include the paired t-test and the Wilcoxon signed-rank test.
• ANOVA Tests : These tests compare the means of three or more independent samples. Examples include one-way ANOVA, two-way ANOVA, and repeated-measures ANOVA.
• Correlation and Regression Tests: These tests examine the relationship between two or more variables. Examples include Pearson’s correlation, Spearman’s rank correlation, and linear regression analysis.

While hypothesis testing is a powerful tool for data analysis, it is not immune to errors. Two common types of errors in hypothesis testing are Type I errors and Type II errors.

Type I Error

A Type I error occurs when the null hypothesis is true, but it is incorrectly rejected. The probability of making a Type I error is typically denoted by the significance level (α), which is the threshold used to determine statistical significance.

Type II Error

Conversely, a Type II error occurs when the null hypothesis is false, but it is not rejected. In this case, the researcher fails to detect a significant effect that is actually present. The probability of making a Type II error is denoted by β.

When a hypothesis test is conducted, the researcher is provided with a p-value, which represents the probability of obtaining the observed results if the null hypothesis is true. If the p-value is less than the chosen significance level (typically 0.05), the null hypothesis is rejected, and the alternative hypothesis is supported.

• Medicine and Pharmacology: Researchers use hypothesis testing to evaluate the effectiveness of new drugs, treatments, or interventions. For example, a clinical trial might test the null hypothesis that a new drug has no effect on a health outcome.
• Psychology and Behavioral Sciences: Psychologists use hypothesis testing to investigate human behavior, cognition, and social phenomena. For instance, a researcher might hypothesize that a new therapy has no effect on depression symptoms.
• Economics and Finance: Economists use hypothesis testing to evaluate market performance, economic policies, and investment strategies. For example, testing the hypothesis that interest rates have no effect on the stock market.
• Engineering and Technology: Engineers use hypothesis testing to optimize product designs, test system reliability, and evaluate new technologies. For example, testing a new manufacturing process to reduce defects.
• Environmental Science: Environmental scientists use hypothesis testing to evaluate the impact of human activities, conservation efforts, and climate change effects.

Various tools are available for conducting hypothesis tests:

• IBM SPSS Statistics : A user-friendly statistical software widely used for hypothesis testing.
• R : An open-source programming language for statistical computing, offering packages like stats , ggplot2 , and dplyr for hypothesis testing.
• Online Tools : Websites such as StatisticsHowTo.com offer hypothesis test calculators for quick analyses.

Hypothesis testing is a crucial tool for researchers across many disciplines. It allows them to make informed decisions, support or challenge theories, and contribute to knowledge advancement. By understanding and mastering hypothesis testing techniques, researchers can significantly enhance their data analysis impact.

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